Toxicological Impact and in Vivo Tracing of Rhodamine Functionalised ZIF-8 Nanoparticles
Metal Organic Frameworks (MOFs) are extensively used for a wide range of applications due to their exceptionally high surface area. MOF particles are conventionally in micron size, but the nanosized MOFs show good transportation/mobility due to their small size, and when combined with the high surfa...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Toxicology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/ftox.2022.917749/full |
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| author | Prateek Goyal Pushpanjali Soppina Pushpanjali Soppina Superb K. Misra Eugenia Valsami-Jones Virupakshi Soppina Swaroop Chakraborty |
| author_facet | Prateek Goyal Pushpanjali Soppina Pushpanjali Soppina Superb K. Misra Eugenia Valsami-Jones Virupakshi Soppina Swaroop Chakraborty |
| author_sort | Prateek Goyal |
| collection | DOAJ |
| description | Metal Organic Frameworks (MOFs) are extensively used for a wide range of applications due to their exceptionally high surface area. MOF particles are conventionally in micron size, but the nanosized MOFs show good transportation/mobility due to their small size, and when combined with the high surface area of MOFs, it makes MOF nanoparticles an ideal candidate to study for environmental remediation. Therefore, it is important to study the ecotoxicological impact of these MOFs. In this study, we developed rhodamine labelled nanoparticles of zinc imidazolate metal organic framework (ZIF-8 MOFs) as a means of in vivo tracing the MOF translocation in C. elegans. Rhodamine B isothiocyanate functionalized ZIF-8 MOFs nanoparticles (RBITC@ZIF-8 MOF nanoparticles; size 44 ± 7 nm) were fed to the worms naturally within a concentration range of 0.16–16.4 μg mg−1. Fluorescence was detected in the pharyngeal and gut lumen regions of the worms after 4 h of treatment, for exposure concentrations >0.163 μg mg−1. A higher intensity of fluorescence was observed at the end of 24 h for all exposure concentrations. Worms treated with RBITC@ZIF-8 MOF concentrations of ≥1.63 μg mg−1 for 24 h showed a bright stable fluorescence signal at the tail region. The uptake of RBITC@ZIF-8 MOF for an exposure concentration of 0.163, 1.63, and 8.2 μg mg−1 was found to be 52.1, 11.4 and 28.6%, respectively. Through this study, we showed that RBITC@ZIF-8 MOFs can be exposed to C. elegans and imaged at low concentrations of ∼0.16 μg mg−1. |
| first_indexed | 2024-12-12T07:12:43Z |
| format | Article |
| id | doaj.art-9d190f67054c42a4a82a112b56578cee |
| institution | Directory Open Access Journal |
| issn | 2673-3080 |
| language | English |
| last_indexed | 2024-12-12T07:12:43Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Toxicology |
| spelling | doaj.art-9d190f67054c42a4a82a112b56578cee2022-12-22T00:33:35ZengFrontiers Media S.A.Frontiers in Toxicology2673-30802022-07-01410.3389/ftox.2022.917749917749Toxicological Impact and in Vivo Tracing of Rhodamine Functionalised ZIF-8 NanoparticlesPrateek Goyal0Pushpanjali Soppina1Pushpanjali Soppina2Superb K. Misra3Eugenia Valsami-Jones4Virupakshi Soppina5Swaroop Chakraborty6Materials Engineering, Indian Institute of Technology Gandhinagar, Gandhinagar, IndiaBiotechnology and Bioinformatics, Sambalpur University, Burla, IndiaBiological Engineering, Indian Institute of Technology Gandhinagar, Gandhinagar, IndiaMaterials Engineering, Indian Institute of Technology Gandhinagar, Gandhinagar, IndiaSchool of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham, United KingdomBiological Engineering, Indian Institute of Technology Gandhinagar, Gandhinagar, IndiaSchool of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham, United KingdomMetal Organic Frameworks (MOFs) are extensively used for a wide range of applications due to their exceptionally high surface area. MOF particles are conventionally in micron size, but the nanosized MOFs show good transportation/mobility due to their small size, and when combined with the high surface area of MOFs, it makes MOF nanoparticles an ideal candidate to study for environmental remediation. Therefore, it is important to study the ecotoxicological impact of these MOFs. In this study, we developed rhodamine labelled nanoparticles of zinc imidazolate metal organic framework (ZIF-8 MOFs) as a means of in vivo tracing the MOF translocation in C. elegans. Rhodamine B isothiocyanate functionalized ZIF-8 MOFs nanoparticles (RBITC@ZIF-8 MOF nanoparticles; size 44 ± 7 nm) were fed to the worms naturally within a concentration range of 0.16–16.4 μg mg−1. Fluorescence was detected in the pharyngeal and gut lumen regions of the worms after 4 h of treatment, for exposure concentrations >0.163 μg mg−1. A higher intensity of fluorescence was observed at the end of 24 h for all exposure concentrations. Worms treated with RBITC@ZIF-8 MOF concentrations of ≥1.63 μg mg−1 for 24 h showed a bright stable fluorescence signal at the tail region. The uptake of RBITC@ZIF-8 MOF for an exposure concentration of 0.163, 1.63, and 8.2 μg mg−1 was found to be 52.1, 11.4 and 28.6%, respectively. Through this study, we showed that RBITC@ZIF-8 MOFs can be exposed to C. elegans and imaged at low concentrations of ∼0.16 μg mg−1.https://www.frontiersin.org/articles/10.3389/ftox.2022.917749/fullmetal organic frameworksfluorescent labelnanoparticles tracingnanotoxicity assessmentMOFs toxicityZIF-8 MOFs |
| spellingShingle | Prateek Goyal Pushpanjali Soppina Pushpanjali Soppina Superb K. Misra Eugenia Valsami-Jones Virupakshi Soppina Swaroop Chakraborty Toxicological Impact and in Vivo Tracing of Rhodamine Functionalised ZIF-8 Nanoparticles Frontiers in Toxicology metal organic frameworks fluorescent label nanoparticles tracing nanotoxicity assessment MOFs toxicity ZIF-8 MOFs |
| title | Toxicological Impact and in Vivo Tracing of Rhodamine Functionalised ZIF-8 Nanoparticles |
| title_full | Toxicological Impact and in Vivo Tracing of Rhodamine Functionalised ZIF-8 Nanoparticles |
| title_fullStr | Toxicological Impact and in Vivo Tracing of Rhodamine Functionalised ZIF-8 Nanoparticles |
| title_full_unstemmed | Toxicological Impact and in Vivo Tracing of Rhodamine Functionalised ZIF-8 Nanoparticles |
| title_short | Toxicological Impact and in Vivo Tracing of Rhodamine Functionalised ZIF-8 Nanoparticles |
| title_sort | toxicological impact and in vivo tracing of rhodamine functionalised zif 8 nanoparticles |
| topic | metal organic frameworks fluorescent label nanoparticles tracing nanotoxicity assessment MOFs toxicity ZIF-8 MOFs |
| url | https://www.frontiersin.org/articles/10.3389/ftox.2022.917749/full |
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