OPTIMIZATION OF BACTERIOCIN PRODUCTION BY Lactococcus lactis ssp. lactis CN1.10a ORIGIN FROM RUSIPS
Previous study of bacteriocin production on laboratory scale (100 mL) that used MRS broth medium produced unstable activity of bacteriocin. Therefore, this study aims to determine the optimum growth conditions and media for production of bacteriocin. Bacteria used in this research was a lactic acid...
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| Format: | Article |
| Language: | English |
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Kementerian Kelautan dan Perikanan
2014-12-01
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| Series: | Squalen |
| Subjects: | |
| Online Access: | http://bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/107 |
| _version_ | 1818363583272058880 |
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| author | Ninoek Indriati Arifah Kusmarwati Irma Hermana |
| author_facet | Ninoek Indriati Arifah Kusmarwati Irma Hermana |
| author_sort | Ninoek Indriati |
| collection | DOAJ |
| description | Previous study of bacteriocin production on laboratory scale (100 mL) that used MRS broth medium produced unstable activity of bacteriocin. Therefore, this study aims to determine the optimum growth conditions and media for production of bacteriocin. Bacteria used in this research was a lactic acid bacteria (LAB) Lactococcus lactis ssp. lactis CN1.10a isolated from rusip, a traditional Bangkanese fermented fish product.The bacteria was first cultivated for subsequent use of bacteriocins production on intermediate scale (2L). Followed by the optimization of temperature, pH and medium for the bacteriocin production, determination of cell growth curve, bacteriocin production curve, bacteriocin activity on that scale, and also stability of bacteriocin during storage.The results showed that the optimum temperature and pH for the growth of producer cell were 28°C and pH 6. The greatest activity of bacteriocin was produced on CM medium (1% sucrose, 0,45% peptone, 1% yeast extract, 2,84% KH2PO4, 0,2% NaCl and 0,02% MgSO4.7H20) in addition of sucrose as carbohydrate source. Based on the growth curve performedon CM medium with KH2PO4, the L. Lactis ssp lactis CN1.10a was relatively stable up to 48 hours. Bacteriocin produced by the cell was 8000 AU/mlat24th hour.Bacteriocin was relatively stable when stored at -20°C for 1month with a relative activity of 69,4%. |
| first_indexed | 2024-12-13T21:50:47Z |
| format | Article |
| id | doaj.art-9d1edababb3c4d61bd51422bd0bb0bb3 |
| institution | Directory Open Access Journal |
| issn | 2089-5690 2406-9272 |
| language | English |
| last_indexed | 2024-12-13T21:50:47Z |
| publishDate | 2014-12-01 |
| publisher | Kementerian Kelautan dan Perikanan |
| record_format | Article |
| series | Squalen |
| spelling | doaj.art-9d1edababb3c4d61bd51422bd0bb0bb32022-12-21T23:30:18ZengKementerian Kelautan dan PerikananSqualen2089-56902406-92722014-12-01939710610.15578/squalen.v9i3.10780OPTIMIZATION OF BACTERIOCIN PRODUCTION BY Lactococcus lactis ssp. lactis CN1.10a ORIGIN FROM RUSIPSNinoek Indriati0Arifah Kusmarwati1Irma Hermana2Research and Development Center for Marine and Fisheries Product Processing and BiotechnologyResearch and Development Center for Marine and Fisheries Product Processing and BiotechnologyResearch and Development Center for Marine and Fisheries Product Processing and BiotechnologyPrevious study of bacteriocin production on laboratory scale (100 mL) that used MRS broth medium produced unstable activity of bacteriocin. Therefore, this study aims to determine the optimum growth conditions and media for production of bacteriocin. Bacteria used in this research was a lactic acid bacteria (LAB) Lactococcus lactis ssp. lactis CN1.10a isolated from rusip, a traditional Bangkanese fermented fish product.The bacteria was first cultivated for subsequent use of bacteriocins production on intermediate scale (2L). Followed by the optimization of temperature, pH and medium for the bacteriocin production, determination of cell growth curve, bacteriocin production curve, bacteriocin activity on that scale, and also stability of bacteriocin during storage.The results showed that the optimum temperature and pH for the growth of producer cell were 28°C and pH 6. The greatest activity of bacteriocin was produced on CM medium (1% sucrose, 0,45% peptone, 1% yeast extract, 2,84% KH2PO4, 0,2% NaCl and 0,02% MgSO4.7H20) in addition of sucrose as carbohydrate source. Based on the growth curve performedon CM medium with KH2PO4, the L. Lactis ssp lactis CN1.10a was relatively stable up to 48 hours. Bacteriocin produced by the cell was 8000 AU/mlat24th hour.Bacteriocin was relatively stable when stored at -20°C for 1month with a relative activity of 69,4%.http://bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/107optimization, cell growth curve, production of bacteriocins, Lactococcus lactis, rusip |
| spellingShingle | Ninoek Indriati Arifah Kusmarwati Irma Hermana OPTIMIZATION OF BACTERIOCIN PRODUCTION BY Lactococcus lactis ssp. lactis CN1.10a ORIGIN FROM RUSIPS Squalen optimization, cell growth curve, production of bacteriocins, Lactococcus lactis, rusip |
| title | OPTIMIZATION OF BACTERIOCIN PRODUCTION BY Lactococcus lactis ssp. lactis CN1.10a ORIGIN FROM RUSIPS |
| title_full | OPTIMIZATION OF BACTERIOCIN PRODUCTION BY Lactococcus lactis ssp. lactis CN1.10a ORIGIN FROM RUSIPS |
| title_fullStr | OPTIMIZATION OF BACTERIOCIN PRODUCTION BY Lactococcus lactis ssp. lactis CN1.10a ORIGIN FROM RUSIPS |
| title_full_unstemmed | OPTIMIZATION OF BACTERIOCIN PRODUCTION BY Lactococcus lactis ssp. lactis CN1.10a ORIGIN FROM RUSIPS |
| title_short | OPTIMIZATION OF BACTERIOCIN PRODUCTION BY Lactococcus lactis ssp. lactis CN1.10a ORIGIN FROM RUSIPS |
| title_sort | optimization of bacteriocin production by lactococcus lactis ssp lactis cn1 10a origin from rusips |
| topic | optimization, cell growth curve, production of bacteriocins, Lactococcus lactis, rusip |
| url | http://bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/107 |
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