The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and Metastasis
Colorectal cancer (CRC) is one of the most common and deadly cancers in the world. However, no effective treatment for the disease has yet been found. For this reason, several studies are being carried out on the treatment of CRC. Currently, there is limited understanding of the role of <i>CPN...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/23/16704 |
| Summary: | Colorectal cancer (CRC) is one of the most common and deadly cancers in the world. However, no effective treatment for the disease has yet been found. For this reason, several studies are being carried out on the treatment of CRC. Currently, there is limited understanding of the role of <i>CPNE7</i> (copine-7) in CRC progression and metastasis. The results of this study show that <i>CPNE7</i> exerts an oncogenic effect in CRC. First, <i>CPNE7</i> was shown to be significantly up-regulated in CRC patient tissues and CRC cell lines compared to normal tissues according to IHC staining, qRT-PCR, and western blotting. Next, this study used both systems of siRNA and shRNA to suppress <i>CPNE</i>7 gene expression to check the <i>CPNE7</i> mechanism in CRC. The suppressed <i>CPNE7</i> significantly inhibited the growth of CRC cells in in vitro experiments, including migration, invasion, and semisolid agar colony-forming assay. Moreover, the modified expression of <i>CPNE7</i> led to a decrease in the levels of genes associated with epithelial–mesenchymal transition (EMT). The epithelial genes <i>E-cadherin</i> (<i>CDH1</i>) and <i>Collagen A1</i> were upregulated, and the levels of mesenchymal genes such as <i>N-cadherin (CDH2)</i>, <i>ZEB1</i>, <i>ZEB2</i>, and <i>SNAIL</i> (<i>SNAL1</i>) were downregulated after <i>CPNE7</i> inhibition. This study suggests that <i>CPNE7</i> may serve as a potential diagnostic biomarker for CRC patients. |
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| ISSN: | 1661-6596 1422-0067 |