The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and Metastasis
Colorectal cancer (CRC) is one of the most common and deadly cancers in the world. However, no effective treatment for the disease has yet been found. For this reason, several studies are being carried out on the treatment of CRC. Currently, there is limited understanding of the role of <i>CPN...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/23/16704 |
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| author | Hye-Jeong Kong Dong-Hyun Kang Tae-Sung Ahn Kwang-Seock Kim Tae-Wan Kim Soo-Hyeon Lee Dong-Woo Lee Jae-Sung Ryu Moo-Jun Beak |
| author_facet | Hye-Jeong Kong Dong-Hyun Kang Tae-Sung Ahn Kwang-Seock Kim Tae-Wan Kim Soo-Hyeon Lee Dong-Woo Lee Jae-Sung Ryu Moo-Jun Beak |
| author_sort | Hye-Jeong Kong |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is one of the most common and deadly cancers in the world. However, no effective treatment for the disease has yet been found. For this reason, several studies are being carried out on the treatment of CRC. Currently, there is limited understanding of the role of <i>CPNE7</i> (copine-7) in CRC progression and metastasis. The results of this study show that <i>CPNE7</i> exerts an oncogenic effect in CRC. First, <i>CPNE7</i> was shown to be significantly up-regulated in CRC patient tissues and CRC cell lines compared to normal tissues according to IHC staining, qRT-PCR, and western blotting. Next, this study used both systems of siRNA and shRNA to suppress <i>CPNE</i>7 gene expression to check the <i>CPNE7</i> mechanism in CRC. The suppressed <i>CPNE7</i> significantly inhibited the growth of CRC cells in in vitro experiments, including migration, invasion, and semisolid agar colony-forming assay. Moreover, the modified expression of <i>CPNE7</i> led to a decrease in the levels of genes associated with epithelial–mesenchymal transition (EMT). The epithelial genes <i>E-cadherin</i> (<i>CDH1</i>) and <i>Collagen A1</i> were upregulated, and the levels of mesenchymal genes such as <i>N-cadherin (CDH2)</i>, <i>ZEB1</i>, <i>ZEB2</i>, and <i>SNAIL</i> (<i>SNAL1</i>) were downregulated after <i>CPNE7</i> inhibition. This study suggests that <i>CPNE7</i> may serve as a potential diagnostic biomarker for CRC patients. |
| first_indexed | 2024-03-09T01:50:05Z |
| format | Article |
| id | doaj.art-9d23cb0aad384c34959fa639d52ed781 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:50:05Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9d23cb0aad384c34959fa639d52ed7812023-12-08T15:16:50ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124231670410.3390/ijms242316704The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and MetastasisHye-Jeong Kong0Dong-Hyun Kang1Tae-Sung Ahn2Kwang-Seock Kim3Tae-Wan Kim4Soo-Hyeon Lee5Dong-Woo Lee6Jae-Sung Ryu7Moo-Jun Beak8Department of Medical Life Science, Soonchunhyang University, Asan 31538, Republic of KoreaDepartment of Surgery, Soonchunhyang University College of Medicine, Soonchunhyang University Cheonan Hospital, Cheonan 31151, Republic of KoreaDepartment of Surgery, Soonchunhyang University College of Medicine, Soonchunhyang University Cheonan Hospital, Cheonan 31151, Republic of KoreaDepartment of Medical Life Science, Soonchunhyang University, Asan 31538, Republic of KoreaDepartment of Medical Life Science, Soonchunhyang University, Asan 31538, Republic of KoreaDepartment of Surgery, Soonchunhyang University College of Medicine, Soonchunhyang University Cheonan Hospital, Cheonan 31151, Republic of KoreaDepartment of Medical Life Science, Soonchunhyang University, Asan 31538, Republic of KoreaDepartment of Medical Life Science, Soonchunhyang University, Asan 31538, Republic of KoreaDepartment of Surgery, Soonchunhyang University College of Medicine, Soonchunhyang University Cheonan Hospital, Cheonan 31151, Republic of KoreaColorectal cancer (CRC) is one of the most common and deadly cancers in the world. However, no effective treatment for the disease has yet been found. For this reason, several studies are being carried out on the treatment of CRC. Currently, there is limited understanding of the role of <i>CPNE7</i> (copine-7) in CRC progression and metastasis. The results of this study show that <i>CPNE7</i> exerts an oncogenic effect in CRC. First, <i>CPNE7</i> was shown to be significantly up-regulated in CRC patient tissues and CRC cell lines compared to normal tissues according to IHC staining, qRT-PCR, and western blotting. Next, this study used both systems of siRNA and shRNA to suppress <i>CPNE</i>7 gene expression to check the <i>CPNE7</i> mechanism in CRC. The suppressed <i>CPNE7</i> significantly inhibited the growth of CRC cells in in vitro experiments, including migration, invasion, and semisolid agar colony-forming assay. Moreover, the modified expression of <i>CPNE7</i> led to a decrease in the levels of genes associated with epithelial–mesenchymal transition (EMT). The epithelial genes <i>E-cadherin</i> (<i>CDH1</i>) and <i>Collagen A1</i> were upregulated, and the levels of mesenchymal genes such as <i>N-cadherin (CDH2)</i>, <i>ZEB1</i>, <i>ZEB2</i>, and <i>SNAIL</i> (<i>SNAL1</i>) were downregulated after <i>CPNE7</i> inhibition. This study suggests that <i>CPNE7</i> may serve as a potential diagnostic biomarker for CRC patients.https://www.mdpi.com/1422-0067/24/23/16704colorectal cancer<i>CPNE7</i>siRNAshRNAEMT<i>E-cadherin</i> |
| spellingShingle | Hye-Jeong Kong Dong-Hyun Kang Tae-Sung Ahn Kwang-Seock Kim Tae-Wan Kim Soo-Hyeon Lee Dong-Woo Lee Jae-Sung Ryu Moo-Jun Beak The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and Metastasis International Journal of Molecular Sciences colorectal cancer <i>CPNE7</i> siRNA shRNA EMT <i>E-cadherin</i> |
| title | The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and Metastasis |
| title_full | The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and Metastasis |
| title_fullStr | The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and Metastasis |
| title_full_unstemmed | The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and Metastasis |
| title_short | The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and Metastasis |
| title_sort | role of cpne7 copine 7 in colorectal cancer prognosis and metastasis |
| topic | colorectal cancer <i>CPNE7</i> siRNA shRNA EMT <i>E-cadherin</i> |
| url | https://www.mdpi.com/1422-0067/24/23/16704 |
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