DetectionOf genesResponsible for BiofilmsFormedby Klebsiella pneumoniaeandEsherichia coliand their effect on innate immunity
Biofilms as a major virulence factor of bacteria ,therelationship between bacteria persistence in the urinary tract and genes responsible for biofilms production was studied. The aim is to detect the presence,frequency of the,fimA, fimH, mrkA and mrkD, genes and biofilms production in two speci...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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College of Pharmacy / Mustansiriyah University
2018-03-01
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| Series: | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/75 |
| _version_ | 1797223939554934784 |
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| author | Ali Hussein Alwan Noor Naeem Khwen |
| author_facet | Ali Hussein Alwan Noor Naeem Khwen |
| author_sort | Ali Hussein Alwan |
| collection | DOAJ |
| description |
Biofilms as a major virulence factor of bacteria ,therelationship between bacteria persistence in the urinary tract and genes responsible for biofilms production was studied. The aim is to detect the presence,frequency of the,fimA, fimH, mrkA and mrkD, genes and biofilms production in two species isolated from UTI patients and their effect on some innate immunity aspects. Sixty five isolates of E.coli and fifty of K.pneumoniae isolates were collected. All isolates were initially diagnosed as genus Esherichiacoli andKlebsiella pneumonia. Final identification for the isolates was done by Vitek 2 compact system. The ability to form biofilm was carried out by using Tissue culture plate method (TCP).Adhesion average to epithelial cells of E.coli and K.pneumoniae was studied. Detection of genes were done to by using PCR technique . Immunological experiments were done by using bactericidal activity and opsonization factor. UTI infection were more common in female than male. 75% (49/65) females, 25%(16/65) males ofE.coli isolates and 88% (44/50) females,12%(6/50) males of K.pneumoniaeisolates. Patients ages in children between (2 months -18years) in UTI caused by E.coli was 35%(23/65) while in k.pneumoniae was 74%(37/50),Ages between (18 years-50 years) in E.coli was 37%(24/65) while in K.pneumoniae was 18%(9/50) finally,Ages between (50 years-72years) in E.coli was 28%(18/65) while in K.pneumoniae was 8%(4/50).69% of E.coli isolates and 72% of k.pneumoniae isolates were higher biofilm producers. . Adhesion average to epithial cells of E.coli and K.pneumoniae was 42.9% and 44% respectively. Both E.coli and K.pneumoniae showed that 100% 0f the 12 isolate harbored the fimA, fimH, mrkA and mrkD genes. The bactericidal average was higher in E.coli than K.pneumoniae , P- value( 0.01) for mice injected with fimbriae, Opsonization factor was higher in E.coli than K.pneumoniae the P value (0.05) . Conclusion: A strong relationship between biofilms production and presence of genes of different species isolated from same clinical source that has effected on resistance of bacteria to innate immunity.
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| first_indexed | 2024-04-24T13:45:11Z |
| format | Article |
| id | doaj.art-9d27116140d34dc2956f09775cb88bd7 |
| institution | Directory Open Access Journal |
| issn | 1815-0993 2959-183X |
| language | English |
| last_indexed | 2024-04-24T13:45:11Z |
| publishDate | 2018-03-01 |
| publisher | College of Pharmacy / Mustansiriyah University |
| record_format | Article |
| series | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
| spelling | doaj.art-9d27116140d34dc2956f09775cb88bd72024-04-04T06:58:07ZengCollege of Pharmacy / Mustansiriyah UniversityAl-Mustansiriyah Journal of Pharmaceutical Sciences1815-09932959-183X2018-03-0117110.32947/ajps.v17i1.75DetectionOf genesResponsible for BiofilmsFormedby Klebsiella pneumoniaeandEsherichia coliand their effect on innate immunityAli Hussein Alwan0Noor Naeem Khwen1Department of Biology/ Collage of science, Al-Mustansiryah University/ Baghdad /IraqDepartment of Biology/ Collage of science, Al-Mustansiryah University/ Baghdad /Iraq Biofilms as a major virulence factor of bacteria ,therelationship between bacteria persistence in the urinary tract and genes responsible for biofilms production was studied. The aim is to detect the presence,frequency of the,fimA, fimH, mrkA and mrkD, genes and biofilms production in two species isolated from UTI patients and their effect on some innate immunity aspects. Sixty five isolates of E.coli and fifty of K.pneumoniae isolates were collected. All isolates were initially diagnosed as genus Esherichiacoli andKlebsiella pneumonia. Final identification for the isolates was done by Vitek 2 compact system. The ability to form biofilm was carried out by using Tissue culture plate method (TCP).Adhesion average to epithelial cells of E.coli and K.pneumoniae was studied. Detection of genes were done to by using PCR technique . Immunological experiments were done by using bactericidal activity and opsonization factor. UTI infection were more common in female than male. 75% (49/65) females, 25%(16/65) males ofE.coli isolates and 88% (44/50) females,12%(6/50) males of K.pneumoniaeisolates. Patients ages in children between (2 months -18years) in UTI caused by E.coli was 35%(23/65) while in k.pneumoniae was 74%(37/50),Ages between (18 years-50 years) in E.coli was 37%(24/65) while in K.pneumoniae was 18%(9/50) finally,Ages between (50 years-72years) in E.coli was 28%(18/65) while in K.pneumoniae was 8%(4/50).69% of E.coli isolates and 72% of k.pneumoniae isolates were higher biofilm producers. . Adhesion average to epithial cells of E.coli and K.pneumoniae was 42.9% and 44% respectively. Both E.coli and K.pneumoniae showed that 100% 0f the 12 isolate harbored the fimA, fimH, mrkA and mrkD genes. The bactericidal average was higher in E.coli than K.pneumoniae , P- value( 0.01) for mice injected with fimbriae, Opsonization factor was higher in E.coli than K.pneumoniae the P value (0.05) . Conclusion: A strong relationship between biofilms production and presence of genes of different species isolated from same clinical source that has effected on resistance of bacteria to innate immunity. https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/75K.pneumoniaeE.colifimAfimHmrkAmrkD |
| spellingShingle | Ali Hussein Alwan Noor Naeem Khwen DetectionOf genesResponsible for BiofilmsFormedby Klebsiella pneumoniaeandEsherichia coliand their effect on innate immunity Al-Mustansiriyah Journal of Pharmaceutical Sciences K.pneumoniae E.coli fimA fimH mrkA mrkD |
| title | DetectionOf genesResponsible for BiofilmsFormedby Klebsiella pneumoniaeandEsherichia coliand their effect on innate immunity |
| title_full | DetectionOf genesResponsible for BiofilmsFormedby Klebsiella pneumoniaeandEsherichia coliand their effect on innate immunity |
| title_fullStr | DetectionOf genesResponsible for BiofilmsFormedby Klebsiella pneumoniaeandEsherichia coliand their effect on innate immunity |
| title_full_unstemmed | DetectionOf genesResponsible for BiofilmsFormedby Klebsiella pneumoniaeandEsherichia coliand their effect on innate immunity |
| title_short | DetectionOf genesResponsible for BiofilmsFormedby Klebsiella pneumoniaeandEsherichia coliand their effect on innate immunity |
| title_sort | detectionof genesresponsible for biofilmsformedby klebsiella pneumoniaeandesherichia coliand their effect on innate immunity |
| topic | K.pneumoniae E.coli fimA fimH mrkA mrkD |
| url | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/75 |
| work_keys_str_mv | AT alihusseinalwan detectionofgenesresponsibleforbiofilmsformedbyklebsiellapneumoniaeandesherichiacoliandtheireffectoninnateimmunity AT noornaeemkhwen detectionofgenesresponsibleforbiofilmsformedbyklebsiellapneumoniaeandesherichiacoliandtheireffectoninnateimmunity |