The cuticle inward barrier in Drosophila melanogaster is shaped by mitochondrial and nuclear genotypes and a sex-specific effect of diet
An important role of the insect cuticle is to prevent wetting (i.e., permeation of water) and also to prevent penetration of potentially harmful substances. This barrier function mainly depends on the hydrophobic cuticle surface composed of lipids including cuticular hydrocarbons (CHCs). We investig...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2019-10-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/7802.pdf |
| _version_ | 1797420571359707136 |
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| author | Wei Dong Ralph Dobler Damian K. Dowling Bernard Moussian |
| author_facet | Wei Dong Ralph Dobler Damian K. Dowling Bernard Moussian |
| author_sort | Wei Dong |
| collection | DOAJ |
| description | An important role of the insect cuticle is to prevent wetting (i.e., permeation of water) and also to prevent penetration of potentially harmful substances. This barrier function mainly depends on the hydrophobic cuticle surface composed of lipids including cuticular hydrocarbons (CHCs). We investigated to what extent the cuticle inward barrier function depends on the genotype, comprising mitochondrial and nuclear genes in the fruit fly Drosophila melanogaster, and investigated the contribution of interactions between mitochondrial and nuclear genotypes (mito-nuclear interactions) on this function. In addition, we assessed the effects of nutrition and sex on the cuticle barrier function. Based on a dye penetration assay, we find that cuticle barrier function varies across three fly lines that were captured from geographically separated regions in three continents. Testing different combinations of mito-nuclear genotypes, we show that the inward barrier efficiency is modulated by the nuclear and mitochondrial genomes independently. We also find an interaction between diet and sex. Our findings provide new insights into the regulation of cuticle inward barrier function in nature. |
| first_indexed | 2024-03-09T07:04:22Z |
| format | Article |
| id | doaj.art-9d284014e88a4e59b4842f7db8241fd9 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:04:22Z |
| publishDate | 2019-10-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
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| spelling | doaj.art-9d284014e88a4e59b4842f7db8241fd92023-12-03T09:45:54ZengPeerJ Inc.PeerJ2167-83592019-10-017e780210.7717/peerj.7802The cuticle inward barrier in Drosophila melanogaster is shaped by mitochondrial and nuclear genotypes and a sex-specific effect of dietWei Dong0Ralph Dobler1Damian K. Dowling2Bernard Moussian3Institute of Applied Biology, Shanxi University, Taiyuan, ChinaApplied Zoology, Faculty of Biology, Technische Universität Dresden, Dresden, GermanySchool of Biological Sciences, Monash University, Clayton, Victoria, AustraliaInstitute of Applied Biology, Shanxi University, Taiyuan, ChinaAn important role of the insect cuticle is to prevent wetting (i.e., permeation of water) and also to prevent penetration of potentially harmful substances. This barrier function mainly depends on the hydrophobic cuticle surface composed of lipids including cuticular hydrocarbons (CHCs). We investigated to what extent the cuticle inward barrier function depends on the genotype, comprising mitochondrial and nuclear genes in the fruit fly Drosophila melanogaster, and investigated the contribution of interactions between mitochondrial and nuclear genotypes (mito-nuclear interactions) on this function. In addition, we assessed the effects of nutrition and sex on the cuticle barrier function. Based on a dye penetration assay, we find that cuticle barrier function varies across three fly lines that were captured from geographically separated regions in three continents. Testing different combinations of mito-nuclear genotypes, we show that the inward barrier efficiency is modulated by the nuclear and mitochondrial genomes independently. We also find an interaction between diet and sex. Our findings provide new insights into the regulation of cuticle inward barrier function in nature.https://peerj.com/articles/7802.pdfMitonuclearMito-nuclearMitochondriaDesiccationXenobioticsmtDNA |
| spellingShingle | Wei Dong Ralph Dobler Damian K. Dowling Bernard Moussian The cuticle inward barrier in Drosophila melanogaster is shaped by mitochondrial and nuclear genotypes and a sex-specific effect of diet PeerJ Mitonuclear Mito-nuclear Mitochondria Desiccation Xenobiotics mtDNA |
| title | The cuticle inward barrier in Drosophila melanogaster is shaped by mitochondrial and nuclear genotypes and a sex-specific effect of diet |
| title_full | The cuticle inward barrier in Drosophila melanogaster is shaped by mitochondrial and nuclear genotypes and a sex-specific effect of diet |
| title_fullStr | The cuticle inward barrier in Drosophila melanogaster is shaped by mitochondrial and nuclear genotypes and a sex-specific effect of diet |
| title_full_unstemmed | The cuticle inward barrier in Drosophila melanogaster is shaped by mitochondrial and nuclear genotypes and a sex-specific effect of diet |
| title_short | The cuticle inward barrier in Drosophila melanogaster is shaped by mitochondrial and nuclear genotypes and a sex-specific effect of diet |
| title_sort | cuticle inward barrier in drosophila melanogaster is shaped by mitochondrial and nuclear genotypes and a sex specific effect of diet |
| topic | Mitonuclear Mito-nuclear Mitochondria Desiccation Xenobiotics mtDNA |
| url | https://peerj.com/articles/7802.pdf |
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