Hydrogen sulfide protects retina from blue light-induced photodamage and degeneration via inhibiting ROS-mediated ER stress-CHOP apoptosis signal
Background: Hydrogen sulfide (H2S) is a small reducing gas molecule with various biological functions such as anti-oxidative, anti-apoptotic and anti-inflammatory activities. In this study, we investigated the therapeutic effects of exogenous H2S in the experimental models of retinal photodamage in...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Redox Report |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13510002.2022.2069534 |
| _version_ | 1818009468208676864 |
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| author | Sen Zhu Xuan Li Bingrong Dang Fen Wu Kexin Gou Chunming Wang Changjun Lin |
| author_facet | Sen Zhu Xuan Li Bingrong Dang Fen Wu Kexin Gou Chunming Wang Changjun Lin |
| author_sort | Sen Zhu |
| collection | DOAJ |
| description | Background: Hydrogen sulfide (H2S) is a small reducing gas molecule with various biological functions such as anti-oxidative, anti-apoptotic and anti-inflammatory activities. In this study, we investigated the therapeutic effects of exogenous H2S in the experimental models of retinal photodamage in vivo and in vitro.Methods: Rats with open eyelids were pretreated with H2S (80~120 μmol/kg) for 10 days and then continuously exposed to blue light (435~445nm, 11.2W/m2) for 8 h to establish in vivo experimental model. ARPE-19 cells were pretreated with H2S and then exposed to blue light to establish in vitro experimental model.Results: In vivo experiments, H2S significantly ameliorated blue light-induced retinal oxidative stress, apoptosis and degeneration. Moreover, H2S inhibited the activation of blue light-induced endoplasmic reticulum (ER) stress CHOP apoptotic signaling. In vitro experiments, H2S improved blue light-induced oxidative stress and oxidative damage. H2S inhibited ROS-mediated activation of ER stress CHOP apoptotic signaling. H2S alleviated blue light-induced apoptosis and increases cell viability. The ER stress inhibitor 4-PBA alleviated blue light-induced apoptosis and increases cell viability.Conclusion: Taken together, these results indicate that H2S can inhibit ROS-mediated ER stress-CHOP apoptosis signal, thereby alleviating blue light-triggered retinal apoptosis and degeneration. |
| first_indexed | 2024-04-14T05:42:19Z |
| format | Article |
| id | doaj.art-9d29797b369a4c57a4efbe849f35d61a |
| institution | Directory Open Access Journal |
| issn | 1351-0002 1743-2928 |
| language | English |
| last_indexed | 2024-04-14T05:42:19Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Redox Report |
| spelling | doaj.art-9d29797b369a4c57a4efbe849f35d61a2022-12-22T02:09:24ZengTaylor & Francis GroupRedox Report1351-00021743-29282022-12-0127110011010.1080/13510002.2022.2069534Hydrogen sulfide protects retina from blue light-induced photodamage and degeneration via inhibiting ROS-mediated ER stress-CHOP apoptosis signalSen Zhu0Xuan Li1Bingrong Dang2Fen Wu3Kexin Gou4Chunming Wang5Changjun Lin6School of Life Sciences, Lanzhou University, Lanzhou, People’s Republic of ChinaLanzhou University Second Hospital, Lanzhou, People’s Republic of ChinaChinese Academy of Sciences, Institute of Modern Physics, Lanzhou, People’s Republic of ChinaSchool of Life Sciences, Lanzhou University, Lanzhou, People’s Republic of ChinaSchool of Life Sciences, Lanzhou University, Lanzhou, People’s Republic of ChinaSchool of Life Sciences, Lanzhou University, Lanzhou, People’s Republic of ChinaSchool of Life Sciences, Lanzhou University, Lanzhou, People’s Republic of ChinaBackground: Hydrogen sulfide (H2S) is a small reducing gas molecule with various biological functions such as anti-oxidative, anti-apoptotic and anti-inflammatory activities. In this study, we investigated the therapeutic effects of exogenous H2S in the experimental models of retinal photodamage in vivo and in vitro.Methods: Rats with open eyelids were pretreated with H2S (80~120 μmol/kg) for 10 days and then continuously exposed to blue light (435~445nm, 11.2W/m2) for 8 h to establish in vivo experimental model. ARPE-19 cells were pretreated with H2S and then exposed to blue light to establish in vitro experimental model.Results: In vivo experiments, H2S significantly ameliorated blue light-induced retinal oxidative stress, apoptosis and degeneration. Moreover, H2S inhibited the activation of blue light-induced endoplasmic reticulum (ER) stress CHOP apoptotic signaling. In vitro experiments, H2S improved blue light-induced oxidative stress and oxidative damage. H2S inhibited ROS-mediated activation of ER stress CHOP apoptotic signaling. H2S alleviated blue light-induced apoptosis and increases cell viability. The ER stress inhibitor 4-PBA alleviated blue light-induced apoptosis and increases cell viability.Conclusion: Taken together, these results indicate that H2S can inhibit ROS-mediated ER stress-CHOP apoptosis signal, thereby alleviating blue light-triggered retinal apoptosis and degeneration.https://www.tandfonline.com/doi/10.1080/13510002.2022.2069534H2Sphotodamageretinal degenerationoxidative damageendoplasmic reticulum stressreactive oxygen species |
| spellingShingle | Sen Zhu Xuan Li Bingrong Dang Fen Wu Kexin Gou Chunming Wang Changjun Lin Hydrogen sulfide protects retina from blue light-induced photodamage and degeneration via inhibiting ROS-mediated ER stress-CHOP apoptosis signal Redox Report H2S photodamage retinal degeneration oxidative damage endoplasmic reticulum stress reactive oxygen species |
| title | Hydrogen sulfide protects retina from blue light-induced photodamage and degeneration via inhibiting ROS-mediated ER stress-CHOP apoptosis signal |
| title_full | Hydrogen sulfide protects retina from blue light-induced photodamage and degeneration via inhibiting ROS-mediated ER stress-CHOP apoptosis signal |
| title_fullStr | Hydrogen sulfide protects retina from blue light-induced photodamage and degeneration via inhibiting ROS-mediated ER stress-CHOP apoptosis signal |
| title_full_unstemmed | Hydrogen sulfide protects retina from blue light-induced photodamage and degeneration via inhibiting ROS-mediated ER stress-CHOP apoptosis signal |
| title_short | Hydrogen sulfide protects retina from blue light-induced photodamage and degeneration via inhibiting ROS-mediated ER stress-CHOP apoptosis signal |
| title_sort | hydrogen sulfide protects retina from blue light induced photodamage and degeneration via inhibiting ros mediated er stress chop apoptosis signal |
| topic | H2S photodamage retinal degeneration oxidative damage endoplasmic reticulum stress reactive oxygen species |
| url | https://www.tandfonline.com/doi/10.1080/13510002.2022.2069534 |
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