Novel tumor-targeting, self-assembling peptide nanofiber as a carrier for effective curcumin delivery
Jianfeng Liu, Jinjian Liu, Hongyan Xu, Yumin Zhang, Liping Chu, Qingfen Liu, Naling Song, Cuihong YangTianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, People's Republic of ChinaAbstract...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2013-12-01
|
| Series: | International Journal of Nanomedicine |
| Online Access: | http://www.dovepress.com/novel-tumor-targeting-self-assembling-peptide-nanofiber-as-a-carrier-f-a15367 |
| _version_ | 1818905360141910016 |
|---|---|
| author | Liu J Liu J Xu H Zhang Y Chu L Liu Q Song N Yang C |
| author_facet | Liu J Liu J Xu H Zhang Y Chu L Liu Q Song N Yang C |
| author_sort | Liu J |
| collection | DOAJ |
| description | Jianfeng Liu, Jinjian Liu, Hongyan Xu, Yumin Zhang, Liping Chu, Qingfen Liu, Naling Song, Cuihong YangTianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, People's Republic of ChinaAbstract: The poor aqueous solubility and low bioavailability of curcumin restrict its clinical application for cancer treatment. In this study, a novel tumor-targeting nanofiber carrier was developed to improve the solubility and tumor-targeting ability of curcumin using a self-assembled Nap-GFFYG-RGD peptide. The morphologies of the peptide nanofiber and the curcumin-encapsulated nanofiber were visualized by transmission electron microscopy. The tumor-targeting activity of the curcumin-encapsulated Nap-GFFYG-RGD peptide nanofiber (f-RGD-Cur) was studied in vitro and in vivo, using Nap-GFFYG-RGE peptide nanofiber (f-RGE-Cur) as the control. Curcumin was encapsulated into the peptide nanofiber, which had a diameter of approximately 10–20 nm. Curcumin showed sustained-release behavior from the nanofibers in vitro. f-RGD-Cur showed much higher cellular uptake in αvβ3 integrin-positive HepG2 liver carcinoma cells than did non-targeted f-RGE-Cur, thereby leading to significantly higher cytotoxicity. Ex vivo studies further demonstrated that curcumin could accumulate markedly in mouse tumors after administration of f-RGD-Cur via the tail vein. These results indicate that Nap-GFFYG-RGD peptide self-assembled nanofibers are a promising hydrophobic drug delivery system for targeted treatment of cancer.Keywords: nanofiber, tumor-targeting, self-assembling, curcumin, drug delivery |
| first_indexed | 2024-12-19T21:22:05Z |
| format | Article |
| id | doaj.art-9d2b48646b6c4837a77c18f44a26c8fa |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-19T21:22:05Z |
| publishDate | 2013-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-9d2b48646b6c4837a77c18f44a26c8fa2022-12-21T20:05:13ZengDove Medical PressInternational Journal of Nanomedicine1178-20132013-12-012014Issue 119720715367Novel tumor-targeting, self-assembling peptide nanofiber as a carrier for effective curcumin deliveryLiu JLiu JXu HZhang YChu LLiu QSong NYang CJianfeng Liu, Jinjian Liu, Hongyan Xu, Yumin Zhang, Liping Chu, Qingfen Liu, Naling Song, Cuihong YangTianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, People's Republic of ChinaAbstract: The poor aqueous solubility and low bioavailability of curcumin restrict its clinical application for cancer treatment. In this study, a novel tumor-targeting nanofiber carrier was developed to improve the solubility and tumor-targeting ability of curcumin using a self-assembled Nap-GFFYG-RGD peptide. The morphologies of the peptide nanofiber and the curcumin-encapsulated nanofiber were visualized by transmission electron microscopy. The tumor-targeting activity of the curcumin-encapsulated Nap-GFFYG-RGD peptide nanofiber (f-RGD-Cur) was studied in vitro and in vivo, using Nap-GFFYG-RGE peptide nanofiber (f-RGE-Cur) as the control. Curcumin was encapsulated into the peptide nanofiber, which had a diameter of approximately 10–20 nm. Curcumin showed sustained-release behavior from the nanofibers in vitro. f-RGD-Cur showed much higher cellular uptake in αvβ3 integrin-positive HepG2 liver carcinoma cells than did non-targeted f-RGE-Cur, thereby leading to significantly higher cytotoxicity. Ex vivo studies further demonstrated that curcumin could accumulate markedly in mouse tumors after administration of f-RGD-Cur via the tail vein. These results indicate that Nap-GFFYG-RGD peptide self-assembled nanofibers are a promising hydrophobic drug delivery system for targeted treatment of cancer.Keywords: nanofiber, tumor-targeting, self-assembling, curcumin, drug deliveryhttp://www.dovepress.com/novel-tumor-targeting-self-assembling-peptide-nanofiber-as-a-carrier-f-a15367 |
| spellingShingle | Liu J Liu J Xu H Zhang Y Chu L Liu Q Song N Yang C Novel tumor-targeting, self-assembling peptide nanofiber as a carrier for effective curcumin delivery International Journal of Nanomedicine |
| title | Novel tumor-targeting, self-assembling peptide nanofiber as a carrier for effective curcumin delivery |
| title_full | Novel tumor-targeting, self-assembling peptide nanofiber as a carrier for effective curcumin delivery |
| title_fullStr | Novel tumor-targeting, self-assembling peptide nanofiber as a carrier for effective curcumin delivery |
| title_full_unstemmed | Novel tumor-targeting, self-assembling peptide nanofiber as a carrier for effective curcumin delivery |
| title_short | Novel tumor-targeting, self-assembling peptide nanofiber as a carrier for effective curcumin delivery |
| title_sort | novel tumor targeting self assembling peptide nanofiber as a carrier for effective curcumin delivery |
| url | http://www.dovepress.com/novel-tumor-targeting-self-assembling-peptide-nanofiber-as-a-carrier-f-a15367 |
| work_keys_str_mv | AT liuj noveltumortargetingselfassemblingpeptidenanofiberasacarrierforeffectivecurcumindelivery AT liuj noveltumortargetingselfassemblingpeptidenanofiberasacarrierforeffectivecurcumindelivery AT xuh noveltumortargetingselfassemblingpeptidenanofiberasacarrierforeffectivecurcumindelivery AT zhangy noveltumortargetingselfassemblingpeptidenanofiberasacarrierforeffectivecurcumindelivery AT chul noveltumortargetingselfassemblingpeptidenanofiberasacarrierforeffectivecurcumindelivery AT liuq noveltumortargetingselfassemblingpeptidenanofiberasacarrierforeffectivecurcumindelivery AT songn noveltumortargetingselfassemblingpeptidenanofiberasacarrierforeffectivecurcumindelivery AT yangc noveltumortargetingselfassemblingpeptidenanofiberasacarrierforeffectivecurcumindelivery |