Regulatory Ability of <i>Lactiplantibacillus plantarum</i> on Human Skin Health by Counteracting In Vitro <i>Malassezia furfur</i> Effects
The skin serves as the first barrier against pathogen attacks, thanks to its multifunctional microbial community. <i>Malassezia furfur</i> is a commensal organism of normal cutaneous microflora but is also a cause of skin diseases. It acts on different cell pattern recognition receptors...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/12/1153 |
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| author | Alessandra Fusco Brunella Perfetto Vittoria Savio Adriana Chiaromonte Giovanna Torelli Giovanna Donnarumma Adone Baroni |
| author_facet | Alessandra Fusco Brunella Perfetto Vittoria Savio Adriana Chiaromonte Giovanna Torelli Giovanna Donnarumma Adone Baroni |
| author_sort | Alessandra Fusco |
| collection | DOAJ |
| description | The skin serves as the first barrier against pathogen attacks, thanks to its multifunctional microbial community. <i>Malassezia furfur</i> is a commensal organism of normal cutaneous microflora but is also a cause of skin diseases. It acts on different cell pattern recognition receptors (TLRs, AhR, NLRP3 inflammasome) leading to cellular damage, barrier impairment, and inflammatory cytokines production. <i>Lactobacillus</i> spp. Is an endogenous inhabitant of healthy skin, and studies have proven its beneficial role in wound healing, skin inflammation, and protection against pathogen infections. The aim of our study is to demonstrate the ability of live <i>Lactiplantibacillus plantarum</i> to interfere with the harmful effects of the yeast on human keratinocytes (HaCat) in vitro. To enable this, the cells were treated with <i>M. furfur</i>, either alone or in the presence of <i>L. plantarum</i>. To study the inflammasome activation, cells require a stimulus triggering inflammation (LPS) before <i>M. furfur</i> infection, with or without <i>L. plantarum. L. plantarum</i> effectively counteracts all the harmful strategies of yeast, reducing the phospholipase activity, accelerating wound repair, restoring barrier integrity, reducing AhR and NLRP3 inflammasome activation, and, consequently, releasing inflammatory cytokines. Although lactobacilli have a long history of use in fermented foods, it can be speculated that they can also have health-promoting activities when topically applied. |
| first_indexed | 2024-03-08T20:37:02Z |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-08T20:37:02Z |
| publishDate | 2023-11-01 |
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| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-9d2d7f54a1cf4279aad575d9d111d55e2023-12-22T14:19:19ZengMDPI AGJournal of Fungi2309-608X2023-11-01912115310.3390/jof9121153Regulatory Ability of <i>Lactiplantibacillus plantarum</i> on Human Skin Health by Counteracting In Vitro <i>Malassezia furfur</i> EffectsAlessandra Fusco0Brunella Perfetto1Vittoria Savio2Adriana Chiaromonte3Giovanna Torelli4Giovanna Donnarumma5Adone Baroni6Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, Section of Microbiology and Clinical Microbiology, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, Section of Microbiology and Clinical Microbiology, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, Section of Microbiology and Clinical Microbiology, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, Section of Microbiology and Clinical Microbiology, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Experimental Medicine, Section of Microbiology and Clinical Microbiology, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyDepartment of Mental and Physical Health and Preventive Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalyThe skin serves as the first barrier against pathogen attacks, thanks to its multifunctional microbial community. <i>Malassezia furfur</i> is a commensal organism of normal cutaneous microflora but is also a cause of skin diseases. It acts on different cell pattern recognition receptors (TLRs, AhR, NLRP3 inflammasome) leading to cellular damage, barrier impairment, and inflammatory cytokines production. <i>Lactobacillus</i> spp. Is an endogenous inhabitant of healthy skin, and studies have proven its beneficial role in wound healing, skin inflammation, and protection against pathogen infections. The aim of our study is to demonstrate the ability of live <i>Lactiplantibacillus plantarum</i> to interfere with the harmful effects of the yeast on human keratinocytes (HaCat) in vitro. To enable this, the cells were treated with <i>M. furfur</i>, either alone or in the presence of <i>L. plantarum</i>. To study the inflammasome activation, cells require a stimulus triggering inflammation (LPS) before <i>M. furfur</i> infection, with or without <i>L. plantarum. L. plantarum</i> effectively counteracts all the harmful strategies of yeast, reducing the phospholipase activity, accelerating wound repair, restoring barrier integrity, reducing AhR and NLRP3 inflammasome activation, and, consequently, releasing inflammatory cytokines. Although lactobacilli have a long history of use in fermented foods, it can be speculated that they can also have health-promoting activities when topically applied.https://www.mdpi.com/2309-608X/9/12/1153<i>Malassezia furfur</i><i>Lactiplantibacillus plantarum</i>skin barrierinflammasomearyl hydrocarbon receptorNrf-2 |
| spellingShingle | Alessandra Fusco Brunella Perfetto Vittoria Savio Adriana Chiaromonte Giovanna Torelli Giovanna Donnarumma Adone Baroni Regulatory Ability of <i>Lactiplantibacillus plantarum</i> on Human Skin Health by Counteracting In Vitro <i>Malassezia furfur</i> Effects Journal of Fungi <i>Malassezia furfur</i> <i>Lactiplantibacillus plantarum</i> skin barrier inflammasome aryl hydrocarbon receptor Nrf-2 |
| title | Regulatory Ability of <i>Lactiplantibacillus plantarum</i> on Human Skin Health by Counteracting In Vitro <i>Malassezia furfur</i> Effects |
| title_full | Regulatory Ability of <i>Lactiplantibacillus plantarum</i> on Human Skin Health by Counteracting In Vitro <i>Malassezia furfur</i> Effects |
| title_fullStr | Regulatory Ability of <i>Lactiplantibacillus plantarum</i> on Human Skin Health by Counteracting In Vitro <i>Malassezia furfur</i> Effects |
| title_full_unstemmed | Regulatory Ability of <i>Lactiplantibacillus plantarum</i> on Human Skin Health by Counteracting In Vitro <i>Malassezia furfur</i> Effects |
| title_short | Regulatory Ability of <i>Lactiplantibacillus plantarum</i> on Human Skin Health by Counteracting In Vitro <i>Malassezia furfur</i> Effects |
| title_sort | regulatory ability of i lactiplantibacillus plantarum i on human skin health by counteracting in vitro i malassezia furfur i effects |
| topic | <i>Malassezia furfur</i> <i>Lactiplantibacillus plantarum</i> skin barrier inflammasome aryl hydrocarbon receptor Nrf-2 |
| url | https://www.mdpi.com/2309-608X/9/12/1153 |
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