Nanoparticles of Chitosan Loaded Ciprofloxacin: Fabrication and Antimicrobial Activity
Purpose: Chitosan is a natural mucoadhesive polymer with antibacterial activity. In the present study, chitosan (CS) nanoparticles were investigated as a vehicle for delivery of antibiotic, ciprofloxacin hydrochloride. Methods: Ionotropic gelation method was used for preparation chitosan nanoparticl...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tabriz University of Medical Sciences
2017-09-01
|
| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-7-427.pdf |
| Summary: | Purpose: Chitosan is a natural
mucoadhesive polymer with antibacterial activity. In the present study,
chitosan (CS) nanoparticles were investigated as a vehicle for delivery of
antibiotic, ciprofloxacin hydrochloride.
Methods: Ionotropic gelation method was
used for preparation chitosan nanoparticles. The effects of various factors
including concentration of CS, concentration of tripolyphosphate (TPP), and
homogenization rate on the size of nanoparticles were studied. The effects of
various mass ratios of CS to ciprofloxacin hydrochloride on the encapsulation
efficiency of nanoparticles were assessed.
Results: The particles prepared under optimal condition of
0.45% CS concentration, 0.45% TPP concentration and homogenizer rate at 6000
rpm, had 72 nm diameter. In these particles with 1:0.5 mass ratio of CS to
ciprofloxacin hydrochloride, the encapsulation efficiency was 23%. The
antibacterial activity of chitosan nanoparticles and ciprofloxacin-loaded
nanoparticles against E.coli and S.aureus was evaluated by
calculation of minimum inhibitory concentration (MIC). Results showed that MIC
of ciprofloxacin loaded chitosan nanoparticles was 50% lower than that of
ciprofloxacin hydrochloride alone in both of microorganism species.
Nanoparticles without drug exhibited antibacterial activity at higher
concentrations and MIC of them against E.coli and S.aureus was
177 and 277 µg/ml, respectively.
Conclusion: Therefore chitosan
nanoparticles could be applied as carrier for decreasing the dose of antibacterial
agents in the infections. |
|---|---|
| ISSN: | 2228-5881 2251-7308 |