Nanoparticles of Chitosan Loaded Ciprofloxacin: Fabrication and Antimicrobial Activity
Purpose: Chitosan is a natural mucoadhesive polymer with antibacterial activity. In the present study, chitosan (CS) nanoparticles were investigated as a vehicle for delivery of antibiotic, ciprofloxacin hydrochloride. Methods: Ionotropic gelation method was used for preparation chitosan nanoparticl...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2017-09-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-7-427.pdf |
| _version_ | 1811318018724069376 |
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| author | Zahra Sobhani Soliman Mohammadi Samani Hashem Montaseri Elham Khezri |
| author_facet | Zahra Sobhani Soliman Mohammadi Samani Hashem Montaseri Elham Khezri |
| author_sort | Zahra Sobhani |
| collection | DOAJ |
| description | Purpose: Chitosan is a natural
mucoadhesive polymer with antibacterial activity. In the present study,
chitosan (CS) nanoparticles were investigated as a vehicle for delivery of
antibiotic, ciprofloxacin hydrochloride.
Methods: Ionotropic gelation method was
used for preparation chitosan nanoparticles. The effects of various factors
including concentration of CS, concentration of tripolyphosphate (TPP), and
homogenization rate on the size of nanoparticles were studied. The effects of
various mass ratios of CS to ciprofloxacin hydrochloride on the encapsulation
efficiency of nanoparticles were assessed.
Results: The particles prepared under optimal condition of
0.45% CS concentration, 0.45% TPP concentration and homogenizer rate at 6000
rpm, had 72 nm diameter. In these particles with 1:0.5 mass ratio of CS to
ciprofloxacin hydrochloride, the encapsulation efficiency was 23%. The
antibacterial activity of chitosan nanoparticles and ciprofloxacin-loaded
nanoparticles against E.coli and S.aureus was evaluated by
calculation of minimum inhibitory concentration (MIC). Results showed that MIC
of ciprofloxacin loaded chitosan nanoparticles was 50% lower than that of
ciprofloxacin hydrochloride alone in both of microorganism species.
Nanoparticles without drug exhibited antibacterial activity at higher
concentrations and MIC of them against E.coli and S.aureus was
177 and 277 µg/ml, respectively.
Conclusion: Therefore chitosan
nanoparticles could be applied as carrier for decreasing the dose of antibacterial
agents in the infections. |
| first_indexed | 2024-04-13T12:18:42Z |
| format | Article |
| id | doaj.art-9d2e4da955dc40cab2c95ff303e0cc0c |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-04-13T12:18:42Z |
| publishDate | 2017-09-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-9d2e4da955dc40cab2c95ff303e0cc0c2022-12-22T02:47:17ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082017-09-017342743210.15171/apb.2017.051APB_17108_20170128215430Nanoparticles of Chitosan Loaded Ciprofloxacin: Fabrication and Antimicrobial ActivityZahra Sobhani0Soliman Mohammadi Samani1Hashem Montaseri2Elham Khezri3Department of quality control, Faculty of pharmacy, Shiraz University of Medical Science, Shiraz, Iran.Department of pharmaceutics, Faculty of pharmacy, Shiraz University of Medical Science, Shiraz, Iran.Department of quality control, Faculty of pharmacy, Shiraz University of Medical Science, Shiraz, Iran.Department of quality control, Faculty of pharmacy, Shiraz University of Medical Science, Shiraz, Iran.Purpose: Chitosan is a natural mucoadhesive polymer with antibacterial activity. In the present study, chitosan (CS) nanoparticles were investigated as a vehicle for delivery of antibiotic, ciprofloxacin hydrochloride. Methods: Ionotropic gelation method was used for preparation chitosan nanoparticles. The effects of various factors including concentration of CS, concentration of tripolyphosphate (TPP), and homogenization rate on the size of nanoparticles were studied. The effects of various mass ratios of CS to ciprofloxacin hydrochloride on the encapsulation efficiency of nanoparticles were assessed. Results: The particles prepared under optimal condition of 0.45% CS concentration, 0.45% TPP concentration and homogenizer rate at 6000 rpm, had 72 nm diameter. In these particles with 1:0.5 mass ratio of CS to ciprofloxacin hydrochloride, the encapsulation efficiency was 23%. The antibacterial activity of chitosan nanoparticles and ciprofloxacin-loaded nanoparticles against E.coli and S.aureus was evaluated by calculation of minimum inhibitory concentration (MIC). Results showed that MIC of ciprofloxacin loaded chitosan nanoparticles was 50% lower than that of ciprofloxacin hydrochloride alone in both of microorganism species. Nanoparticles without drug exhibited antibacterial activity at higher concentrations and MIC of them against E.coli and S.aureus was 177 and 277 µg/ml, respectively. Conclusion: Therefore chitosan nanoparticles could be applied as carrier for decreasing the dose of antibacterial agents in the infections.http://journals.tbzmed.ac.ir/APB/Manuscript/APB-7-427.pdfChitosanCiprofloxacinNanoparticleTripolyphosphate |
| spellingShingle | Zahra Sobhani Soliman Mohammadi Samani Hashem Montaseri Elham Khezri Nanoparticles of Chitosan Loaded Ciprofloxacin: Fabrication and Antimicrobial Activity Advanced Pharmaceutical Bulletin Chitosan Ciprofloxacin Nanoparticle Tripolyphosphate |
| title | Nanoparticles of Chitosan Loaded Ciprofloxacin: Fabrication and
Antimicrobial Activity |
| title_full | Nanoparticles of Chitosan Loaded Ciprofloxacin: Fabrication and
Antimicrobial Activity |
| title_fullStr | Nanoparticles of Chitosan Loaded Ciprofloxacin: Fabrication and
Antimicrobial Activity |
| title_full_unstemmed | Nanoparticles of Chitosan Loaded Ciprofloxacin: Fabrication and
Antimicrobial Activity |
| title_short | Nanoparticles of Chitosan Loaded Ciprofloxacin: Fabrication and
Antimicrobial Activity |
| title_sort | nanoparticles of chitosan loaded ciprofloxacin fabrication and antimicrobial activity |
| topic | Chitosan Ciprofloxacin Nanoparticle Tripolyphosphate |
| url | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-7-427.pdf |
| work_keys_str_mv | AT zahrasobhani nanoparticlesofchitosanloadedciprofloxacinfabricationandantimicrobialactivity AT solimanmohammadisamani nanoparticlesofchitosanloadedciprofloxacinfabricationandantimicrobialactivity AT hashemmontaseri nanoparticlesofchitosanloadedciprofloxacinfabricationandantimicrobialactivity AT elhamkhezri nanoparticlesofchitosanloadedciprofloxacinfabricationandantimicrobialactivity |