Oligohexamethylene Guanidine Derivative as a Means to Prevent Biological Fouling of a Polymer-Based Composite Optical Oxygen Sensor
The use of biocidal agents is a common practice for protection against biofouling in biomass-rich environments. In this paper, oligohexamethyleneguanidine (OHMG) polymer, known for its biocidal properties, was further modified with para-aminosalicylic acid (PAS) to enhance its properties against mic...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/23/4508 |
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| author | Maxim D. Lisowski Elizaveta V. Korobova Alina O. Naumova Igor P. Sedishev Alina A. Markova Minh Tuan Nguyen Vladimir A. Kuzmin Artemiy I. Nichugovskiy Vyacheslav A. Arlyapov Nikolay A. Yashtulov Pavel V. Melnikov |
| author_facet | Maxim D. Lisowski Elizaveta V. Korobova Alina O. Naumova Igor P. Sedishev Alina A. Markova Minh Tuan Nguyen Vladimir A. Kuzmin Artemiy I. Nichugovskiy Vyacheslav A. Arlyapov Nikolay A. Yashtulov Pavel V. Melnikov |
| author_sort | Maxim D. Lisowski |
| collection | DOAJ |
| description | The use of biocidal agents is a common practice for protection against biofouling in biomass-rich environments. In this paper, oligohexamethyleneguanidine (OHMG) polymer, known for its biocidal properties, was further modified with para-aminosalicylic acid (PAS) to enhance its properties against microorganisms coated with a lipid membrane. The structure of the product was confirmed by <sup>1</sup>H NMR, <sup>13</sup>C NMR, and FTIR spectroscopy. The values of the minimum inhibitory concentration (MIC) against <i>Mycobacterium smegmatis</i> ATCC 607 and <i>Pseudomonas chlororaphis</i> 449 were found to be 1.40 and 1.05 μg/mL, respectively. The synthesized substance was used as an additive to the polymer matrix of the composite optical oxygen sensor material. A series of samples with different contents of OHMG-PAS was prepared using a co-dissolution method implying the fabrication of a coating from a solution containing both polymers. It turned out that the mutual influence of the components significantly affects the distribution of the indicator in the matrix, surface morphology, and contact angle. The optimal polymer content turned out to be wt.3%, at which point the water contact angle reaches almost 122°, and the fouling rate decreases by almost five times, which is confirmed by both the respiratory MTT assay and confocal microscopy with staining. This opens up prospects for creating stable and biofouling-resistant sensor elements for use in air tanks or seawater. |
| first_indexed | 2024-03-09T01:44:14Z |
| format | Article |
| id | doaj.art-9d2ec526a1cb49b6aef5cf6bace298a7 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T01:44:14Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-9d2ec526a1cb49b6aef5cf6bace298a72023-12-08T15:24:14ZengMDPI AGPolymers2073-43602023-11-011523450810.3390/polym15234508Oligohexamethylene Guanidine Derivative as a Means to Prevent Biological Fouling of a Polymer-Based Composite Optical Oxygen SensorMaxim D. Lisowski0Elizaveta V. Korobova1Alina O. Naumova2Igor P. Sedishev3Alina A. Markova4Minh Tuan Nguyen5Vladimir A. Kuzmin6Artemiy I. Nichugovskiy7Vyacheslav A. Arlyapov8Nikolay A. Yashtulov9Pavel V. Melnikov10M.V. Lomonosov Institute of Fine Chemical Technologies, MIREA—Russian Technological University, 119571 Moscow, RussiaM.V. Lomonosov Institute of Fine Chemical Technologies, MIREA—Russian Technological University, 119571 Moscow, RussiaM.V. Lomonosov Institute of Fine Chemical Technologies, MIREA—Russian Technological University, 119571 Moscow, RussiaM.V. Lomonosov Institute of Fine Chemical Technologies, MIREA—Russian Technological University, 119571 Moscow, RussiaEmanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygin Street, 119334 Moscow, RussiaEmanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygin Street, 119334 Moscow, RussiaEmanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygin Street, 119334 Moscow, RussiaM.V. Lomonosov Institute of Fine Chemical Technologies, MIREA—Russian Technological University, 119571 Moscow, RussiaResearch Center “BioChemTech”, Tula State University, 92 Lenin Avenue, 300012 Tula, RussiaM.V. Lomonosov Institute of Fine Chemical Technologies, MIREA—Russian Technological University, 119571 Moscow, RussiaM.V. Lomonosov Institute of Fine Chemical Technologies, MIREA—Russian Technological University, 119571 Moscow, RussiaThe use of biocidal agents is a common practice for protection against biofouling in biomass-rich environments. In this paper, oligohexamethyleneguanidine (OHMG) polymer, known for its biocidal properties, was further modified with para-aminosalicylic acid (PAS) to enhance its properties against microorganisms coated with a lipid membrane. The structure of the product was confirmed by <sup>1</sup>H NMR, <sup>13</sup>C NMR, and FTIR spectroscopy. The values of the minimum inhibitory concentration (MIC) against <i>Mycobacterium smegmatis</i> ATCC 607 and <i>Pseudomonas chlororaphis</i> 449 were found to be 1.40 and 1.05 μg/mL, respectively. The synthesized substance was used as an additive to the polymer matrix of the composite optical oxygen sensor material. A series of samples with different contents of OHMG-PAS was prepared using a co-dissolution method implying the fabrication of a coating from a solution containing both polymers. It turned out that the mutual influence of the components significantly affects the distribution of the indicator in the matrix, surface morphology, and contact angle. The optimal polymer content turned out to be wt.3%, at which point the water contact angle reaches almost 122°, and the fouling rate decreases by almost five times, which is confirmed by both the respiratory MTT assay and confocal microscopy with staining. This opens up prospects for creating stable and biofouling-resistant sensor elements for use in air tanks or seawater.https://www.mdpi.com/2073-4360/15/23/4508oxygen sensoroptical sensorantimicrobial propertiesoligohexamethyleneguanidinenanostructured surfacesurface modification |
| spellingShingle | Maxim D. Lisowski Elizaveta V. Korobova Alina O. Naumova Igor P. Sedishev Alina A. Markova Minh Tuan Nguyen Vladimir A. Kuzmin Artemiy I. Nichugovskiy Vyacheslav A. Arlyapov Nikolay A. Yashtulov Pavel V. Melnikov Oligohexamethylene Guanidine Derivative as a Means to Prevent Biological Fouling of a Polymer-Based Composite Optical Oxygen Sensor Polymers oxygen sensor optical sensor antimicrobial properties oligohexamethyleneguanidine nanostructured surface surface modification |
| title | Oligohexamethylene Guanidine Derivative as a Means to Prevent Biological Fouling of a Polymer-Based Composite Optical Oxygen Sensor |
| title_full | Oligohexamethylene Guanidine Derivative as a Means to Prevent Biological Fouling of a Polymer-Based Composite Optical Oxygen Sensor |
| title_fullStr | Oligohexamethylene Guanidine Derivative as a Means to Prevent Biological Fouling of a Polymer-Based Composite Optical Oxygen Sensor |
| title_full_unstemmed | Oligohexamethylene Guanidine Derivative as a Means to Prevent Biological Fouling of a Polymer-Based Composite Optical Oxygen Sensor |
| title_short | Oligohexamethylene Guanidine Derivative as a Means to Prevent Biological Fouling of a Polymer-Based Composite Optical Oxygen Sensor |
| title_sort | oligohexamethylene guanidine derivative as a means to prevent biological fouling of a polymer based composite optical oxygen sensor |
| topic | oxygen sensor optical sensor antimicrobial properties oligohexamethyleneguanidine nanostructured surface surface modification |
| url | https://www.mdpi.com/2073-4360/15/23/4508 |
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