Effects of husbandry systems and Chinese indigenous chicken strain on cecum microbial diversity
Objective This study was to evaluate the effect of husbandry systems and strains on cecum microbial diversity of Jingyang chickens under the same dietary conditions. Methods A total of 320 laying hens (body weight, 1.70±0.15 kg; 47 weeks old) were randomly allocated to one of the four treatments: i)...
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Asian-Australasian Association of Animal Production Societies
2020-10-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
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| Online Access: | http://www.ajas.info/upload/pdf/ajas-19-0157.pdf |
| _version_ | 1818276654746697728 |
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| author | Xiuxue Dong Bing Hu Wenlong Wan Yanzhang Gong Yanping Feng |
| author_facet | Xiuxue Dong Bing Hu Wenlong Wan Yanzhang Gong Yanping Feng |
| author_sort | Xiuxue Dong |
| collection | DOAJ |
| description | Objective This study was to evaluate the effect of husbandry systems and strains on cecum microbial diversity of Jingyang chickens under the same dietary conditions. Methods A total of 320 laying hens (body weight, 1.70±0.15 kg; 47 weeks old) were randomly allocated to one of the four treatments: i) Silver-feathered hens in enrichment cages (SEC) with an individual cage (70×60×75 cm), ii) Silver-feathered hens in free range (SFR) with the stocking density of 1.5 chickens per ten square meters, iii) Gold-feathered hens in enrichment cages (GEC), iv) Gold-feathered hens in free range (GFR). The experiment lasted 8 weeks and the cecum fecal samples were collected for 16S rDNA high throughput sequencing at the end of experiment. Results i) The core microbiota was composed of Bacteroidetes (49% to 60%), Firmicutes (21% to 32%) and Proteobacteria (2% to 4%) at the phylum level. ii) The core bacteria were Bacteroides (26% to 31%), Rikenellaceae (9% to 16%), Parabacteroides (2% to 5%) and Lachnoclostridium (2% to 6%) at the genus level. iii) The indexes of operational taxonomic unit, Shannon, Simpson and observed species were all higher in SFR group than in SEC group while in GEC group than in GFR group, with SFR group showing the greatest diversity of cecum microorganisms among the four groups. iv) The clustering result was consistent with the strain classification, with a similar composition of cecum bacteria in the two strains of laying hens. Conclusion The core microbiota were not altered by husbandry systems or strains. The free-range system increased the diversity of cecal microbes only for silver feathered hens. However, the cecum microbial composition was similar in two strain treatments under the same dietary conditions. |
| first_indexed | 2024-12-12T22:49:05Z |
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| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-12T22:49:05Z |
| publishDate | 2020-10-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-9d2f3b5f7e0340d793e918f2f5c614232022-12-22T00:09:07ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172020-10-0133101610161610.5713/ajas.19.015724400Effects of husbandry systems and Chinese indigenous chicken strain on cecum microbial diversityXiuxue Dong0Bing Hu1Wenlong Wan2Yanzhang Gong3Yanping Feng4 College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaObjective This study was to evaluate the effect of husbandry systems and strains on cecum microbial diversity of Jingyang chickens under the same dietary conditions. Methods A total of 320 laying hens (body weight, 1.70±0.15 kg; 47 weeks old) were randomly allocated to one of the four treatments: i) Silver-feathered hens in enrichment cages (SEC) with an individual cage (70×60×75 cm), ii) Silver-feathered hens in free range (SFR) with the stocking density of 1.5 chickens per ten square meters, iii) Gold-feathered hens in enrichment cages (GEC), iv) Gold-feathered hens in free range (GFR). The experiment lasted 8 weeks and the cecum fecal samples were collected for 16S rDNA high throughput sequencing at the end of experiment. Results i) The core microbiota was composed of Bacteroidetes (49% to 60%), Firmicutes (21% to 32%) and Proteobacteria (2% to 4%) at the phylum level. ii) The core bacteria were Bacteroides (26% to 31%), Rikenellaceae (9% to 16%), Parabacteroides (2% to 5%) and Lachnoclostridium (2% to 6%) at the genus level. iii) The indexes of operational taxonomic unit, Shannon, Simpson and observed species were all higher in SFR group than in SEC group while in GEC group than in GFR group, with SFR group showing the greatest diversity of cecum microorganisms among the four groups. iv) The clustering result was consistent with the strain classification, with a similar composition of cecum bacteria in the two strains of laying hens. Conclusion The core microbiota were not altered by husbandry systems or strains. The free-range system increased the diversity of cecal microbes only for silver feathered hens. However, the cecum microbial composition was similar in two strain treatments under the same dietary conditions.http://www.ajas.info/upload/pdf/ajas-19-0157.pdfjingyang chickenhusbandry systemcecum microbiomediversityillumina miseq sequencing |
| spellingShingle | Xiuxue Dong Bing Hu Wenlong Wan Yanzhang Gong Yanping Feng Effects of husbandry systems and Chinese indigenous chicken strain on cecum microbial diversity Asian-Australasian Journal of Animal Sciences jingyang chicken husbandry system cecum microbiome diversity illumina miseq sequencing |
| title | Effects of husbandry systems and Chinese indigenous chicken strain on cecum microbial diversity |
| title_full | Effects of husbandry systems and Chinese indigenous chicken strain on cecum microbial diversity |
| title_fullStr | Effects of husbandry systems and Chinese indigenous chicken strain on cecum microbial diversity |
| title_full_unstemmed | Effects of husbandry systems and Chinese indigenous chicken strain on cecum microbial diversity |
| title_short | Effects of husbandry systems and Chinese indigenous chicken strain on cecum microbial diversity |
| title_sort | effects of husbandry systems and chinese indigenous chicken strain on cecum microbial diversity |
| topic | jingyang chicken husbandry system cecum microbiome diversity illumina miseq sequencing |
| url | http://www.ajas.info/upload/pdf/ajas-19-0157.pdf |
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