Determination of genome size variation among varieties of Ilex cornuta (Aquifoliaceae) by fow cytometry
Ilex cornuta Lindl. & Paxton is a commercially important horticultural species worldwide, and extensive cultivation and hybridization have produced many varieties. Despite the considerable breeding, selection, widespread cultivation and domestication, which may have a significant role in the com...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Firenze University Press
2023-04-01
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| Series: | Caryologia |
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| Online Access: | https://riviste.fupress.net/index.php/caryologia/article/view/1902 |
| _version_ | 1797833579471831040 |
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| author | Peng Zhou Jiao Li Jing Huang Fei Li Qiang Zhang Min Zhang |
| author_facet | Peng Zhou Jiao Li Jing Huang Fei Li Qiang Zhang Min Zhang |
| author_sort | Peng Zhou |
| collection | DOAJ |
| description | Ilex cornuta Lindl. & Paxton is a commercially important horticultural species worldwide, and extensive cultivation and hybridization have produced many varieties. Despite the considerable breeding, selection, widespread cultivation and domestication, which may have a significant role in the composition of genomes, there are no other previous reports of intraspecific genome size variation in the different cultivars or hybrids of this species. In the present work, genome size of 12 varieties of I. cornuta was assessed and analyzed through high-resolution flow cytometry (FCM). Nuclear DNA was analyzed using nuclei isolated from young leaves, which used propidium iodide (PI) staining, with rice (Oryza sativa cv. Nipponbare) as internal reference. As a result, statistically significant differences in genome size were detected among all diploid I. cornuta varieties considered. The estimated genome size (2C value) of I. cornuta varieties ranged from 1.47 to 1.80 pg, with 1.22-fold variation and an average size of 1.65 pg. The domestication and interspecific hybridization induced variation of genome size in I. cornuta, and the genome size of hybrids exhibited a wider range of variation compared with that of cultivars. In summary, flow cytometry is a useful tool to analyze the genome size of I. cornuta. The first report of the genome sizes of varieties of this species would provide useful data for further research on I. cornuta, and enrich the C value database of Ilex L. What’s more, our findings could be the foundation in the future of I. cornuta genome sequencing and breeding programs.
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| first_indexed | 2024-04-09T14:26:17Z |
| format | Article |
| id | doaj.art-9d363e4f56454c43a0349f8ffa98befa |
| institution | Directory Open Access Journal |
| issn | 0008-7114 2165-5391 |
| language | English |
| last_indexed | 2024-04-09T14:26:17Z |
| publishDate | 2023-04-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Caryologia |
| spelling | doaj.art-9d363e4f56454c43a0349f8ffa98befa2023-05-04T04:51:12ZengFirenze University PressCaryologia0008-71142165-53912023-04-0175410.36253/caryologia-1902Determination of genome size variation among varieties of Ilex cornuta (Aquifoliaceae) by fow cytometryPeng Zhou0Jiao Li1Jing Huang2Fei Li3Qiang Zhang4Min Zhang5Jiangsu Academy of Forestry, Nanjing 211153, JiangsuCo‑Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of State Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, JiangsuJiangsu Academy of Forestry, Nanjing 211153, JiangsuJiangsu Academy of Forestry, Nanjing 211153, JiangsuCo‑Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of State Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, JiangsuJiangsu Academy of Forestry, Nanjing 211153, JiangsuIlex cornuta Lindl. & Paxton is a commercially important horticultural species worldwide, and extensive cultivation and hybridization have produced many varieties. Despite the considerable breeding, selection, widespread cultivation and domestication, which may have a significant role in the composition of genomes, there are no other previous reports of intraspecific genome size variation in the different cultivars or hybrids of this species. In the present work, genome size of 12 varieties of I. cornuta was assessed and analyzed through high-resolution flow cytometry (FCM). Nuclear DNA was analyzed using nuclei isolated from young leaves, which used propidium iodide (PI) staining, with rice (Oryza sativa cv. Nipponbare) as internal reference. As a result, statistically significant differences in genome size were detected among all diploid I. cornuta varieties considered. The estimated genome size (2C value) of I. cornuta varieties ranged from 1.47 to 1.80 pg, with 1.22-fold variation and an average size of 1.65 pg. The domestication and interspecific hybridization induced variation of genome size in I. cornuta, and the genome size of hybrids exhibited a wider range of variation compared with that of cultivars. In summary, flow cytometry is a useful tool to analyze the genome size of I. cornuta. The first report of the genome sizes of varieties of this species would provide useful data for further research on I. cornuta, and enrich the C value database of Ilex L. What’s more, our findings could be the foundation in the future of I. cornuta genome sequencing and breeding programs. https://riviste.fupress.net/index.php/caryologia/article/view/1902DNA contentFlow cytometryHybridsCultivarsIlex cornutaGenome size |
| spellingShingle | Peng Zhou Jiao Li Jing Huang Fei Li Qiang Zhang Min Zhang Determination of genome size variation among varieties of Ilex cornuta (Aquifoliaceae) by fow cytometry Caryologia DNA content Flow cytometry Hybrids Cultivars Ilex cornuta Genome size |
| title | Determination of genome size variation among varieties of Ilex cornuta (Aquifoliaceae) by fow cytometry |
| title_full | Determination of genome size variation among varieties of Ilex cornuta (Aquifoliaceae) by fow cytometry |
| title_fullStr | Determination of genome size variation among varieties of Ilex cornuta (Aquifoliaceae) by fow cytometry |
| title_full_unstemmed | Determination of genome size variation among varieties of Ilex cornuta (Aquifoliaceae) by fow cytometry |
| title_short | Determination of genome size variation among varieties of Ilex cornuta (Aquifoliaceae) by fow cytometry |
| title_sort | determination of genome size variation among varieties of ilex cornuta aquifoliaceae by fow cytometry |
| topic | DNA content Flow cytometry Hybrids Cultivars Ilex cornuta Genome size |
| url | https://riviste.fupress.net/index.php/caryologia/article/view/1902 |
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