H-rev107 Regulates Cytochrome P450 Reductase Activity and Increases Lipid Accumulation.
H-rev107 is a member of the HREV107 type II tumor suppressor gene family and acts as a phospholipase to catalyze the release of fatty acids from glycerophospholipid. H-rev107 has been shown to play an important role in fat metabolism in adipocytes through the PGE2/cAMP pathway, but the detailed mole...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4575093?pdf=render |
| _version_ | 1818129762532458496 |
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| author | Fu-Ming Tsai Mao-Liang Chen Lu-Kai Wang Ming-Cheng Lee |
| author_facet | Fu-Ming Tsai Mao-Liang Chen Lu-Kai Wang Ming-Cheng Lee |
| author_sort | Fu-Ming Tsai |
| collection | DOAJ |
| description | H-rev107 is a member of the HREV107 type II tumor suppressor gene family and acts as a phospholipase to catalyze the release of fatty acids from glycerophospholipid. H-rev107 has been shown to play an important role in fat metabolism in adipocytes through the PGE2/cAMP pathway, but the detailed molecular mechanism underlying H-rev107-mediated lipid degradation has not been studied. In this study, the interaction between H-rev107 and cytochrome P450 reductase (POR), which is involved in hepatic lipid content regulation, was determined by yeast two-hybrid screen and confirmed by using in vitro pull down assays and immunofluorescent staining. The expression of POR in H-rev107-expressing cells enhanced the H-rev107-mediated release of arachidonic acid. However, H-rev107 inhibited POR activity and relieved POR-mediated decreased triglyceride content in HtTA and HeLa cervical cells. The inhibitory effect of H-rev107 will be abolished when POR-expressing cells transfected with PLA2-lacking pH-rev107 or treated with PLA2 inhibitor. Silencing of H-rev107 using siRNA resulted in increased glycerol production and reversion of free fatty acid-mediated growth suppression in Huh7 hepatic cells. In summary, our results revealed that H-rev107 is also involved in lipid accumulation in liver cells through the POR pathway via its PLA2 activity. |
| first_indexed | 2024-12-11T07:54:18Z |
| format | Article |
| id | doaj.art-9d389cd7bd6f4b7fa87c9b3393738cd9 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T07:54:18Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9d389cd7bd6f4b7fa87c9b3393738cd92022-12-22T01:15:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013858610.1371/journal.pone.0138586H-rev107 Regulates Cytochrome P450 Reductase Activity and Increases Lipid Accumulation.Fu-Ming TsaiMao-Liang ChenLu-Kai WangMing-Cheng LeeH-rev107 is a member of the HREV107 type II tumor suppressor gene family and acts as a phospholipase to catalyze the release of fatty acids from glycerophospholipid. H-rev107 has been shown to play an important role in fat metabolism in adipocytes through the PGE2/cAMP pathway, but the detailed molecular mechanism underlying H-rev107-mediated lipid degradation has not been studied. In this study, the interaction between H-rev107 and cytochrome P450 reductase (POR), which is involved in hepatic lipid content regulation, was determined by yeast two-hybrid screen and confirmed by using in vitro pull down assays and immunofluorescent staining. The expression of POR in H-rev107-expressing cells enhanced the H-rev107-mediated release of arachidonic acid. However, H-rev107 inhibited POR activity and relieved POR-mediated decreased triglyceride content in HtTA and HeLa cervical cells. The inhibitory effect of H-rev107 will be abolished when POR-expressing cells transfected with PLA2-lacking pH-rev107 or treated with PLA2 inhibitor. Silencing of H-rev107 using siRNA resulted in increased glycerol production and reversion of free fatty acid-mediated growth suppression in Huh7 hepatic cells. In summary, our results revealed that H-rev107 is also involved in lipid accumulation in liver cells through the POR pathway via its PLA2 activity.http://europepmc.org/articles/PMC4575093?pdf=render |
| spellingShingle | Fu-Ming Tsai Mao-Liang Chen Lu-Kai Wang Ming-Cheng Lee H-rev107 Regulates Cytochrome P450 Reductase Activity and Increases Lipid Accumulation. PLoS ONE |
| title | H-rev107 Regulates Cytochrome P450 Reductase Activity and Increases Lipid Accumulation. |
| title_full | H-rev107 Regulates Cytochrome P450 Reductase Activity and Increases Lipid Accumulation. |
| title_fullStr | H-rev107 Regulates Cytochrome P450 Reductase Activity and Increases Lipid Accumulation. |
| title_full_unstemmed | H-rev107 Regulates Cytochrome P450 Reductase Activity and Increases Lipid Accumulation. |
| title_short | H-rev107 Regulates Cytochrome P450 Reductase Activity and Increases Lipid Accumulation. |
| title_sort | h rev107 regulates cytochrome p450 reductase activity and increases lipid accumulation |
| url | http://europepmc.org/articles/PMC4575093?pdf=render |
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