Curcumin and Vitamin C Attenuate Gentamicin-Induced Nephrotoxicity by Modulating Distinctive Reactive Species
Gentamicin remains widely used in all age groups despite its well-documented nephrotoxicity; however, no adjuvant therapies have been established to counteract this side effect. Our study aimed to experimentally determine whether curcumin and vitamin C have nephroprotective effects and whether certa...
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2218-1989/13/1/49 |
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| author | Anamaria Magdalena Tomşa Andreea Liana Răchişan Stanca Lucia Pandrea Andreea Benea Ana Uifălean Corina Toma Roxana Popa Alina Elena Pârvu Lia Monica Junie |
| author_facet | Anamaria Magdalena Tomşa Andreea Liana Răchişan Stanca Lucia Pandrea Andreea Benea Ana Uifălean Corina Toma Roxana Popa Alina Elena Pârvu Lia Monica Junie |
| author_sort | Anamaria Magdalena Tomşa |
| collection | DOAJ |
| description | Gentamicin remains widely used in all age groups despite its well-documented nephrotoxicity; however, no adjuvant therapies have been established to counteract this side effect. Our study aimed to experimentally determine whether curcumin and vitamin C have nephroprotective effects and whether certain reactive species could be used as markers of early gentamicin nephrotoxicity. Wistar adult male rats were evenly distributed into four groups: control, gentamicin, curcumin and gentamicin, vitamin C and gentamicin (gentamicin: 60 mg/kg/day, intraperitoneally, 7 days). We determined renal function (urea, creatinine), oxidative stress (malondialdehyde, nitric oxide, 3-nitrotyrosine, total oxidative stress), and antioxidant and anti-inflammatory status (thiols, total antioxidant capacity, interleukin-10). Nephrotoxicity was successfully induced, as shown by the elevated creatinine levels in the gentamicin group. In contrast, supplementation with curcumin and vitamin C prevented an increase in urea levels while decreasing total oxidative stress levels compared to the gentamicin group. Moreover, vitamin C and curcumin distinctively modulate the levels of nitric oxide and malondialdehyde. Histological analysis showed more discrete lesions in rats that received vitamin C compared to the curcumin group. |
| first_indexed | 2024-03-09T11:43:43Z |
| format | Article |
| id | doaj.art-9d3fac56331b4d46990ace350f014e91 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T11:43:43Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-9d3fac56331b4d46990ace350f014e912023-11-30T23:28:07ZengMDPI AGMetabolites2218-19892022-12-011314910.3390/metabo13010049Curcumin and Vitamin C Attenuate Gentamicin-Induced Nephrotoxicity by Modulating Distinctive Reactive SpeciesAnamaria Magdalena Tomşa0Andreea Liana Răchişan1Stanca Lucia Pandrea2Andreea Benea3Ana Uifălean4Corina Toma5Roxana Popa6Alina Elena Pârvu7Lia Monica Junie82nd Pediatrics Clinic, ‘Iuliu Hatieganu’ University of Medicine and Pharmacy, 400177 Cluj-Napoca, Romania2nd Pediatrics Clinic, ‘Iuliu Hatieganu’ University of Medicine and Pharmacy, 400177 Cluj-Napoca, RomaniaDepartment of Microbiology, ‘Iuliu Hatieganu’ University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania‘Prof. Dr. Octavian Fodor’ Regional Institute of Gastroenterology and Hepatology, 400162 Cluj-Napoca, RomaniaDepartment of Pathophysiology, ‘Iuliu Hatieganu’ University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaDepartment of Veterinary Pathology, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, RomaniaDepartment of Veterinary Pathology, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, RomaniaDepartment of Pathophysiology, ‘Iuliu Hatieganu’ University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaDepartment of Microbiology, ‘Iuliu Hatieganu’ University of Medicine and Pharmacy, 400012 Cluj-Napoca, RomaniaGentamicin remains widely used in all age groups despite its well-documented nephrotoxicity; however, no adjuvant therapies have been established to counteract this side effect. Our study aimed to experimentally determine whether curcumin and vitamin C have nephroprotective effects and whether certain reactive species could be used as markers of early gentamicin nephrotoxicity. Wistar adult male rats were evenly distributed into four groups: control, gentamicin, curcumin and gentamicin, vitamin C and gentamicin (gentamicin: 60 mg/kg/day, intraperitoneally, 7 days). We determined renal function (urea, creatinine), oxidative stress (malondialdehyde, nitric oxide, 3-nitrotyrosine, total oxidative stress), and antioxidant and anti-inflammatory status (thiols, total antioxidant capacity, interleukin-10). Nephrotoxicity was successfully induced, as shown by the elevated creatinine levels in the gentamicin group. In contrast, supplementation with curcumin and vitamin C prevented an increase in urea levels while decreasing total oxidative stress levels compared to the gentamicin group. Moreover, vitamin C and curcumin distinctively modulate the levels of nitric oxide and malondialdehyde. Histological analysis showed more discrete lesions in rats that received vitamin C compared to the curcumin group.https://www.mdpi.com/2218-1989/13/1/49gentamicin nephrotoxicityoxidative stressantioxidantlipid peroxidationmalondialdehydenitric oxide |
| spellingShingle | Anamaria Magdalena Tomşa Andreea Liana Răchişan Stanca Lucia Pandrea Andreea Benea Ana Uifălean Corina Toma Roxana Popa Alina Elena Pârvu Lia Monica Junie Curcumin and Vitamin C Attenuate Gentamicin-Induced Nephrotoxicity by Modulating Distinctive Reactive Species Metabolites gentamicin nephrotoxicity oxidative stress antioxidant lipid peroxidation malondialdehyde nitric oxide |
| title | Curcumin and Vitamin C Attenuate Gentamicin-Induced Nephrotoxicity by Modulating Distinctive Reactive Species |
| title_full | Curcumin and Vitamin C Attenuate Gentamicin-Induced Nephrotoxicity by Modulating Distinctive Reactive Species |
| title_fullStr | Curcumin and Vitamin C Attenuate Gentamicin-Induced Nephrotoxicity by Modulating Distinctive Reactive Species |
| title_full_unstemmed | Curcumin and Vitamin C Attenuate Gentamicin-Induced Nephrotoxicity by Modulating Distinctive Reactive Species |
| title_short | Curcumin and Vitamin C Attenuate Gentamicin-Induced Nephrotoxicity by Modulating Distinctive Reactive Species |
| title_sort | curcumin and vitamin c attenuate gentamicin induced nephrotoxicity by modulating distinctive reactive species |
| topic | gentamicin nephrotoxicity oxidative stress antioxidant lipid peroxidation malondialdehyde nitric oxide |
| url | https://www.mdpi.com/2218-1989/13/1/49 |
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