GhBEE3-Like gene regulated by brassinosteroids is involved in cotton drought tolerance
Brassinosteroids (BRs) are important phytohormones that play a vital role in plant drought tolerance, but their mechanisms in cotton (Gossypium hirsutum L.) are poorly understood. Numerous basic helix-loop-helix (bHLH) family genes are involved in the responses to both BRs and drought stress. GhBEE3...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1019146/full |
| _version_ | 1811342013763682304 |
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| author | Eryong Chen Eryong Chen Xiaobei Yang Xiaobei Yang Ruie Liu Mengke Zhang Mengke Zhang Meng Zhang Meng Zhang Feng Zhou Feng Zhou Dongxiao Li Dongxiao Li Haiyan Hu Haiyan Hu Chengwei Li Chengwei Li |
| author_facet | Eryong Chen Eryong Chen Xiaobei Yang Xiaobei Yang Ruie Liu Mengke Zhang Mengke Zhang Meng Zhang Meng Zhang Feng Zhou Feng Zhou Dongxiao Li Dongxiao Li Haiyan Hu Haiyan Hu Chengwei Li Chengwei Li |
| author_sort | Eryong Chen |
| collection | DOAJ |
| description | Brassinosteroids (BRs) are important phytohormones that play a vital role in plant drought tolerance, but their mechanisms in cotton (Gossypium hirsutum L.) are poorly understood. Numerous basic helix-loop-helix (bHLH) family genes are involved in the responses to both BRs and drought stress. GhBEE3-Like, a bHLH transcription factor, is repressed by both 24-epi-BL (an active BR substance) and PEG8000 (drought simulation) treatments in cotton. Moreover, GhBZR1, a crucial transcription factor in BR signaling pathway, directly binds to the E-box element in GhBEE3-Like promoter region and inhibits its expression, which has been confirmed by electrophoretic mobility shift assay (EMSA) and dual luciferase reporter assay. Functional analysis revealed that Arabidopsis with GhBEE3-Like overexpression had drought sensitive phenotype, while GhBEE3-Like knock-down cotton plants obtained by virus-induced gene silencing (VIGS) technology were more tolerant to drought stress. Furthermore, the expression levels of three stress-related genes, GhERD10, GhCDPK1 and GhRD26, were significantly higher in GhBEE3-Like knock-down cotton than in control cotton after drought treatment. These results suggest that GhBEE3-Like is inhibited by BRs which elevates the expressions of stress-related genes to enhance plant drought tolerance. This study lays the foundation for understanding the mechanisms of BR-regulated drought tolerance and establishment of drought-resistant cotton lines. |
| first_indexed | 2024-04-13T19:03:43Z |
| format | Article |
| id | doaj.art-9d40aaf357eb47699d312f1718e32818 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T19:03:43Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-9d40aaf357eb47699d312f1718e328182022-12-22T02:34:02ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10191461019146GhBEE3-Like gene regulated by brassinosteroids is involved in cotton drought toleranceEryong Chen0Eryong Chen1Xiaobei Yang2Xiaobei Yang3Ruie Liu4Mengke Zhang5Mengke Zhang6Meng Zhang7Meng Zhang8Feng Zhou9Feng Zhou10Dongxiao Li11Dongxiao Li12Haiyan Hu13Haiyan Hu14Chengwei Li15Chengwei Li16Henan Engineering Research Center of Crop Genome Editing, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaInternational Joint Laboratory of Plant Genetic Improvement and Soil Remediation, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaHenan Engineering Research Center of Crop Genome Editing, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaInternational Joint Laboratory of Plant Genetic Improvement and Soil Remediation, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaShanghai Center for Plant Stress Biology, National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, ChinaHenan Engineering Research Center of Crop Genome Editing, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaInternational Joint Laboratory of Plant Genetic Improvement and Soil Remediation, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaHenan Engineering Research Center of Crop Genome Editing, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaInternational Joint Laboratory of Plant Genetic Improvement and Soil Remediation, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaHenan Engineering Research Center of Crop Genome Editing, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaInternational Joint Laboratory of Plant Genetic Improvement and Soil Remediation, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaHenan Engineering Research Center of Crop Genome Editing, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaInternational Joint Laboratory of Plant Genetic Improvement and Soil Remediation, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaHenan Engineering Research Center of Crop Genome Editing, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaInternational Joint Laboratory of Plant Genetic Improvement and Soil Remediation, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaHenan Engineering Research Center of Crop Genome Editing, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, ChinaCollege of Biological Engineering, Henan University of Technology, Zhengzhou, ChinaBrassinosteroids (BRs) are important phytohormones that play a vital role in plant drought tolerance, but their mechanisms in cotton (Gossypium hirsutum L.) are poorly understood. Numerous basic helix-loop-helix (bHLH) family genes are involved in the responses to both BRs and drought stress. GhBEE3-Like, a bHLH transcription factor, is repressed by both 24-epi-BL (an active BR substance) and PEG8000 (drought simulation) treatments in cotton. Moreover, GhBZR1, a crucial transcription factor in BR signaling pathway, directly binds to the E-box element in GhBEE3-Like promoter region and inhibits its expression, which has been confirmed by electrophoretic mobility shift assay (EMSA) and dual luciferase reporter assay. Functional analysis revealed that Arabidopsis with GhBEE3-Like overexpression had drought sensitive phenotype, while GhBEE3-Like knock-down cotton plants obtained by virus-induced gene silencing (VIGS) technology were more tolerant to drought stress. Furthermore, the expression levels of three stress-related genes, GhERD10, GhCDPK1 and GhRD26, were significantly higher in GhBEE3-Like knock-down cotton than in control cotton after drought treatment. These results suggest that GhBEE3-Like is inhibited by BRs which elevates the expressions of stress-related genes to enhance plant drought tolerance. This study lays the foundation for understanding the mechanisms of BR-regulated drought tolerance and establishment of drought-resistant cotton lines.https://www.frontiersin.org/articles/10.3389/fpls.2022.1019146/fullGhBEE3-LikebHLH transcription factorbrassinosteroids (BRs)GhBZR1drought tolerancecotton (Gossypium hirsutum L.) |
| spellingShingle | Eryong Chen Eryong Chen Xiaobei Yang Xiaobei Yang Ruie Liu Mengke Zhang Mengke Zhang Meng Zhang Meng Zhang Feng Zhou Feng Zhou Dongxiao Li Dongxiao Li Haiyan Hu Haiyan Hu Chengwei Li Chengwei Li GhBEE3-Like gene regulated by brassinosteroids is involved in cotton drought tolerance Frontiers in Plant Science GhBEE3-Like bHLH transcription factor brassinosteroids (BRs) GhBZR1 drought tolerance cotton (Gossypium hirsutum L.) |
| title | GhBEE3-Like gene regulated by brassinosteroids is involved in cotton drought tolerance |
| title_full | GhBEE3-Like gene regulated by brassinosteroids is involved in cotton drought tolerance |
| title_fullStr | GhBEE3-Like gene regulated by brassinosteroids is involved in cotton drought tolerance |
| title_full_unstemmed | GhBEE3-Like gene regulated by brassinosteroids is involved in cotton drought tolerance |
| title_short | GhBEE3-Like gene regulated by brassinosteroids is involved in cotton drought tolerance |
| title_sort | ghbee3 like gene regulated by brassinosteroids is involved in cotton drought tolerance |
| topic | GhBEE3-Like bHLH transcription factor brassinosteroids (BRs) GhBZR1 drought tolerance cotton (Gossypium hirsutum L.) |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1019146/full |
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