OsDXR interacts with OsMORF1 to regulate chloroplast development and the RNA editing of chloroplast genes in rice
Plant chlorophyll biosynthesis and chloroplast development are two complex processes that are regulated by exogenous and endogenous factors. In this study, we identified OsDXR, a gene encoding a reductoisomerase that positively regulates chlorophyll biosynthesis and chloroplast development in rice....
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311922000831 |
| _version_ | 1827989352797437952 |
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| author | Peng-hui CAO Di WANG Su GAO Xi LIU Zhong-ying QIAO Yu-lin XIE Ming-hui DONG Tan-xiao DU Xian ZHANG Rui ZHANG Jian-hui JI |
| author_facet | Peng-hui CAO Di WANG Su GAO Xi LIU Zhong-ying QIAO Yu-lin XIE Ming-hui DONG Tan-xiao DU Xian ZHANG Rui ZHANG Jian-hui JI |
| author_sort | Peng-hui CAO |
| collection | DOAJ |
| description | Plant chlorophyll biosynthesis and chloroplast development are two complex processes that are regulated by exogenous and endogenous factors. In this study, we identified OsDXR, a gene encoding a reductoisomerase that positively regulates chlorophyll biosynthesis and chloroplast development in rice. OsDXR knock-out lines displayed the albino phenotype and could not complete the whole life cycle process. OsDXR was highly expressed in rice leaves, and subcellular localization indicated that OsDXR is a chloroplast protein. Many genes involved in chlorophyll biosynthesis and chloroplast development were differentially expressed in the OsDXR knock-out lines compared to the wild type. Moreover, we found that the RNA editing efficiencies of ndhA-1019 and rpl2-1 were significantly reduced in the OsDXR knock-out lines. Furthermore, OsDXR interacted with the RNA editing factor OsMORF1 in a yeast two-hybrid screen and bimolecular fluorescence complementation assay. Finally, disruption of the plastidial 2-C-methyl-derythritol-4-phosphate pathway resulted in defects in chloroplast development and the RNA editing of chloroplast genes. |
| first_indexed | 2024-04-10T00:18:04Z |
| format | Article |
| id | doaj.art-9d44d054c80f40dc8571ccb49e74c4a2 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-04-10T00:18:04Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-9d44d054c80f40dc8571ccb49e74c4a22023-03-16T05:03:40ZengElsevierJournal of Integrative Agriculture2095-31192023-03-01223669678OsDXR interacts with OsMORF1 to regulate chloroplast development and the RNA editing of chloroplast genes in ricePeng-hui CAO0Di WANG1Su GAO2Xi LIU3Zhong-ying QIAO4Yu-lin XIE5Ming-hui DONG6Tan-xiao DU7Xian ZHANG8Rui ZHANG9Jian-hui JI10Institute of Agricultural Sciences in Taihu Area of Jiangsu, Suzhou 215155, P.R.ChinaHuaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Huai'an 223001, P.R.ChinaSchool of Life Sciences, Huaiyin Normal University, Huai'an 223300, P.R.ChinaSchool of Life Sciences, Huaiyin Normal University, Huai'an 223300, P.R.China; Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake, Regional Cooperative Innovation Center for Modern Agriculture and Environmental Protection, Huaiyin Normal University, Huai'an 223300, P.R.China; Correspondence LIU XiInstitute of Agricultural Sciences in Taihu Area of Jiangsu, Suzhou 215155, P.R.ChinaInstitute of Agricultural Sciences in Taihu Area of Jiangsu, Suzhou 215155, P.R.ChinaInstitute of Agricultural Sciences in Taihu Area of Jiangsu, Suzhou 215155, P.R.ChinaSchool of Life Sciences, Huaiyin Normal University, Huai'an 223300, P.R.ChinaSchool of Life Sciences, Huaiyin Normal University, Huai'an 223300, P.R.ChinaSchool of Life Sciences, Huaiyin Normal University, Huai'an 223300, P.R.ChinaSchool of Life Sciences, Huaiyin Normal University, Huai'an 223300, P.R.China; Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake, Regional Cooperative Innovation Center for Modern Agriculture and Environmental Protection, Huaiyin Normal University, Huai'an 223300, P.R.China; Correspondence JI Jian-huiPlant chlorophyll biosynthesis and chloroplast development are two complex processes that are regulated by exogenous and endogenous factors. In this study, we identified OsDXR, a gene encoding a reductoisomerase that positively regulates chlorophyll biosynthesis and chloroplast development in rice. OsDXR knock-out lines displayed the albino phenotype and could not complete the whole life cycle process. OsDXR was highly expressed in rice leaves, and subcellular localization indicated that OsDXR is a chloroplast protein. Many genes involved in chlorophyll biosynthesis and chloroplast development were differentially expressed in the OsDXR knock-out lines compared to the wild type. Moreover, we found that the RNA editing efficiencies of ndhA-1019 and rpl2-1 were significantly reduced in the OsDXR knock-out lines. Furthermore, OsDXR interacted with the RNA editing factor OsMORF1 in a yeast two-hybrid screen and bimolecular fluorescence complementation assay. Finally, disruption of the plastidial 2-C-methyl-derythritol-4-phosphate pathway resulted in defects in chloroplast development and the RNA editing of chloroplast genes.http://www.sciencedirect.com/science/article/pii/S2095311922000831riceOsDXRChloroplast developmentRNA editingOsMORF1 |
| spellingShingle | Peng-hui CAO Di WANG Su GAO Xi LIU Zhong-ying QIAO Yu-lin XIE Ming-hui DONG Tan-xiao DU Xian ZHANG Rui ZHANG Jian-hui JI OsDXR interacts with OsMORF1 to regulate chloroplast development and the RNA editing of chloroplast genes in rice Journal of Integrative Agriculture rice OsDXR Chloroplast development RNA editing OsMORF1 |
| title | OsDXR interacts with OsMORF1 to regulate chloroplast development and the RNA editing of chloroplast genes in rice |
| title_full | OsDXR interacts with OsMORF1 to regulate chloroplast development and the RNA editing of chloroplast genes in rice |
| title_fullStr | OsDXR interacts with OsMORF1 to regulate chloroplast development and the RNA editing of chloroplast genes in rice |
| title_full_unstemmed | OsDXR interacts with OsMORF1 to regulate chloroplast development and the RNA editing of chloroplast genes in rice |
| title_short | OsDXR interacts with OsMORF1 to regulate chloroplast development and the RNA editing of chloroplast genes in rice |
| title_sort | osdxr interacts with osmorf1 to regulate chloroplast development and the rna editing of chloroplast genes in rice |
| topic | rice OsDXR Chloroplast development RNA editing OsMORF1 |
| url | http://www.sciencedirect.com/science/article/pii/S2095311922000831 |
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