INTERCEPT Pathogen Reduction in Platelet Concentrates, in Contrast to Gamma Irradiation, Induces the Formation of <i>trans</i>-Arachidonic Acids and Affects Eicosanoid Release during Storage
Pathogen inactivation techniques for blood products have been implemented to optimize clinically safe blood components supply. The INTERCEPT system uses amotosalen together with ultraviolet light wavelength A (UVA) irradiation. Irradiation-induced inactivation of nucleic acids may actually be accomp...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/12/9/1258 |
| _version_ | 1797490687529189376 |
|---|---|
| author | Gerda C. Leitner Gerhard Hagn Laura Niederstaetter Andrea Bileck Kerstin Plessl-Walder Michaela Horvath Vera Kolovratova Andreas Tanzmann Alexander Tolios Werner Rabitsch Philipp Wohlfarth Christopher Gerner |
| author_facet | Gerda C. Leitner Gerhard Hagn Laura Niederstaetter Andrea Bileck Kerstin Plessl-Walder Michaela Horvath Vera Kolovratova Andreas Tanzmann Alexander Tolios Werner Rabitsch Philipp Wohlfarth Christopher Gerner |
| author_sort | Gerda C. Leitner |
| collection | DOAJ |
| description | Pathogen inactivation techniques for blood products have been implemented to optimize clinically safe blood components supply. The INTERCEPT system uses amotosalen together with ultraviolet light wavelength A (UVA) irradiation. Irradiation-induced inactivation of nucleic acids may actually be accompanied by modifications of chemically reactive polyunsaturated fatty acids known to be important mediators of platelet functions. Thus, here, we investigated eicosanoids and the related fatty acids released upon treatment and during storage of platelet concentrates for 7 days, complemented by the analysis of functional and metabolic consequences of these treatments. Metabolic and functional issues like glucose consumption, lactate formation, platelet aggregation, and clot firmness hardly differed between the two treatment groups. In contrast to gamma irradiation, here, we demonstrated that INTERCEPT treatment immediately caused new formation of <i>trans</i>-arachidonic acid isoforms, while 11-hydroxyeicosatetraenoic acid (11-HETE) and 15-HETE were increased and two hydroperoxyoctadecadienoic acid (HpODE) isoforms decreased. During further storage, these alterations remained stable, while the release of 12-lipoxygenase (12-LOX) products such as 12-HETE and 12-hydroxyeicosapentaenoic acid (12-HEPE) was further attenuated. In vitro synthesis of <i>trans</i>-arachidonic acid isoforms suggested that thiol radicals formed by UVA treatment may be responsible for the INTERCEPT-specific effects observed in platelet concentrates. It is reasonable to assume that UVA-induced molecules may have specific biological effects which need to be further investigated. |
| first_indexed | 2024-03-10T00:36:36Z |
| format | Article |
| id | doaj.art-9d4793fa7ebc48e2af399071db12c41f |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T00:36:36Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-9d4793fa7ebc48e2af399071db12c41f2023-11-23T15:15:37ZengMDPI AGBiomolecules2218-273X2022-09-01129125810.3390/biom12091258INTERCEPT Pathogen Reduction in Platelet Concentrates, in Contrast to Gamma Irradiation, Induces the Formation of <i>trans</i>-Arachidonic Acids and Affects Eicosanoid Release during StorageGerda C. Leitner0Gerhard Hagn1Laura Niederstaetter2Andrea Bileck3Kerstin Plessl-Walder4Michaela Horvath5Vera Kolovratova6Andreas Tanzmann7Alexander Tolios8Werner Rabitsch9Philipp Wohlfarth10Christopher Gerner11Department of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Analytical Chemistry, Faculty of Chemistry, University of Vienna, 1090 Vienna, AustriaDepartment of Analytical Chemistry, Faculty of Chemistry, University of Vienna, 1090 Vienna, AustriaDepartment of Analytical Chemistry, Faculty of Chemistry, University of Vienna, 1090 Vienna, AustriaDivision of Biomedical Science, University of Applied Sciences, FH Campus Wien, 1100 Vienna, AustriaDepartment of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, 1090 Vienna, AustriaInternal Medicine 1, Stem Cell Transplantation Unit, Medical University of Vienna, 1090 Vienna, AustriaInternal Medicine 1, Stem Cell Transplantation Unit, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Analytical Chemistry, Faculty of Chemistry, University of Vienna, 1090 Vienna, AustriaPathogen inactivation techniques for blood products have been implemented to optimize clinically safe blood components supply. The INTERCEPT system uses amotosalen together with ultraviolet light wavelength A (UVA) irradiation. Irradiation-induced inactivation of nucleic acids may actually be accompanied by modifications of chemically reactive polyunsaturated fatty acids known to be important mediators of platelet functions. Thus, here, we investigated eicosanoids and the related fatty acids released upon treatment and during storage of platelet concentrates for 7 days, complemented by the analysis of functional and metabolic consequences of these treatments. Metabolic and functional issues like glucose consumption, lactate formation, platelet aggregation, and clot firmness hardly differed between the two treatment groups. In contrast to gamma irradiation, here, we demonstrated that INTERCEPT treatment immediately caused new formation of <i>trans</i>-arachidonic acid isoforms, while 11-hydroxyeicosatetraenoic acid (11-HETE) and 15-HETE were increased and two hydroperoxyoctadecadienoic acid (HpODE) isoforms decreased. During further storage, these alterations remained stable, while the release of 12-lipoxygenase (12-LOX) products such as 12-HETE and 12-hydroxyeicosapentaenoic acid (12-HEPE) was further attenuated. In vitro synthesis of <i>trans</i>-arachidonic acid isoforms suggested that thiol radicals formed by UVA treatment may be responsible for the INTERCEPT-specific effects observed in platelet concentrates. It is reasonable to assume that UVA-induced molecules may have specific biological effects which need to be further investigated.https://www.mdpi.com/2218-273X/12/9/1258eicosanoidshigh-resolution mass spectrometryliquid chromatographypathogen reductionplatelet concentratesplatelets |
| spellingShingle | Gerda C. Leitner Gerhard Hagn Laura Niederstaetter Andrea Bileck Kerstin Plessl-Walder Michaela Horvath Vera Kolovratova Andreas Tanzmann Alexander Tolios Werner Rabitsch Philipp Wohlfarth Christopher Gerner INTERCEPT Pathogen Reduction in Platelet Concentrates, in Contrast to Gamma Irradiation, Induces the Formation of <i>trans</i>-Arachidonic Acids and Affects Eicosanoid Release during Storage Biomolecules eicosanoids high-resolution mass spectrometry liquid chromatography pathogen reduction platelet concentrates platelets |
| title | INTERCEPT Pathogen Reduction in Platelet Concentrates, in Contrast to Gamma Irradiation, Induces the Formation of <i>trans</i>-Arachidonic Acids and Affects Eicosanoid Release during Storage |
| title_full | INTERCEPT Pathogen Reduction in Platelet Concentrates, in Contrast to Gamma Irradiation, Induces the Formation of <i>trans</i>-Arachidonic Acids and Affects Eicosanoid Release during Storage |
| title_fullStr | INTERCEPT Pathogen Reduction in Platelet Concentrates, in Contrast to Gamma Irradiation, Induces the Formation of <i>trans</i>-Arachidonic Acids and Affects Eicosanoid Release during Storage |
| title_full_unstemmed | INTERCEPT Pathogen Reduction in Platelet Concentrates, in Contrast to Gamma Irradiation, Induces the Formation of <i>trans</i>-Arachidonic Acids and Affects Eicosanoid Release during Storage |
| title_short | INTERCEPT Pathogen Reduction in Platelet Concentrates, in Contrast to Gamma Irradiation, Induces the Formation of <i>trans</i>-Arachidonic Acids and Affects Eicosanoid Release during Storage |
| title_sort | intercept pathogen reduction in platelet concentrates in contrast to gamma irradiation induces the formation of i trans i arachidonic acids and affects eicosanoid release during storage |
| topic | eicosanoids high-resolution mass spectrometry liquid chromatography pathogen reduction platelet concentrates platelets |
| url | https://www.mdpi.com/2218-273X/12/9/1258 |
| work_keys_str_mv | AT gerdacleitner interceptpathogenreductioninplateletconcentratesincontrasttogammairradiationinducestheformationofitransiarachidonicacidsandaffectseicosanoidreleaseduringstorage AT gerhardhagn interceptpathogenreductioninplateletconcentratesincontrasttogammairradiationinducestheformationofitransiarachidonicacidsandaffectseicosanoidreleaseduringstorage AT lauraniederstaetter interceptpathogenreductioninplateletconcentratesincontrasttogammairradiationinducestheformationofitransiarachidonicacidsandaffectseicosanoidreleaseduringstorage AT andreabileck interceptpathogenreductioninplateletconcentratesincontrasttogammairradiationinducestheformationofitransiarachidonicacidsandaffectseicosanoidreleaseduringstorage AT kerstinplesslwalder interceptpathogenreductioninplateletconcentratesincontrasttogammairradiationinducestheformationofitransiarachidonicacidsandaffectseicosanoidreleaseduringstorage AT michaelahorvath interceptpathogenreductioninplateletconcentratesincontrasttogammairradiationinducestheformationofitransiarachidonicacidsandaffectseicosanoidreleaseduringstorage AT verakolovratova interceptpathogenreductioninplateletconcentratesincontrasttogammairradiationinducestheformationofitransiarachidonicacidsandaffectseicosanoidreleaseduringstorage AT andreastanzmann interceptpathogenreductioninplateletconcentratesincontrasttogammairradiationinducestheformationofitransiarachidonicacidsandaffectseicosanoidreleaseduringstorage AT alexandertolios interceptpathogenreductioninplateletconcentratesincontrasttogammairradiationinducestheformationofitransiarachidonicacidsandaffectseicosanoidreleaseduringstorage AT wernerrabitsch interceptpathogenreductioninplateletconcentratesincontrasttogammairradiationinducestheformationofitransiarachidonicacidsandaffectseicosanoidreleaseduringstorage AT philippwohlfarth interceptpathogenreductioninplateletconcentratesincontrasttogammairradiationinducestheformationofitransiarachidonicacidsandaffectseicosanoidreleaseduringstorage AT christophergerner interceptpathogenreductioninplateletconcentratesincontrasttogammairradiationinducestheformationofitransiarachidonicacidsandaffectseicosanoidreleaseduringstorage |