The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
Abstract The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium melilo...
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| Format: | Article |
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Nature Portfolio
2017-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-00730-7 |
| _version_ | 1818845721622740992 |
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| author | Nicolás Toro Pablo J. Villadas María Dolores Molina-Sánchez Pilar Navarro-Gómez José M. Vinardell Lidia Cuesta-Berrio Miguel A. Rodríguez-Carvajal |
| author_facet | Nicolás Toro Pablo J. Villadas María Dolores Molina-Sánchez Pilar Navarro-Gómez José M. Vinardell Lidia Cuesta-Berrio Miguel A. Rodríguez-Carvajal |
| author_sort | Nicolás Toro |
| collection | DOAJ |
| description | Abstract The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium meliloti population. Whole-genome sequencing revealed that a large DNA region of the symbiotic plasmid pSymB was replaced in some isolates with a similar synteny block carrying densely clustered SNPs and displaying gene acquisition and loss. Two different versions of this genomic island of differentiation (GID) generated by multiple genetic exchanges over time appear to have arisen recently, through recombination in a particular clade within this population. In addition, these isolates display resistance to phages from the same geographic region, probably due to the modification of surface components by the acquired genes. Our results suggest that an underlying process of early ecological and genetic differentiation in S. meliloti is primarily triggered by acquisition of genes that confer resistance to soil phages within particular large genomic DNA regions prone to recombination. |
| first_indexed | 2024-12-19T05:34:10Z |
| format | Article |
| id | doaj.art-9d49c55c01cd487faa81b8622b75699d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T05:34:10Z |
| publishDate | 2017-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9d49c55c01cd487faa81b8622b75699d2022-12-21T20:34:10ZengNature PortfolioScientific Reports2045-23222017-04-017111210.1038/s41598-017-00730-7The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti populationNicolás Toro0Pablo J. Villadas1María Dolores Molina-Sánchez2Pilar Navarro-Gómez3José M. Vinardell4Lidia Cuesta-Berrio5Miguel A. Rodríguez-Carvajal6Structure, Dynamics and Function of Rhizobacterial Genomes, Grupo de Ecología Genética de la Rizosfera, Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, Consejo Superior de Investigaciones CientíficasStructure, Dynamics and Function of Rhizobacterial Genomes, Grupo de Ecología Genética de la Rizosfera, Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, Consejo Superior de Investigaciones CientíficasStructure, Dynamics and Function of Rhizobacterial Genomes, Grupo de Ecología Genética de la Rizosfera, Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, Consejo Superior de Investigaciones CientíficasDepartamento de Microbiología, Facultad de Biología, Universidad de SevillaDepartamento de Microbiología, Facultad de Biología, Universidad de SevillaDepartamento de Química Orgánica, Universidad de SevillaDepartamento de Química Orgánica, Universidad de SevillaAbstract The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium meliloti population. Whole-genome sequencing revealed that a large DNA region of the symbiotic plasmid pSymB was replaced in some isolates with a similar synteny block carrying densely clustered SNPs and displaying gene acquisition and loss. Two different versions of this genomic island of differentiation (GID) generated by multiple genetic exchanges over time appear to have arisen recently, through recombination in a particular clade within this population. In addition, these isolates display resistance to phages from the same geographic region, probably due to the modification of surface components by the acquired genes. Our results suggest that an underlying process of early ecological and genetic differentiation in S. meliloti is primarily triggered by acquisition of genes that confer resistance to soil phages within particular large genomic DNA regions prone to recombination.https://doi.org/10.1038/s41598-017-00730-7 |
| spellingShingle | Nicolás Toro Pablo J. Villadas María Dolores Molina-Sánchez Pilar Navarro-Gómez José M. Vinardell Lidia Cuesta-Berrio Miguel A. Rodríguez-Carvajal The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population Scientific Reports |
| title | The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population |
| title_full | The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population |
| title_fullStr | The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population |
| title_full_unstemmed | The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population |
| title_short | The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population |
| title_sort | underlying process of early ecological and genetic differentiation in a facultative mutualistic sinorhizobium meliloti population |
| url | https://doi.org/10.1038/s41598-017-00730-7 |
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