A Portable Device for the Generation of Drug-Loaded Three-Compartmental Fibers Containing Metronidazole and Iodine for Topical Application
The use of combination therapies for the treatment of a range of conditions is now well established, with the component drugs usually being delivered either as distinct medicaments or combination products that contain physical mixes of the two active ingredients. There is, however, a compelling argu...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/12/4/373 |
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| author | Francis Brako Chaojie Luo Rupy Kaur Matharu Lena Ciric Anthony Harker Mohan Edirisinghe Duncan Q. M. Craig |
| author_facet | Francis Brako Chaojie Luo Rupy Kaur Matharu Lena Ciric Anthony Harker Mohan Edirisinghe Duncan Q. M. Craig |
| author_sort | Francis Brako |
| collection | DOAJ |
| description | The use of combination therapies for the treatment of a range of conditions is now well established, with the component drugs usually being delivered either as distinct medicaments or combination products that contain physical mixes of the two active ingredients. There is, however, a compelling argument for the development of compartmentalised systems whereby the release, stability and incorporation environment of the different drugs may be tailored. Here we outline the development of polymeric fine fiber systems whereby two drugs used for the treatment of wounds may be separately incorporated. Fibers were delivered using a newly developed handheld electrospinning device that allows treatment at the site of need. Crucially, the delivery system is portable and may be used for the administration of drug-loaded fibers directly into the wound in situ, thereby potentially allowing domiciliary or site-of-trauma administration. The three-layered fiber developed in this study has polyethylene glycol as the outermost layer, serving as a structural support for the inner layers. The inner layers comprised iodine complexed with polyvinylpyrrolidone (PVP) and metronidazole dispersed in polycaprolactone (PCL) as a slow release core. The systems were characterized in terms of structure and architecture using scanning electron microscopy, transmission electron microscopy, attenuated total reflection Fourier transform infrared spectroscopy and diffractometry. As antibacterial creams are still used for managing infected wounds, the performance of our trilayered fiber was studied in comparison with creams containing similar active drugs. Drug release was measured by UV analysis, while antimicrobial efficiency was measured using agar diffusion and suspension methods. It was found that the trilayered systems, averaging 3.16 µm in diameter, released more drug over the study period and were confirmed by the microbacterial studies to be more effective against <i>P. aeruginosa</i>, a bacterium commonly implicated in infected wounds. Overall, the portable system has been shown to be capable of not only incorporating the two drugs in distinct layers but also of delivering adequate amounts of drugs for a more effective antibacterial activity. The portability of the device and its ability to generate distinct layers of multiple active ingredients make it promising for further development for wound healing applications in terms of both practical applicability and antimicrobial efficacy. |
| first_indexed | 2024-03-10T20:23:25Z |
| format | Article |
| id | doaj.art-9d51c85d83774335b9431af2947b62e0 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T20:23:25Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-9d51c85d83774335b9431af2947b62e02023-11-19T22:01:26ZengMDPI AGPharmaceutics1999-49232020-04-0112437310.3390/pharmaceutics12040373A Portable Device for the Generation of Drug-Loaded Three-Compartmental Fibers Containing Metronidazole and Iodine for Topical ApplicationFrancis Brako0Chaojie Luo1Rupy Kaur Matharu2Lena Ciric3Anthony Harker4Mohan Edirisinghe5Duncan Q. M. Craig6Medway School of Pharmacy, Universities of Kent and Greenwich, Chatham ME4 4TB, UKDepartment of Mechanical Engineering, University College London, Torrington Place, London WC1E 7JE, UKDepartment of Mechanical Engineering, University College London, Torrington Place, London WC1E 7JE, UKDepartment of Civil, Environmental & Geomatic Engineering, University College London, Chadwick Building, Gower Street, London WC1E 6BT, UKDepartment of Physics and Astronomy and London Centre for Nanotechnology, University College London, London WC1E 6BT, UKDepartment of Mechanical Engineering, University College London, Torrington Place, London WC1E 7JE, UKSchool of Pharmacy, University College London, 29–39 Brunswick Square, London WC1N 1AX, UKThe use of combination therapies for the treatment of a range of conditions is now well established, with the component drugs usually being delivered either as distinct medicaments or combination products that contain physical mixes of the two active ingredients. There is, however, a compelling argument for the development of compartmentalised systems whereby the release, stability and incorporation environment of the different drugs may be tailored. Here we outline the development of polymeric fine fiber systems whereby two drugs used for the treatment of wounds may be separately incorporated. Fibers were delivered using a newly developed handheld electrospinning device that allows treatment at the site of need. Crucially, the delivery system is portable and may be used for the administration of drug-loaded fibers directly into the wound in situ, thereby potentially allowing domiciliary or site-of-trauma administration. The three-layered fiber developed in this study has polyethylene glycol as the outermost layer, serving as a structural support for the inner layers. The inner layers comprised iodine complexed with polyvinylpyrrolidone (PVP) and metronidazole dispersed in polycaprolactone (PCL) as a slow release core. The systems were characterized in terms of structure and architecture using scanning electron microscopy, transmission electron microscopy, attenuated total reflection Fourier transform infrared spectroscopy and diffractometry. As antibacterial creams are still used for managing infected wounds, the performance of our trilayered fiber was studied in comparison with creams containing similar active drugs. Drug release was measured by UV analysis, while antimicrobial efficiency was measured using agar diffusion and suspension methods. It was found that the trilayered systems, averaging 3.16 µm in diameter, released more drug over the study period and were confirmed by the microbacterial studies to be more effective against <i>P. aeruginosa</i>, a bacterium commonly implicated in infected wounds. Overall, the portable system has been shown to be capable of not only incorporating the two drugs in distinct layers but also of delivering adequate amounts of drugs for a more effective antibacterial activity. The portability of the device and its ability to generate distinct layers of multiple active ingredients make it promising for further development for wound healing applications in terms of both practical applicability and antimicrobial efficacy.https://www.mdpi.com/1999-4923/12/4/373trilayered fibersportable electrospinningwound dressingcombination therapycompartmental drug delivery |
| spellingShingle | Francis Brako Chaojie Luo Rupy Kaur Matharu Lena Ciric Anthony Harker Mohan Edirisinghe Duncan Q. M. Craig A Portable Device for the Generation of Drug-Loaded Three-Compartmental Fibers Containing Metronidazole and Iodine for Topical Application Pharmaceutics trilayered fibers portable electrospinning wound dressing combination therapy compartmental drug delivery |
| title | A Portable Device for the Generation of Drug-Loaded Three-Compartmental Fibers Containing Metronidazole and Iodine for Topical Application |
| title_full | A Portable Device for the Generation of Drug-Loaded Three-Compartmental Fibers Containing Metronidazole and Iodine for Topical Application |
| title_fullStr | A Portable Device for the Generation of Drug-Loaded Three-Compartmental Fibers Containing Metronidazole and Iodine for Topical Application |
| title_full_unstemmed | A Portable Device for the Generation of Drug-Loaded Three-Compartmental Fibers Containing Metronidazole and Iodine for Topical Application |
| title_short | A Portable Device for the Generation of Drug-Loaded Three-Compartmental Fibers Containing Metronidazole and Iodine for Topical Application |
| title_sort | portable device for the generation of drug loaded three compartmental fibers containing metronidazole and iodine for topical application |
| topic | trilayered fibers portable electrospinning wound dressing combination therapy compartmental drug delivery |
| url | https://www.mdpi.com/1999-4923/12/4/373 |
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