Virulence gene， molecular typing and drug resistance of 210 Vibrio Parahaemolyticus strains in Fengxian District， Shanghai
ObjectiveTo determine the drug resistance， main virulence genes and molecular typing of Vibrio parahaemolyticus from multiple sources in Fengxian District， Shanghai.MethodsA total of 210 strains of Vibrio parahaemolyticus were isolated in Fengxian District， Shanghai， from 2018 to 2020. Then drug sus...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
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Shanghai Preventive Medicine Association
2022-09-01
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| Series: | Shanghai yufang yixue |
| Subjects: | |
| Online Access: | http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2022.21765 |
| _version_ | 1797987002504708096 |
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| author | ZHAO Hongwei XIE Xiaohong TAO Lixin SHEN Chunli WANG Jie SHEN Li |
| author_facet | ZHAO Hongwei XIE Xiaohong TAO Lixin SHEN Chunli WANG Jie SHEN Li |
| author_sort | ZHAO Hongwei |
| collection | DOAJ |
| description | ObjectiveTo determine the drug resistance， main virulence genes and molecular typing of Vibrio parahaemolyticus from multiple sources in Fengxian District， Shanghai.MethodsA total of 210 strains of Vibrio parahaemolyticus were isolated in Fengxian District， Shanghai， from 2018 to 2020. Then drug susceptibility tests of 17 antibiotics， virulence gene detection of thermolabilehemolysin （tlh）， thermostable direct hemolysin （tdh） and thermostable direct hemolysin-relatedhemolysin （trh）， and pulsed-field gel electrophoresis （PFGE） were conducted.ResultsA total of 147 drug-resistant strains were detected， with the drug resistance of 70.00%. Among them， 126 strains were resistant to single antibiotic， mainly resistant to CFZ； 15 strains were resistant to two antibiotics， mainly resistant to CFZ and AMP； and 6 strains of MDR， mainly resistant to AMP， SXT， CFZ， CTX， CAZ and FEP. The AMP resistance of food-borne strains was higher than that of human-derived strains （P<0.05）. All 210 strains of Vibrio parahaemolyticus carried the virulence gene tlh. Furthermore， 84 human-derived strains carried tdh （89.29%） and trh （7.14%）. In contrast， 126 food-borne strains carried trh （1.59%）， while did not carry tdh. In additionally， 173 band patterns were determined by PFGE， sharing the similarity of 48.80%‒100.00%， among which major clones were prevalent.ConclusionVibrio parahaemolyticus in Fengxian District has multi-drug resistance， especially the resistance to β-lactam antibiotics. The prevalence of virulence gene remains high in human-derived strains. Furthermore， major clones are prevalent in human-derived strains， though the PFGE banding patterns are generally diverse. Due to high risk of food-borne diseases， this study warrants strengthening the prevention and control against Vibrio parahaemolyticus. |
| first_indexed | 2024-04-11T07:42:00Z |
| format | Article |
| id | doaj.art-9d53796d6f1f45e484517e253584f615 |
| institution | Directory Open Access Journal |
| issn | 1004-9231 |
| language | zho |
| last_indexed | 2024-04-11T07:42:00Z |
| publishDate | 2022-09-01 |
| publisher | Shanghai Preventive Medicine Association |
| record_format | Article |
| series | Shanghai yufang yixue |
| spelling | doaj.art-9d53796d6f1f45e484517e253584f6152022-12-22T04:36:30ZzhoShanghai Preventive Medicine AssociationShanghai yufang yixue1004-92312022-09-0134990190510.19428/j.cnki.sjpm.2022.217651004-9231（2022）09-0901-05Virulence gene， molecular typing and drug resistance of 210 Vibrio Parahaemolyticus strains in Fengxian District， ShanghaiZHAO HongweiXIE XiaohongTAO LixinSHEN ChunliWANG JieSHEN LiObjectiveTo determine the drug resistance， main virulence genes and molecular typing of Vibrio parahaemolyticus from multiple sources in Fengxian District， Shanghai.MethodsA total of 210 strains of Vibrio parahaemolyticus were isolated in Fengxian District， Shanghai， from 2018 to 2020. Then drug susceptibility tests of 17 antibiotics， virulence gene detection of thermolabilehemolysin （tlh）， thermostable direct hemolysin （tdh） and thermostable direct hemolysin-relatedhemolysin （trh）， and pulsed-field gel electrophoresis （PFGE） were conducted.ResultsA total of 147 drug-resistant strains were detected， with the drug resistance of 70.00%. Among them， 126 strains were resistant to single antibiotic， mainly resistant to CFZ； 15 strains were resistant to two antibiotics， mainly resistant to CFZ and AMP； and 6 strains of MDR， mainly resistant to AMP， SXT， CFZ， CTX， CAZ and FEP. The AMP resistance of food-borne strains was higher than that of human-derived strains （P<0.05）. All 210 strains of Vibrio parahaemolyticus carried the virulence gene tlh. Furthermore， 84 human-derived strains carried tdh （89.29%） and trh （7.14%）. In contrast， 126 food-borne strains carried trh （1.59%）， while did not carry tdh. In additionally， 173 band patterns were determined by PFGE， sharing the similarity of 48.80%‒100.00%， among which major clones were prevalent.ConclusionVibrio parahaemolyticus in Fengxian District has multi-drug resistance， especially the resistance to β-lactam antibiotics. The prevalence of virulence gene remains high in human-derived strains. Furthermore， major clones are prevalent in human-derived strains， though the PFGE banding patterns are generally diverse. Due to high risk of food-borne diseases， this study warrants strengthening the prevention and control against Vibrio parahaemolyticus.http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2022.21765vibrio parahaemolyticusvirulence genepulsed field gel electrophoresisdrug resistance |
| spellingShingle | ZHAO Hongwei XIE Xiaohong TAO Lixin SHEN Chunli WANG Jie SHEN Li Virulence gene， molecular typing and drug resistance of 210 Vibrio Parahaemolyticus strains in Fengxian District， Shanghai Shanghai yufang yixue vibrio parahaemolyticus virulence gene pulsed field gel electrophoresis drug resistance |
| title | Virulence gene， molecular typing and drug resistance of 210 Vibrio Parahaemolyticus strains in Fengxian District， Shanghai |
| title_full | Virulence gene， molecular typing and drug resistance of 210 Vibrio Parahaemolyticus strains in Fengxian District， Shanghai |
| title_fullStr | Virulence gene， molecular typing and drug resistance of 210 Vibrio Parahaemolyticus strains in Fengxian District， Shanghai |
| title_full_unstemmed | Virulence gene， molecular typing and drug resistance of 210 Vibrio Parahaemolyticus strains in Fengxian District， Shanghai |
| title_short | Virulence gene， molecular typing and drug resistance of 210 Vibrio Parahaemolyticus strains in Fengxian District， Shanghai |
| title_sort | virulence gene molecular typing and drug resistance of 210 vibrio parahaemolyticus strains in fengxian district shanghai |
| topic | vibrio parahaemolyticus virulence gene pulsed field gel electrophoresis drug resistance |
| url | http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2022.21765 |
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