Anther culture and highly frequent and rapid establishment of embryogenic cell suspensions from Hordeum vulgare L(大麦花药培养及其胚性悬浮细胞系的高频快速建立)

Anther culture and highly frequent and rapid establishment of embryogenic cell suspensions from Hordeum vulgare L(大麦花药培养及其胚性悬浮细胞系的高频快速建立)

研究了(1)在低温预处理过程中,不同相对湿度对大麦花药出愈率的影响;(2)低温处理愈伤组织对植株分化的影响;(3)高频快速建立大麦胚性悬浮细胞系.结果表明,低温预处理的较大相对湿度能明显促进花药的出愈率;低温处理愈伤组织能提高绿苗率;以7周龄的花药胚性愈伤组织为起始材料高频快速建立了均质、分散性好的胚性悬浮细胞系,建立频率约为25%～50%;已8～9周龄的花药胚性愈伤组织作为起始材料,难以建立胚性悬浮细胞系....

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Bibliographic Details
Main Authors: PANJian-wei(潘建伟), WANGWei-jun(王卫军), PANWei-huai(潘伟槐), ZHUMu-yuan(朱睦元)
Format: Article
Language:zho
Published: Zhejiang University Press 2002-07-01
Series:Zhejiang Daxue xuebao. Lixue ban
Subjects:
大麦
花药培养
胚性悬浮细胞系
Online Access:https://doi.org/zjup/1008-9497.2002.29.4.454-458

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