Hydroalcoholic extract of Glycyrrhiza glabra root combined with Linum usitatissimum oil as an alternative for hormone replacement therapy in ovariectomized rats
Objective: Plant-derived estrogens (phytoestrogens) with structural similarity to primary female sex hormones could be suitable replacements for sex hormones. Therefore, the effects of the licorice root extract and Linum usitatissimum oil on biochemical and hormonal indices in the serum and uterine...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023027640 |
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| author | Nader Tanideh Fatemeh Daneshmand Marzieh Karimimanesh Javad Mottaghipisheh Farhad Koohpeyma Omid Koohi-Hosseinabadi Romina Tanideh Cambyz Irajie Aida Iraji |
| author_facet | Nader Tanideh Fatemeh Daneshmand Marzieh Karimimanesh Javad Mottaghipisheh Farhad Koohpeyma Omid Koohi-Hosseinabadi Romina Tanideh Cambyz Irajie Aida Iraji |
| author_sort | Nader Tanideh |
| collection | DOAJ |
| description | Objective: Plant-derived estrogens (phytoestrogens) with structural similarity to primary female sex hormones could be suitable replacements for sex hormones. Therefore, the effects of the licorice root extract and Linum usitatissimum oil on biochemical and hormonal indices in the serum and uterine stereological changes in ovariectomized rats were evaluated. Design: In this study, 70 adult female rats were randomly divided into seven groups including 1) control group, 2) sham-operated group, 3) ovariectomized (OVX) group, 4) OVX rats that received 1 mg/kg estradiol for 8 weeks at the day of post-operation, 5) OVX rats which received 2.0 mg/kg body wt Linum usitatissimum oil for 8 weeks at the day of post-operation, 6) OVX rats which received 2.0 mg/kg body wt licorice extract for 8 weeks at the day of post-operation, and 7) OVX rats which received 2.0 mg/kg body wt Linum usitatissimum oil + 2.0 mg/kg body wt licorice extract for 8 weeks at the day of post-operation. After eight weeks, alkaline phosphatase activity, as well as calcium, estradiol, and progesterone concentrations were assessed and tissue samples of the uterus were serologically examined. Results: The results indicated that after 8 weeks of OVX the alkaline phosphatase activity (Mean = 637.7 IU/L) increased and the calcium (Mean = 7.09 mg/dl), estradiol (5.30 pmol/L), and progesterone (Mean = 3.53 nmol/L) reduced compared to other groups. Moreover, stereological changes in the uterus in ovariectomy groups were seen compared to the other groups. The treatment with Linum usitatissimum oil and licorice extract had a significant therapeutic effect on biochemical factors and stereological changes compared to the ovariectomized group. Conclusion: The results of this study showed that the combination of Linum usitatissimum oil with licorice extract showed the high potential of hormone replacement therapy in the reduction of OVX complications. |
| first_indexed | 2024-03-13T08:27:00Z |
| format | Article |
| id | doaj.art-9d5ae40eca7543b0a02584ed9560d462 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T08:27:00Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-9d5ae40eca7543b0a02584ed9560d4622023-05-31T04:44:45ZengElsevierHeliyon2405-84402023-05-0195e15557Hydroalcoholic extract of Glycyrrhiza glabra root combined with Linum usitatissimum oil as an alternative for hormone replacement therapy in ovariectomized ratsNader Tanideh0Fatemeh Daneshmand1Marzieh Karimimanesh2Javad Mottaghipisheh3Farhad Koohpeyma4Omid Koohi-Hosseinabadi5Romina Tanideh6Cambyz Irajie7Aida Iraji8Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Biochemistry, Payame Noor University, Taft, Yazd, IranDepartment of Biochemistry, Payame Noor University, Taft, Yazd, IranStem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranEndocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, IranLaparoscopy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, IranStem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran; Corresponding author.Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran; Corresponding author. Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Objective: Plant-derived estrogens (phytoestrogens) with structural similarity to primary female sex hormones could be suitable replacements for sex hormones. Therefore, the effects of the licorice root extract and Linum usitatissimum oil on biochemical and hormonal indices in the serum and uterine stereological changes in ovariectomized rats were evaluated. Design: In this study, 70 adult female rats were randomly divided into seven groups including 1) control group, 2) sham-operated group, 3) ovariectomized (OVX) group, 4) OVX rats that received 1 mg/kg estradiol for 8 weeks at the day of post-operation, 5) OVX rats which received 2.0 mg/kg body wt Linum usitatissimum oil for 8 weeks at the day of post-operation, 6) OVX rats which received 2.0 mg/kg body wt licorice extract for 8 weeks at the day of post-operation, and 7) OVX rats which received 2.0 mg/kg body wt Linum usitatissimum oil + 2.0 mg/kg body wt licorice extract for 8 weeks at the day of post-operation. After eight weeks, alkaline phosphatase activity, as well as calcium, estradiol, and progesterone concentrations were assessed and tissue samples of the uterus were serologically examined. Results: The results indicated that after 8 weeks of OVX the alkaline phosphatase activity (Mean = 637.7 IU/L) increased and the calcium (Mean = 7.09 mg/dl), estradiol (5.30 pmol/L), and progesterone (Mean = 3.53 nmol/L) reduced compared to other groups. Moreover, stereological changes in the uterus in ovariectomy groups were seen compared to the other groups. The treatment with Linum usitatissimum oil and licorice extract had a significant therapeutic effect on biochemical factors and stereological changes compared to the ovariectomized group. Conclusion: The results of this study showed that the combination of Linum usitatissimum oil with licorice extract showed the high potential of hormone replacement therapy in the reduction of OVX complications.http://www.sciencedirect.com/science/article/pii/S2405844023027640OvariectomyLinum usitatissimum oilGlycyrrhiza glabraStereologyUterus |
| spellingShingle | Nader Tanideh Fatemeh Daneshmand Marzieh Karimimanesh Javad Mottaghipisheh Farhad Koohpeyma Omid Koohi-Hosseinabadi Romina Tanideh Cambyz Irajie Aida Iraji Hydroalcoholic extract of Glycyrrhiza glabra root combined with Linum usitatissimum oil as an alternative for hormone replacement therapy in ovariectomized rats Heliyon Ovariectomy Linum usitatissimum oil Glycyrrhiza glabra Stereology Uterus |
| title | Hydroalcoholic extract of Glycyrrhiza glabra root combined with Linum usitatissimum oil as an alternative for hormone replacement therapy in ovariectomized rats |
| title_full | Hydroalcoholic extract of Glycyrrhiza glabra root combined with Linum usitatissimum oil as an alternative for hormone replacement therapy in ovariectomized rats |
| title_fullStr | Hydroalcoholic extract of Glycyrrhiza glabra root combined with Linum usitatissimum oil as an alternative for hormone replacement therapy in ovariectomized rats |
| title_full_unstemmed | Hydroalcoholic extract of Glycyrrhiza glabra root combined with Linum usitatissimum oil as an alternative for hormone replacement therapy in ovariectomized rats |
| title_short | Hydroalcoholic extract of Glycyrrhiza glabra root combined with Linum usitatissimum oil as an alternative for hormone replacement therapy in ovariectomized rats |
| title_sort | hydroalcoholic extract of glycyrrhiza glabra root combined with linum usitatissimum oil as an alternative for hormone replacement therapy in ovariectomized rats |
| topic | Ovariectomy Linum usitatissimum oil Glycyrrhiza glabra Stereology Uterus |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023027640 |
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