Comparison of 2- and 3-Dimensional Cultured Periodontal Ligament Stem Cells; a Pilot Study
This study compared the characteristics of periodontal ligament stem cells (PDLSCs) cultured using 3-dimensional (3D) versus conventional 2-dimensional (2D) methods. PDLSCs were cultured in either a 3D culture with a non-adhesive culture plate (Stemfit 3D<sup>®</sup>) or a conventional 2...
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MDPI AG
2021-01-01
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| Online Access: | https://www.mdpi.com/2076-3417/11/3/1083 |
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| author | Yun Yeong Jeong Mi Sun Kim Ko Eun Lee Ok Hyung Nam Ji-Hyun Jang Sung-Chul Choi Hyo-Seol Lee |
| author_facet | Yun Yeong Jeong Mi Sun Kim Ko Eun Lee Ok Hyung Nam Ji-Hyun Jang Sung-Chul Choi Hyo-Seol Lee |
| author_sort | Yun Yeong Jeong |
| collection | DOAJ |
| description | This study compared the characteristics of periodontal ligament stem cells (PDLSCs) cultured using 3-dimensional (3D) versus conventional 2-dimensional (2D) methods. PDLSCs were cultured in either a 3D culture with a non-adhesive culture plate (Stemfit 3D<sup>®</sup>) or a conventional 2D culture using a 6-well plate. Morphology, viability, proliferation ability, and osteogenic differentiation were analyzed to characterize the differences induced in identical PDLSCs by 3D and 2D culture environments. In addition, gene expression was analyzed using RNA sequencing to further characterize the functional differences. The diameter and the viability of the 3D-cultured PDLSCs decreased over time, but the shape of the spheroid was maintained for 20 days. Although osteogenic differentiation occurred in both the 2D- and 3D-cultured PDLSCs, compared to the control group it was 20.8 and 1.6 higher in the 3D- and 2D-cultured cells, respectively. RNA sequencing revealed that PDLSCs cultured using 2D and 3D methods have different gene expression profiles. The viability of the 3D-cultured cells was decreased, but they showed superior osteogenic differentiation compared to 2D-cultured cells. Within the limitations of this study, the results demonstrate that the structure and function of PDLSCs are influenced by the cell culture method. |
| first_indexed | 2024-03-09T03:41:39Z |
| format | Article |
| id | doaj.art-9d608848dd364def872e333fc0fd3766 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T03:41:39Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-9d608848dd364def872e333fc0fd37662023-12-03T14:39:26ZengMDPI AGApplied Sciences2076-34172021-01-01113108310.3390/app11031083Comparison of 2- and 3-Dimensional Cultured Periodontal Ligament Stem Cells; a Pilot StudyYun Yeong Jeong0Mi Sun Kim1Ko Eun Lee2Ok Hyung Nam3Ji-Hyun Jang4Sung-Chul Choi5Hyo-Seol Lee6Department of Pediatric Dentistry, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul 02447, KoreaDepartment of Pediatric Dentistry, Kyung Hee University Dental Hospital, Seoul 02447, KoreaDepartment of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul 02447, KoreaDepartment of Conservative Dentistry, Kyung Hee University School of Dentistry, Seoul 02447, KoreaDepartment of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul 02447, KoreaDepartment of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul 02447, KoreaThis study compared the characteristics of periodontal ligament stem cells (PDLSCs) cultured using 3-dimensional (3D) versus conventional 2-dimensional (2D) methods. PDLSCs were cultured in either a 3D culture with a non-adhesive culture plate (Stemfit 3D<sup>®</sup>) or a conventional 2D culture using a 6-well plate. Morphology, viability, proliferation ability, and osteogenic differentiation were analyzed to characterize the differences induced in identical PDLSCs by 3D and 2D culture environments. In addition, gene expression was analyzed using RNA sequencing to further characterize the functional differences. The diameter and the viability of the 3D-cultured PDLSCs decreased over time, but the shape of the spheroid was maintained for 20 days. Although osteogenic differentiation occurred in both the 2D- and 3D-cultured PDLSCs, compared to the control group it was 20.8 and 1.6 higher in the 3D- and 2D-cultured cells, respectively. RNA sequencing revealed that PDLSCs cultured using 2D and 3D methods have different gene expression profiles. The viability of the 3D-cultured cells was decreased, but they showed superior osteogenic differentiation compared to 2D-cultured cells. Within the limitations of this study, the results demonstrate that the structure and function of PDLSCs are influenced by the cell culture method.https://www.mdpi.com/2076-3417/11/3/1083periodontal ligament stem cell3-dimensional culturespheroidmorphologyosteogenic differentiationRNA sequencing |
| spellingShingle | Yun Yeong Jeong Mi Sun Kim Ko Eun Lee Ok Hyung Nam Ji-Hyun Jang Sung-Chul Choi Hyo-Seol Lee Comparison of 2- and 3-Dimensional Cultured Periodontal Ligament Stem Cells; a Pilot Study Applied Sciences periodontal ligament stem cell 3-dimensional culture spheroid morphology osteogenic differentiation RNA sequencing |
| title | Comparison of 2- and 3-Dimensional Cultured Periodontal Ligament Stem Cells; a Pilot Study |
| title_full | Comparison of 2- and 3-Dimensional Cultured Periodontal Ligament Stem Cells; a Pilot Study |
| title_fullStr | Comparison of 2- and 3-Dimensional Cultured Periodontal Ligament Stem Cells; a Pilot Study |
| title_full_unstemmed | Comparison of 2- and 3-Dimensional Cultured Periodontal Ligament Stem Cells; a Pilot Study |
| title_short | Comparison of 2- and 3-Dimensional Cultured Periodontal Ligament Stem Cells; a Pilot Study |
| title_sort | comparison of 2 and 3 dimensional cultured periodontal ligament stem cells a pilot study |
| topic | periodontal ligament stem cell 3-dimensional culture spheroid morphology osteogenic differentiation RNA sequencing |
| url | https://www.mdpi.com/2076-3417/11/3/1083 |
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