Sporozoite glycoprotein antigen (SGA) of Cryptosporidium parvum is a promising vaccine target for prevention of cryptosporidiosis In silico analysis of C. parvum SGA protein
Cryptosporidiosis is a zoonotic parasitic infection causing diarrhea in humans and farm animals. The present study was done to computationally analyze the Cryptosporidium parvum sporozoite glycoprotein antigen (SGA) regarding immunogenic epitopes, as a potential vaccine candidate. Physico-chemical p...
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| Format: | Article |
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Elsevier
2023-01-01
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| Series: | Informatics in Medicine Unlocked |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235291482300179X |
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| author | Bahareh Kordi Amir Fotovati Hamidreza Majidiani Hadi Fazel Esfandiar Azizi Morteza Shams Amir Abaszadeh |
| author_facet | Bahareh Kordi Amir Fotovati Hamidreza Majidiani Hadi Fazel Esfandiar Azizi Morteza Shams Amir Abaszadeh |
| author_sort | Bahareh Kordi |
| collection | DOAJ |
| description | Cryptosporidiosis is a zoonotic parasitic infection causing diarrhea in humans and farm animals. The present study was done to computationally analyze the Cryptosporidium parvum sporozoite glycoprotein antigen (SGA) regarding immunogenic epitopes, as a potential vaccine candidate. Physico-chemical properties, solubility, antigenicity, allergenicity, signal peptide, transmembrane domain and post-translational modifications (PTMs) along with secondary and tertiary structure analyses were predicted. Also, linear B-cell epitopes, cytotoxic T-lymphocyte (CTL; human, cattle) and helper T-lymphocyte (HTL; human) epitopes were forecasted. The molecular weight of this 265-residue protein was 27.14 kDa, with hydrophilicity (GRAVY: 0.700), high solubility (0.892), and aliphatic index (46.83). No signal peptide and transmembrane domain was predicted, and the most abundant PTMs were phosphorylation (n = 68) and O-glycosylation (n = 53). Coils were dominant in secondary structure analysis, and the tertiary model refinement showed enhancement in the refined than crude model. Only 4 shared B-cell epitopes among three web servers (ABCpred, BepiPred 2.0, and SVMTriP) were shown to be antigenic, non-allergenic, with good water solubility. Also, five potent CTL epitopes in humans and two in cattle were predicted. Notably, four HTL epitopes were found as inducers of IFN-γ, IL-4 or both. In conclusion, our results demonstrated several immunogenic epitopes in this protein, which could be directed towards multi-epitope vaccine design. |
| first_indexed | 2024-03-11T15:04:56Z |
| format | Article |
| id | doaj.art-9d631861a5404693b3bed2df4728cca7 |
| institution | Directory Open Access Journal |
| issn | 2352-9148 |
| language | English |
| last_indexed | 2024-03-11T15:04:56Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Informatics in Medicine Unlocked |
| spelling | doaj.art-9d631861a5404693b3bed2df4728cca72023-10-30T06:05:08ZengElsevierInformatics in Medicine Unlocked2352-91482023-01-0142101333Sporozoite glycoprotein antigen (SGA) of Cryptosporidium parvum is a promising vaccine target for prevention of cryptosporidiosis In silico analysis of C. parvum SGA proteinBahareh Kordi0Amir Fotovati1Hamidreza Majidiani2Hadi Fazel3Esfandiar Azizi4Morteza Shams5Amir Abaszadeh6Department of Agricultural Science, Technical and Vocational University (TVU), Tehran, IranFaculty of Veterinary Medicine, University of Zabol, Zabol, IranHealthy Aging Research Centre, Neyshabur University of Medical Sciences, Neyshabur, Iran; Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, IranDepartment of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, IranDepartment of Immunology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IranZoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran; Corresponding author.Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran; School of Medicine, Ilam University of Medical Sciences, Ilam, IranCryptosporidiosis is a zoonotic parasitic infection causing diarrhea in humans and farm animals. The present study was done to computationally analyze the Cryptosporidium parvum sporozoite glycoprotein antigen (SGA) regarding immunogenic epitopes, as a potential vaccine candidate. Physico-chemical properties, solubility, antigenicity, allergenicity, signal peptide, transmembrane domain and post-translational modifications (PTMs) along with secondary and tertiary structure analyses were predicted. Also, linear B-cell epitopes, cytotoxic T-lymphocyte (CTL; human, cattle) and helper T-lymphocyte (HTL; human) epitopes were forecasted. The molecular weight of this 265-residue protein was 27.14 kDa, with hydrophilicity (GRAVY: 0.700), high solubility (0.892), and aliphatic index (46.83). No signal peptide and transmembrane domain was predicted, and the most abundant PTMs were phosphorylation (n = 68) and O-glycosylation (n = 53). Coils were dominant in secondary structure analysis, and the tertiary model refinement showed enhancement in the refined than crude model. Only 4 shared B-cell epitopes among three web servers (ABCpred, BepiPred 2.0, and SVMTriP) were shown to be antigenic, non-allergenic, with good water solubility. Also, five potent CTL epitopes in humans and two in cattle were predicted. Notably, four HTL epitopes were found as inducers of IFN-γ, IL-4 or both. In conclusion, our results demonstrated several immunogenic epitopes in this protein, which could be directed towards multi-epitope vaccine design.http://www.sciencedirect.com/science/article/pii/S235291482300179XCryptosporidium parvumSGAMulti-epitope vaccine |
| spellingShingle | Bahareh Kordi Amir Fotovati Hamidreza Majidiani Hadi Fazel Esfandiar Azizi Morteza Shams Amir Abaszadeh Sporozoite glycoprotein antigen (SGA) of Cryptosporidium parvum is a promising vaccine target for prevention of cryptosporidiosis In silico analysis of C. parvum SGA protein Informatics in Medicine Unlocked Cryptosporidium parvum SGA Multi-epitope vaccine |
| title | Sporozoite glycoprotein antigen (SGA) of Cryptosporidium parvum is a promising vaccine target for prevention of cryptosporidiosis In silico analysis of C. parvum SGA protein |
| title_full | Sporozoite glycoprotein antigen (SGA) of Cryptosporidium parvum is a promising vaccine target for prevention of cryptosporidiosis In silico analysis of C. parvum SGA protein |
| title_fullStr | Sporozoite glycoprotein antigen (SGA) of Cryptosporidium parvum is a promising vaccine target for prevention of cryptosporidiosis In silico analysis of C. parvum SGA protein |
| title_full_unstemmed | Sporozoite glycoprotein antigen (SGA) of Cryptosporidium parvum is a promising vaccine target for prevention of cryptosporidiosis In silico analysis of C. parvum SGA protein |
| title_short | Sporozoite glycoprotein antigen (SGA) of Cryptosporidium parvum is a promising vaccine target for prevention of cryptosporidiosis In silico analysis of C. parvum SGA protein |
| title_sort | sporozoite glycoprotein antigen sga of cryptosporidium parvum is a promising vaccine target for prevention of cryptosporidiosis in silico analysis of c parvum sga protein |
| topic | Cryptosporidium parvum SGA Multi-epitope vaccine |
| url | http://www.sciencedirect.com/science/article/pii/S235291482300179X |
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