Matrix Metalloproteinase-3 Down Regulation and Cell Migration Inhibition in Human Pterygium Fibroblasts by Mitomycin-C, Curcumin and Fibrin Glue
BACKGROUND: Pterygium is an ocular surface disease that often occurs in tropical countries with a high recurrence rate. Matrix metalloproteinase-3 (MMP-3) play a key role in the inflammatory process of pterygium. This study aims to investigate the ability of curcumin and fibrin glue (FG) in suppress...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Secretariat of The Indonesian Biomedical Journal
2022-06-01
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| Series: | Indonesian Biomedical Journal |
| Online Access: | https://inabj.org/index.php/ibj/article/view/1825 |
| _version_ | 1811237291033624576 |
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| author | Ferdian Ramadhan Jamaluddin Jamaluddin Ismi Zuhria Luki Indriaswati Evelyn Komaratih |
| author_facet | Ferdian Ramadhan Jamaluddin Jamaluddin Ismi Zuhria Luki Indriaswati Evelyn Komaratih |
| author_sort | Ferdian Ramadhan |
| collection | DOAJ |
| description | BACKGROUND: Pterygium is an ocular surface disease that often occurs in tropical countries with a high recurrence rate. Matrix metalloproteinase-3 (MMP-3) play a key role in the inflammatory process of pterygium. This study aims to investigate the ability of curcumin and fibrin glue (FG) in suppressing the expression of MMP-3, and whether can be expected as adjuvant therapy to reduce pterygium recurrence.
METHODS: Human pterygium fibroblasts (HPF) obtained from primary cultured of pterygium were treated with no treatment, curcumin, mitomycin-C (MMC), and FG. MMP-3 expression was analyzed using immunocytochemistry and the intensity measurement was done using ImageJ software. Cell migration was measured by scratching and stratification of fibroblast culture after cell confluence, and assessed for 48 hours.
RESULTS: The expression of MMP-3 were lower in the HPF treated with 100 mol/mL curcumin, 200 mol/mL, and FG (2205.84±86.1 pg/mL, 1002.51±25.22 pg/mL, 1131.55±17.71 pg/mL, respectively) in comparison with untreated HPF (4703.49±108.9 pg/mL). The expression of MMP-3 were significantly different between groups (p<0.001). Cell migration of HPF after scratching with curcumin intervention at 200 mol/mL decrease from 178.67±2.85 (24 hours) to 88.83±1.48 (48 hours). Meanwhile the migration in FG group also decrease from 180.4±2.56 (24 hours) to 72.45±1.25 (48 hours).
CONCLUSION: Curcumin and FG able to reduce the expression of MMP-3 and inhibit the migration of HPF cells.
KEYWORDS: curcumin, mitomycin C, fibrin glue, human pterygium fibroblast, MMP-3 |
| first_indexed | 2024-04-12T12:22:44Z |
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| id | doaj.art-9d633c5ce0e044db902a29305b50bfef |
| institution | Directory Open Access Journal |
| issn | 2085-3297 2355-9179 |
| language | English |
| last_indexed | 2024-04-12T12:22:44Z |
| publishDate | 2022-06-01 |
| publisher | Secretariat of The Indonesian Biomedical Journal |
| record_format | Article |
| series | Indonesian Biomedical Journal |
| spelling | doaj.art-9d633c5ce0e044db902a29305b50bfef2022-12-22T03:33:15ZengSecretariat of The Indonesian Biomedical JournalIndonesian Biomedical Journal2085-32972355-91792022-06-01142172910.18585/inabj.v14i2.1825458Matrix Metalloproteinase-3 Down Regulation and Cell Migration Inhibition in Human Pterygium Fibroblasts by Mitomycin-C, Curcumin and Fibrin GlueFerdian Ramadhan0Jamaluddin Jamaluddin1Ismi Zuhria2Luki Indriaswati3Evelyn Komaratih4Ophthalmology Resident, Faculty of Medicine, Universitas Airlangga/Dr.Soetomo General Hospital, Jl. Mayjen. Prof. Dr. Moestopo No. 47, SurabayaRumah Sakit Mata Masyarakat Jawa Timur, Jl. Ketintang Baru Sel. I No.1, Surabaya 60232Department of Ophthalmology, Faculty of Medicine, Universitas Airlangga/Dr.Soetomo General Hospital, Jl. Mayjen. Prof. Dr. Moestopo No. 47, SurabayaDepartment of Ophthalmology, Faculty of Medicine, Universitas Airlangga/Dr.Soetomo General Hospital, Jl. Mayjen. Prof. Dr. Moestopo No. 47, SurabayaDepartment of Ophthalmology, Faculty of Medicine, Universitas Airlangga/Dr.Soetomo General Hospital, Jl. Mayjen. Prof. Dr. Moestopo No. 47, SurabayaBACKGROUND: Pterygium is an ocular surface disease that often occurs in tropical countries with a high recurrence rate. Matrix metalloproteinase-3 (MMP-3) play a key role in the inflammatory process of pterygium. This study aims to investigate the ability of curcumin and fibrin glue (FG) in suppressing the expression of MMP-3, and whether can be expected as adjuvant therapy to reduce pterygium recurrence. METHODS: Human pterygium fibroblasts (HPF) obtained from primary cultured of pterygium were treated with no treatment, curcumin, mitomycin-C (MMC), and FG. MMP-3 expression was analyzed using immunocytochemistry and the intensity measurement was done using ImageJ software. Cell migration was measured by scratching and stratification of fibroblast culture after cell confluence, and assessed for 48 hours. RESULTS: The expression of MMP-3 were lower in the HPF treated with 100 mol/mL curcumin, 200 mol/mL, and FG (2205.84±86.1 pg/mL, 1002.51±25.22 pg/mL, 1131.55±17.71 pg/mL, respectively) in comparison with untreated HPF (4703.49±108.9 pg/mL). The expression of MMP-3 were significantly different between groups (p<0.001). Cell migration of HPF after scratching with curcumin intervention at 200 mol/mL decrease from 178.67±2.85 (24 hours) to 88.83±1.48 (48 hours). Meanwhile the migration in FG group also decrease from 180.4±2.56 (24 hours) to 72.45±1.25 (48 hours). CONCLUSION: Curcumin and FG able to reduce the expression of MMP-3 and inhibit the migration of HPF cells. KEYWORDS: curcumin, mitomycin C, fibrin glue, human pterygium fibroblast, MMP-3https://inabj.org/index.php/ibj/article/view/1825 |
| spellingShingle | Ferdian Ramadhan Jamaluddin Jamaluddin Ismi Zuhria Luki Indriaswati Evelyn Komaratih Matrix Metalloproteinase-3 Down Regulation and Cell Migration Inhibition in Human Pterygium Fibroblasts by Mitomycin-C, Curcumin and Fibrin Glue Indonesian Biomedical Journal |
| title | Matrix Metalloproteinase-3 Down Regulation and Cell Migration Inhibition in Human Pterygium Fibroblasts by Mitomycin-C, Curcumin and Fibrin Glue |
| title_full | Matrix Metalloproteinase-3 Down Regulation and Cell Migration Inhibition in Human Pterygium Fibroblasts by Mitomycin-C, Curcumin and Fibrin Glue |
| title_fullStr | Matrix Metalloproteinase-3 Down Regulation and Cell Migration Inhibition in Human Pterygium Fibroblasts by Mitomycin-C, Curcumin and Fibrin Glue |
| title_full_unstemmed | Matrix Metalloproteinase-3 Down Regulation and Cell Migration Inhibition in Human Pterygium Fibroblasts by Mitomycin-C, Curcumin and Fibrin Glue |
| title_short | Matrix Metalloproteinase-3 Down Regulation and Cell Migration Inhibition in Human Pterygium Fibroblasts by Mitomycin-C, Curcumin and Fibrin Glue |
| title_sort | matrix metalloproteinase 3 down regulation and cell migration inhibition in human pterygium fibroblasts by mitomycin c curcumin and fibrin glue |
| url | https://inabj.org/index.php/ibj/article/view/1825 |
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