Antimicrobial and antibiofilm potentials of cinnamon oil and silver nanoparticles against Streptococcus agalactiae isolated from bovine mastitis: new avenues for countering resistance
Abstract Background Streptococcus agalactiae (S. agalactiae) is a contagious pathogen of bovine mastitis. It has financial implications for the dairy cattle industry in certain areas of the world. Since antimicrobial resistance increases in dairy farms, natural antimicrobials from herbal origins and...
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| Format: | Article |
| Language: | English |
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BMC
2021-03-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-021-02842-9 |
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| author | Norhan K. Abd El-Aziz Ahmed M. Ammar El-sayed Y. M. El-Naenaeey Hend M. El Damaty Asmaa A. Elazazy Ahmed A. Hefny Asmaa Shaker Ibrahim E. Eldesoukey |
| author_facet | Norhan K. Abd El-Aziz Ahmed M. Ammar El-sayed Y. M. El-Naenaeey Hend M. El Damaty Asmaa A. Elazazy Ahmed A. Hefny Asmaa Shaker Ibrahim E. Eldesoukey |
| author_sort | Norhan K. Abd El-Aziz |
| collection | DOAJ |
| description | Abstract Background Streptococcus agalactiae (S. agalactiae) is a contagious pathogen of bovine mastitis. It has financial implications for the dairy cattle industry in certain areas of the world. Since antimicrobial resistance increases in dairy farms, natural antimicrobials from herbal origins and nanoparticles have been given more attention as an alternative therapy. Hence, this study reported the antimicrobial and antibiofilm potentials of cinnamon oil, silver nanoparticles (AgNPs), and their combination against multidrug-resistant (MDR) S. agalactiae recovered from clinical bovine mastitis in Egypt. Results Our findings revealed that 73% (146/200) of the examined milk samples collected from dairy cows with clinical mastitis were infected with Streptococci species. Of these, 9.59% (14/146) were identified as S. agalactiae and categorized as MDR. S. agalactiae isolates expressed four virulence genes (Hyl, cylE, scpB, and lmb) and demonstrated an ability to produce biofilms. Cinnamon oil showed high antimicrobial (MICs ≤0.063 μg /mL) and antibiofilm (MBIC50 = 4 μg/mL) potentials against planktonic and biofilms of S. agalactiae isolates, respectively. However, AgNPs showed reasonable antimicrobial (MICs ≤16 μg/mL) and relatively low antibiofilm (MBIC50 = 64 μg/mL) activities against screened isolates. Synergistic antimicrobial or additive antibiofilm interactions of cinnamon oil combined with AgNPs were reported for the first time. Scanning electron microscope (SEM) analysis revealed that biofilms of S. agalactiae isolates treated with cinnamon oil were more seriously damaged than observed in AgNPs cinnamon oil combination. Moreover, reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) showed that cinnamon oil exerted a remarkable down-regulation of pili biosynthesis genes (pilA and pilB) and their regulator (rogB) against S. agalactiae biofilms, meanwhile the AgNPs cinnamon oil combination demonstrated a lower efficacy. Conclusions This is an in vitro preliminary approach that documented the antibiofilm potential of cinnamon oil and the inhibitory activity of cinnamon oil and its combination with AgNPs against MDR S. agalactiae recovered from clinical mastitis. Further in vivo studies should be carried out in animal models to provide evidence of concept for implementing these alternative candidates in the treatment of dairy farms infected by streptococcal mastitis in the future. |
| first_indexed | 2024-12-20T07:58:36Z |
| format | Article |
| id | doaj.art-9d640125aa2a439585b3227a7006c40c |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-20T07:58:36Z |
| publishDate | 2021-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-9d640125aa2a439585b3227a7006c40c2022-12-21T19:47:35ZengBMCBMC Veterinary Research1746-61482021-03-0117111410.1186/s12917-021-02842-9Antimicrobial and antibiofilm potentials of cinnamon oil and silver nanoparticles against Streptococcus agalactiae isolated from bovine mastitis: new avenues for countering resistanceNorhan K. Abd El-Aziz0Ahmed M. Ammar1El-sayed Y. M. El-Naenaeey2Hend M. El Damaty3Asmaa A. Elazazy4Ahmed A. Hefny5Asmaa Shaker6Ibrahim E. Eldesoukey7Department of Microbiology, Faculty of Veterinary Medicine, Zagazig UniversityDepartment of Microbiology, Faculty of Veterinary Medicine, Zagazig UniversityDepartment of Microbiology, Faculty of Veterinary Medicine, Zagazig UniversityDepartment of Animal Medicine, Infectious Diseases, Faculty of Veterinary Medicine, Zagazig UniversityAbou Hamad Veterinary Organizations, Ministry of AgricultureVeterinary Hospital, Faculty of Veterinary Medicine, Zagazig UniversityDepartment of Microbiology, Veterinary Hospital, Faculty of Veterinary Medicine, University of Sadat CityDepartment of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh UniversityAbstract Background Streptococcus agalactiae (S. agalactiae) is a contagious pathogen of bovine mastitis. It has financial implications for the dairy cattle industry in certain areas of the world. Since antimicrobial resistance increases in dairy farms, natural antimicrobials from herbal origins and nanoparticles have been given more attention as an alternative therapy. Hence, this study reported the antimicrobial and antibiofilm potentials of cinnamon oil, silver nanoparticles (AgNPs), and their combination against multidrug-resistant (MDR) S. agalactiae recovered from clinical bovine mastitis in Egypt. Results Our findings revealed that 73% (146/200) of the examined milk samples collected from dairy cows with clinical mastitis were infected with Streptococci species. Of these, 9.59% (14/146) were identified as S. agalactiae and categorized as MDR. S. agalactiae isolates expressed four virulence genes (Hyl, cylE, scpB, and lmb) and demonstrated an ability to produce biofilms. Cinnamon oil showed high antimicrobial (MICs ≤0.063 μg /mL) and antibiofilm (MBIC50 = 4 μg/mL) potentials against planktonic and biofilms of S. agalactiae isolates, respectively. However, AgNPs showed reasonable antimicrobial (MICs ≤16 μg/mL) and relatively low antibiofilm (MBIC50 = 64 μg/mL) activities against screened isolates. Synergistic antimicrobial or additive antibiofilm interactions of cinnamon oil combined with AgNPs were reported for the first time. Scanning electron microscope (SEM) analysis revealed that biofilms of S. agalactiae isolates treated with cinnamon oil were more seriously damaged than observed in AgNPs cinnamon oil combination. Moreover, reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) showed that cinnamon oil exerted a remarkable down-regulation of pili biosynthesis genes (pilA and pilB) and their regulator (rogB) against S. agalactiae biofilms, meanwhile the AgNPs cinnamon oil combination demonstrated a lower efficacy. Conclusions This is an in vitro preliminary approach that documented the antibiofilm potential of cinnamon oil and the inhibitory activity of cinnamon oil and its combination with AgNPs against MDR S. agalactiae recovered from clinical mastitis. Further in vivo studies should be carried out in animal models to provide evidence of concept for implementing these alternative candidates in the treatment of dairy farms infected by streptococcal mastitis in the future.https://doi.org/10.1186/s12917-021-02842-9MastitisCinnamon oilSilver nanoparticlesAntimicrobial activityAntibiofilm potential |
| spellingShingle | Norhan K. Abd El-Aziz Ahmed M. Ammar El-sayed Y. M. El-Naenaeey Hend M. El Damaty Asmaa A. Elazazy Ahmed A. Hefny Asmaa Shaker Ibrahim E. Eldesoukey Antimicrobial and antibiofilm potentials of cinnamon oil and silver nanoparticles against Streptococcus agalactiae isolated from bovine mastitis: new avenues for countering resistance BMC Veterinary Research Mastitis Cinnamon oil Silver nanoparticles Antimicrobial activity Antibiofilm potential |
| title | Antimicrobial and antibiofilm potentials of cinnamon oil and silver nanoparticles against Streptococcus agalactiae isolated from bovine mastitis: new avenues for countering resistance |
| title_full | Antimicrobial and antibiofilm potentials of cinnamon oil and silver nanoparticles against Streptococcus agalactiae isolated from bovine mastitis: new avenues for countering resistance |
| title_fullStr | Antimicrobial and antibiofilm potentials of cinnamon oil and silver nanoparticles against Streptococcus agalactiae isolated from bovine mastitis: new avenues for countering resistance |
| title_full_unstemmed | Antimicrobial and antibiofilm potentials of cinnamon oil and silver nanoparticles against Streptococcus agalactiae isolated from bovine mastitis: new avenues for countering resistance |
| title_short | Antimicrobial and antibiofilm potentials of cinnamon oil and silver nanoparticles against Streptococcus agalactiae isolated from bovine mastitis: new avenues for countering resistance |
| title_sort | antimicrobial and antibiofilm potentials of cinnamon oil and silver nanoparticles against streptococcus agalactiae isolated from bovine mastitis new avenues for countering resistance |
| topic | Mastitis Cinnamon oil Silver nanoparticles Antimicrobial activity Antibiofilm potential |
| url | https://doi.org/10.1186/s12917-021-02842-9 |
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