Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress response
ABSTRACT: Objectives: Acanthamoeba keratitis is a severe corneal infection caused by a ubiquitous opportunistic protozoan pathogen known as acanthamoeba. For the last decade, the approach to treating this infection typically includes the use of polyhexamethylene biguanide (0.02%) and/or chlorhexidi...
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Elsevier
2022-09-01
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Series: | Journal of Global Antimicrobial Resistance |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716522001205 |
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author | Ines Sifaoui Ikrame Zeouk María Reyes-Batlle Atteneri Lopez-Arencibia José E. Piñero Jacob Lorenzo-Morales |
author_facet | Ines Sifaoui Ikrame Zeouk María Reyes-Batlle Atteneri Lopez-Arencibia José E. Piñero Jacob Lorenzo-Morales |
author_sort | Ines Sifaoui |
collection | DOAJ |
description | ABSTRACT: Objectives: Acanthamoeba keratitis is a severe corneal infection caused by a ubiquitous opportunistic protozoan pathogen known as acanthamoeba. For the last decade, the approach to treating this infection typically includes the use of polyhexamethylene biguanide (0.02%) and/or chlorhexidine (Chx) (0.02%). Although chlorhexidine is reportedly effective, its mode of action towards this type of cell is not clear. The aim of this work was to study the effect of chlorhexidine on the oxidative status of Acanthamoeba polyphaga. Methods: The effect of chlorhexidine (Chx) on the oxidative state of Acanthamoeba polyphaga was studied using different antiradical methods including ABTS, DPPH and FRAP and measuring the activity of a couple of antioxidant enzyme namely SOD, NADH-FRD and SDH. Results: The chlorhexidine was able to induce oxidative imbalance in cells by over expression of reactive oxygen species and/or inhibiting the antioxidant enzymes. In addition to enhancing the antiradical activity in response to oxidative stress, the present drug was able to reduce the activity of two antioxidant enzymes, superoxide dismutase (SOD) and reduced flavin adenine dinucleotide-fumarate reductase (NADH-FRD), to 30% and 40%, respectively. Conclusions: We could observe an increase of the antiradical capacity of cell’s lysate supernatant, to cope with the overproduction of ROS. The imbalance state The inhibition of both SOD and NADH-FRD activities could have a major role in cell oxidative imbalance. |
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last_indexed | 2024-12-10T11:14:33Z |
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series | Journal of Global Antimicrobial Resistance |
spelling | doaj.art-9d662ee6433b4777a7d5ff8888df84b22022-12-22T01:51:15ZengElsevierJournal of Global Antimicrobial Resistance2213-71652022-09-0130468473Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress responseInes Sifaoui0Ikrame Zeouk1María Reyes-Batlle2Atteneri Lopez-Arencibia3José E. Piñero4Jacob Lorenzo-Morales5Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Tenerife, Spain; Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad De La Laguna, La Laguna, Tenerife, Islas Canarias, Spain; Red de Investigación Cooperativa en Enfermedades Tropicales (RICET); Corresponding author.Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Tenerife, Spain; National Agency of Medicinal and Aromatic Plants, Laboratory of Pharmaceutical Industry, Taounate, 34025, MoroccoInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Tenerife, Spain; Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad De La Laguna, La Laguna, Tenerife, Islas Canarias, Spain; Red de Investigación Cooperativa en Enfermedades Tropicales (RICET)Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Tenerife, Spain; Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad De La Laguna, La Laguna, Tenerife, Islas Canarias, Spain; Red de Investigación Cooperativa en Enfermedades Tropicales (RICET)Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Tenerife, Spain; Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad De La Laguna, La Laguna, Tenerife, Islas Canarias, Spain; Red de Investigación Cooperativa en Enfermedades Tropicales (RICET); CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, Spain; Alternative corresponding author.Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Tenerife, Spain; Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad De La Laguna, La Laguna, Tenerife, Islas Canarias, Spain; Red de Investigación Cooperativa en Enfermedades Tropicales (RICET); CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, SpainABSTRACT: Objectives: Acanthamoeba keratitis is a severe corneal infection caused by a ubiquitous opportunistic protozoan pathogen known as acanthamoeba. For the last decade, the approach to treating this infection typically includes the use of polyhexamethylene biguanide (0.02%) and/or chlorhexidine (Chx) (0.02%). Although chlorhexidine is reportedly effective, its mode of action towards this type of cell is not clear. The aim of this work was to study the effect of chlorhexidine on the oxidative status of Acanthamoeba polyphaga. Methods: The effect of chlorhexidine (Chx) on the oxidative state of Acanthamoeba polyphaga was studied using different antiradical methods including ABTS, DPPH and FRAP and measuring the activity of a couple of antioxidant enzyme namely SOD, NADH-FRD and SDH. Results: The chlorhexidine was able to induce oxidative imbalance in cells by over expression of reactive oxygen species and/or inhibiting the antioxidant enzymes. In addition to enhancing the antiradical activity in response to oxidative stress, the present drug was able to reduce the activity of two antioxidant enzymes, superoxide dismutase (SOD) and reduced flavin adenine dinucleotide-fumarate reductase (NADH-FRD), to 30% and 40%, respectively. Conclusions: We could observe an increase of the antiradical capacity of cell’s lysate supernatant, to cope with the overproduction of ROS. The imbalance state The inhibition of both SOD and NADH-FRD activities could have a major role in cell oxidative imbalance.http://www.sciencedirect.com/science/article/pii/S2213716522001205Acanthamoeba polyphagaChlorhexidineAntioxidant activityOxidative stress |
spellingShingle | Ines Sifaoui Ikrame Zeouk María Reyes-Batlle Atteneri Lopez-Arencibia José E. Piñero Jacob Lorenzo-Morales Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress response Journal of Global Antimicrobial Resistance Acanthamoeba polyphaga Chlorhexidine Antioxidant activity Oxidative stress |
title | Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress response |
title_full | Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress response |
title_fullStr | Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress response |
title_full_unstemmed | Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress response |
title_short | Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress response |
title_sort | inhibition of acanthamoeba polyphaga by chlorhexidine mediated oxidative stress response |
topic | Acanthamoeba polyphaga Chlorhexidine Antioxidant activity Oxidative stress |
url | http://www.sciencedirect.com/science/article/pii/S2213716522001205 |
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