Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress response

ABSTRACT: Objectives: Acanthamoeba keratitis is a severe corneal infection caused by a ubiquitous opportunistic protozoan pathogen known as acanthamoeba. For the last decade, the approach to treating this infection typically includes the use of polyhexamethylene biguanide (0.02%) and/or chlorhexidi...

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Main Authors: Ines Sifaoui, Ikrame Zeouk, María Reyes-Batlle, Atteneri Lopez-Arencibia, José E. Piñero, Jacob Lorenzo-Morales
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:Journal of Global Antimicrobial Resistance
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2213716522001205
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author Ines Sifaoui
Ikrame Zeouk
María Reyes-Batlle
Atteneri Lopez-Arencibia
José E. Piñero
Jacob Lorenzo-Morales
author_facet Ines Sifaoui
Ikrame Zeouk
María Reyes-Batlle
Atteneri Lopez-Arencibia
José E. Piñero
Jacob Lorenzo-Morales
author_sort Ines Sifaoui
collection DOAJ
description ABSTRACT: Objectives: Acanthamoeba keratitis is a severe corneal infection caused by a ubiquitous opportunistic protozoan pathogen known as acanthamoeba. For the last decade, the approach to treating this infection typically includes the use of polyhexamethylene biguanide (0.02%) and/or chlorhexidine (Chx) (0.02%). Although chlorhexidine is reportedly effective, its mode of action towards this type of cell is not clear. The aim of this work was to study the effect of chlorhexidine on the oxidative status of Acanthamoeba polyphaga. Methods: The effect of chlorhexidine (Chx) on the oxidative state of Acanthamoeba polyphaga was studied using different antiradical methods including ABTS, DPPH and FRAP and measuring the activity of a couple of antioxidant enzyme namely SOD, NADH-FRD and SDH. Results: The chlorhexidine was able to induce oxidative imbalance in cells by over expression of reactive oxygen species and/or inhibiting the antioxidant enzymes. In addition to enhancing the antiradical activity in response to oxidative stress, the present drug was able to reduce the activity of two antioxidant enzymes, superoxide dismutase (SOD) and reduced flavin adenine dinucleotide-fumarate reductase (NADH-FRD), to 30% and 40%, respectively. Conclusions: We could observe an increase of the antiradical capacity of cell’s lysate supernatant, to cope with the overproduction of ROS. The imbalance state The inhibition of both SOD and NADH-FRD activities could have a major role in cell oxidative imbalance.
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spelling doaj.art-9d662ee6433b4777a7d5ff8888df84b22022-12-22T01:51:15ZengElsevierJournal of Global Antimicrobial Resistance2213-71652022-09-0130468473Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress responseInes Sifaoui0Ikrame Zeouk1María Reyes-Batlle2Atteneri Lopez-Arencibia3José E. Piñero4Jacob Lorenzo-Morales5Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Tenerife, Spain; Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad De La Laguna, La Laguna, Tenerife, Islas Canarias, Spain; Red de Investigación Cooperativa en Enfermedades Tropicales (RICET); Corresponding author.Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Tenerife, Spain; National Agency of Medicinal and Aromatic Plants, Laboratory of Pharmaceutical Industry, Taounate, 34025, MoroccoInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Tenerife, Spain; Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad De La Laguna, La Laguna, Tenerife, Islas Canarias, Spain; Red de Investigación Cooperativa en Enfermedades Tropicales (RICET)Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Tenerife, Spain; Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad De La Laguna, La Laguna, Tenerife, Islas Canarias, Spain; Red de Investigación Cooperativa en Enfermedades Tropicales (RICET)Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Tenerife, Spain; Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad De La Laguna, La Laguna, Tenerife, Islas Canarias, Spain; Red de Investigación Cooperativa en Enfermedades Tropicales (RICET); CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, Spain; Alternative corresponding author.Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Tenerife, Spain; Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad De La Laguna, La Laguna, Tenerife, Islas Canarias, Spain; Red de Investigación Cooperativa en Enfermedades Tropicales (RICET); CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, SpainABSTRACT: Objectives: Acanthamoeba keratitis is a severe corneal infection caused by a ubiquitous opportunistic protozoan pathogen known as acanthamoeba. For the last decade, the approach to treating this infection typically includes the use of polyhexamethylene biguanide (0.02%) and/or chlorhexidine (Chx) (0.02%). Although chlorhexidine is reportedly effective, its mode of action towards this type of cell is not clear. The aim of this work was to study the effect of chlorhexidine on the oxidative status of Acanthamoeba polyphaga. Methods: The effect of chlorhexidine (Chx) on the oxidative state of Acanthamoeba polyphaga was studied using different antiradical methods including ABTS, DPPH and FRAP and measuring the activity of a couple of antioxidant enzyme namely SOD, NADH-FRD and SDH. Results: The chlorhexidine was able to induce oxidative imbalance in cells by over expression of reactive oxygen species and/or inhibiting the antioxidant enzymes. In addition to enhancing the antiradical activity in response to oxidative stress, the present drug was able to reduce the activity of two antioxidant enzymes, superoxide dismutase (SOD) and reduced flavin adenine dinucleotide-fumarate reductase (NADH-FRD), to 30% and 40%, respectively. Conclusions: We could observe an increase of the antiradical capacity of cell’s lysate supernatant, to cope with the overproduction of ROS. The imbalance state The inhibition of both SOD and NADH-FRD activities could have a major role in cell oxidative imbalance.http://www.sciencedirect.com/science/article/pii/S2213716522001205Acanthamoeba polyphagaChlorhexidineAntioxidant activityOxidative stress
spellingShingle Ines Sifaoui
Ikrame Zeouk
María Reyes-Batlle
Atteneri Lopez-Arencibia
José E. Piñero
Jacob Lorenzo-Morales
Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress response
Journal of Global Antimicrobial Resistance
Acanthamoeba polyphaga
Chlorhexidine
Antioxidant activity
Oxidative stress
title Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress response
title_full Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress response
title_fullStr Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress response
title_full_unstemmed Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress response
title_short Inhibition of Acanthamoeba polyphaga by chlorhexidine-mediated oxidative stress response
title_sort inhibition of acanthamoeba polyphaga by chlorhexidine mediated oxidative stress response
topic Acanthamoeba polyphaga
Chlorhexidine
Antioxidant activity
Oxidative stress
url http://www.sciencedirect.com/science/article/pii/S2213716522001205
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