Interactions of free-living amoebae with the rice fungal pathogen, Rhizoctonia solani
Abstract Objective Rhizoctonia solani is a soil-borne fungal pathogen of many important crop plants. In rice, R. solani causes sheath blight disease, which results in devastating grain yield and quality losses. Few methods are available to control this pathogen and classic single gene resistance mec...
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| Format: | Article |
| Language: | English |
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BMC
2019-11-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-019-4802-2 |
| _version_ | 1811293352660828160 |
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| author | John J. Long Emily K. Luna Mary Jackson William Wheat Courtney E. Jahn Jan E. Leach |
| author_facet | John J. Long Emily K. Luna Mary Jackson William Wheat Courtney E. Jahn Jan E. Leach |
| author_sort | John J. Long |
| collection | DOAJ |
| description | Abstract Objective Rhizoctonia solani is a soil-borne fungal pathogen of many important crop plants. In rice, R. solani causes sheath blight disease, which results in devastating grain yield and quality losses. Few methods are available to control this pathogen and classic single gene resistance mechanisms in rice plants have not been identified. We hypothesize that alternate means of control are available in the environment including free-living amoebae. Amoebae are soil-, water- and air-borne microorganisms that are predominantly heterotrophic. Many amoeba species are mycophagous, and several harm their prey using mechanisms other than phagocytosis. Here, we used light and scanning electron microscopy to survey the interactions of R. solani with four amoeba species, with the goal of identifying amoebae species with potential for biocontrol. Results We observed a wide range of responses during interactions of R. solani with four different free-living amoebae. Two Acanthamoeba species encyst in co-cultures with R. solani at higher rates than medium without R. solani. Vermamoeba vermiformis (formerly Hartmanella vermiformis) attach to R. solani mycelium and are associated with mycelial shriveling and perforations of fungal cell walls, indicating an antagonistic interaction. No phenotypic changes were observed in co-cultures of Dictyostelium discoideum and R. solani. |
| first_indexed | 2024-04-13T05:00:03Z |
| format | Article |
| id | doaj.art-9d688175798d47a7a52ab78749236d92 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-04-13T05:00:03Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-9d688175798d47a7a52ab78749236d922022-12-22T03:01:22ZengBMCBMC Research Notes1756-05002019-11-011211710.1186/s13104-019-4802-2Interactions of free-living amoebae with the rice fungal pathogen, Rhizoctonia solaniJohn J. Long0Emily K. Luna1Mary Jackson2William Wheat3Courtney E. Jahn4Jan E. Leach5Department of Plant Pathology, The Ohio State UniversityDepartment of Bioagricultural Sciences and Pest Management, Colorado State UniversityMycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State UniversityMycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State UniversityDepartment of Bioagricultural Sciences and Pest Management, Colorado State UniversityDepartment of Bioagricultural Sciences and Pest Management, Colorado State UniversityAbstract Objective Rhizoctonia solani is a soil-borne fungal pathogen of many important crop plants. In rice, R. solani causes sheath blight disease, which results in devastating grain yield and quality losses. Few methods are available to control this pathogen and classic single gene resistance mechanisms in rice plants have not been identified. We hypothesize that alternate means of control are available in the environment including free-living amoebae. Amoebae are soil-, water- and air-borne microorganisms that are predominantly heterotrophic. Many amoeba species are mycophagous, and several harm their prey using mechanisms other than phagocytosis. Here, we used light and scanning electron microscopy to survey the interactions of R. solani with four amoeba species, with the goal of identifying amoebae species with potential for biocontrol. Results We observed a wide range of responses during interactions of R. solani with four different free-living amoebae. Two Acanthamoeba species encyst in co-cultures with R. solani at higher rates than medium without R. solani. Vermamoeba vermiformis (formerly Hartmanella vermiformis) attach to R. solani mycelium and are associated with mycelial shriveling and perforations of fungal cell walls, indicating an antagonistic interaction. No phenotypic changes were observed in co-cultures of Dictyostelium discoideum and R. solani.http://link.springer.com/article/10.1186/s13104-019-4802-2Free-living amoebaeRhizoctonia solaniSheath blightAcanthamoebaDictyosteliumVermamoeba |
| spellingShingle | John J. Long Emily K. Luna Mary Jackson William Wheat Courtney E. Jahn Jan E. Leach Interactions of free-living amoebae with the rice fungal pathogen, Rhizoctonia solani BMC Research Notes Free-living amoebae Rhizoctonia solani Sheath blight Acanthamoeba Dictyostelium Vermamoeba |
| title | Interactions of free-living amoebae with the rice fungal pathogen, Rhizoctonia solani |
| title_full | Interactions of free-living amoebae with the rice fungal pathogen, Rhizoctonia solani |
| title_fullStr | Interactions of free-living amoebae with the rice fungal pathogen, Rhizoctonia solani |
| title_full_unstemmed | Interactions of free-living amoebae with the rice fungal pathogen, Rhizoctonia solani |
| title_short | Interactions of free-living amoebae with the rice fungal pathogen, Rhizoctonia solani |
| title_sort | interactions of free living amoebae with the rice fungal pathogen rhizoctonia solani |
| topic | Free-living amoebae Rhizoctonia solani Sheath blight Acanthamoeba Dictyostelium Vermamoeba |
| url | http://link.springer.com/article/10.1186/s13104-019-4802-2 |
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