Association between Kidney Injury Molecule-1 Gene Polymorphism and Acute Kidney Injury among the Lebanese Patients
Acute kidney injury is a common condition associated with longer hospital stay and increased mortality. Kidney injury molecule-1 (KIM-1) is one of the early and sensitive biomarkers for acute kidney injury diagnosis. Therefore we examined the relationship between kidney injury molecule-1 gene polym...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Sarajevo, Institute for Genetic Engineering and Biotechnology
2019-12-01
|
| Series: | Genetics & Applications |
| Subjects: | |
| Online Access: | https://genapp.ba/editions/index.php/journal/article/view/58 |
| _version_ | 1828118696935030784 |
|---|---|
| author | Hajar Karaali Jamilah Borjac |
| author_facet | Hajar Karaali Jamilah Borjac |
| author_sort | Hajar Karaali |
| collection | DOAJ |
| description |
Acute kidney injury is a common condition associated with longer hospital stay and increased mortality. Kidney injury molecule-1 (KIM-1) is one of the early and sensitive biomarkers for acute kidney injury diagnosis. Therefore we examined the relationship between kidney injury molecule-1 gene polymorphism and acute kidney injury in Lebanese hospitalized patients. Genomic DNA was isolated from blood samples collected from 50 patients and 40 controls. Kidney injury molecule-1 exon 4 was amplified by polymerase chain reaction and the amplified products were sequenced at Macrogen. Serum creatinine and urea levels were measured and compared between controls and patients. Three out of the five known single nucleotide polymorphisms showed significant association with susceptibility to the disease (P ≤ 0.05). Data analysis implied that carriers of the risk allele of these 3 single nucleotide polymorphisms were more predisposed to acute kidney injury. No association was found between the studied nucleotides variations and creatinine/urea levels. Haplotype analysis showed high association of the block CTA with acute kidney injury incidence and high creatinine and urea levels. Our results suggest that polymorphisms in exon 4 of kidney injury molecule-1 in the Lebanese population may be associated with acute kidney injury.
|
| first_indexed | 2024-04-11T13:36:39Z |
| format | Article |
| id | doaj.art-9d6df1b4e3604ffa957ef69028c0ad71 |
| institution | Directory Open Access Journal |
| issn | 2566-2937 2566-431X |
| language | English |
| last_indexed | 2024-04-11T13:36:39Z |
| publishDate | 2019-12-01 |
| publisher | University of Sarajevo, Institute for Genetic Engineering and Biotechnology |
| record_format | Article |
| series | Genetics & Applications |
| spelling | doaj.art-9d6df1b4e3604ffa957ef69028c0ad712022-12-22T04:21:27ZengUniversity of Sarajevo, Institute for Genetic Engineering and BiotechnologyGenetics & Applications2566-29372566-431X2019-12-013310.31383/ga.vol3iss3pp9-16Association between Kidney Injury Molecule-1 Gene Polymorphism and Acute Kidney Injury among the Lebanese PatientsHajar Karaali0Jamilah Borjac1Department of Biological Sciences, Faculty of Science, Beirut Arab University, Debbieh, LebanonDepartment of Biological Sciences, Faculty of Science, Beirut Arab University, Debbieh, Lebanon Acute kidney injury is a common condition associated with longer hospital stay and increased mortality. Kidney injury molecule-1 (KIM-1) is one of the early and sensitive biomarkers for acute kidney injury diagnosis. Therefore we examined the relationship between kidney injury molecule-1 gene polymorphism and acute kidney injury in Lebanese hospitalized patients. Genomic DNA was isolated from blood samples collected from 50 patients and 40 controls. Kidney injury molecule-1 exon 4 was amplified by polymerase chain reaction and the amplified products were sequenced at Macrogen. Serum creatinine and urea levels were measured and compared between controls and patients. Three out of the five known single nucleotide polymorphisms showed significant association with susceptibility to the disease (P ≤ 0.05). Data analysis implied that carriers of the risk allele of these 3 single nucleotide polymorphisms were more predisposed to acute kidney injury. No association was found between the studied nucleotides variations and creatinine/urea levels. Haplotype analysis showed high association of the block CTA with acute kidney injury incidence and high creatinine and urea levels. Our results suggest that polymorphisms in exon 4 of kidney injury molecule-1 in the Lebanese population may be associated with acute kidney injury. https://genapp.ba/editions/index.php/journal/article/view/58Acute kidney injuryHaplotypeKidney Injury MoleculelPolymorphism |
| spellingShingle | Hajar Karaali Jamilah Borjac Association between Kidney Injury Molecule-1 Gene Polymorphism and Acute Kidney Injury among the Lebanese Patients Genetics & Applications Acute kidney injury Haplotype Kidney Injury Moleculel Polymorphism |
| title | Association between Kidney Injury Molecule-1 Gene Polymorphism and Acute Kidney Injury among the Lebanese Patients |
| title_full | Association between Kidney Injury Molecule-1 Gene Polymorphism and Acute Kidney Injury among the Lebanese Patients |
| title_fullStr | Association between Kidney Injury Molecule-1 Gene Polymorphism and Acute Kidney Injury among the Lebanese Patients |
| title_full_unstemmed | Association between Kidney Injury Molecule-1 Gene Polymorphism and Acute Kidney Injury among the Lebanese Patients |
| title_short | Association between Kidney Injury Molecule-1 Gene Polymorphism and Acute Kidney Injury among the Lebanese Patients |
| title_sort | association between kidney injury molecule 1 gene polymorphism and acute kidney injury among the lebanese patients |
| topic | Acute kidney injury Haplotype Kidney Injury Moleculel Polymorphism |
| url | https://genapp.ba/editions/index.php/journal/article/view/58 |
| work_keys_str_mv | AT hajarkaraali associationbetweenkidneyinjurymolecule1genepolymorphismandacutekidneyinjuryamongthelebanesepatients AT jamilahborjac associationbetweenkidneyinjurymolecule1genepolymorphismandacutekidneyinjuryamongthelebanesepatients |