RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters
Although high altitude training has been increasingly popular among endurance athletes, the molecular and cellular bases of this adaptation remain poorly understood. We aimed to define the underlying physiological changes and screen for potential biomarkers of adaptation using transcriptional profil...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/4/574 |
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| author | Andrey S. Glotov Irina E. Zelenkova Elena S. Vashukova Anna R. Shuvalova Alexandra D. Zolotareva Dmitrii E. Polev Yury A. Barbitoff Oleg S. Glotov Andrey M. Sarana Sergey G. Shcherbak Mariya A. Rozina Victoria L. Gogotova Alexander V. Predeus |
| author_facet | Andrey S. Glotov Irina E. Zelenkova Elena S. Vashukova Anna R. Shuvalova Alexandra D. Zolotareva Dmitrii E. Polev Yury A. Barbitoff Oleg S. Glotov Andrey M. Sarana Sergey G. Shcherbak Mariya A. Rozina Victoria L. Gogotova Alexander V. Predeus |
| author_sort | Andrey S. Glotov |
| collection | DOAJ |
| description | Although high altitude training has been increasingly popular among endurance athletes, the molecular and cellular bases of this adaptation remain poorly understood. We aimed to define the underlying physiological changes and screen for potential biomarkers of adaptation using transcriptional profiling of whole blood. Seven elite female speed skaters were profiled on the 18th day of high-altitude adaptation. Whole blood RNA-seq before and after an intense 1 h skating bout was used to measure gene expression changes associated with exercise. In order to identify the genes specifically regulated at high altitudes, we have leveraged the data from eight previously published microarray datasets studying blood expression changes after exercise at sea level. Using cell type-specific signatures, we were able to deconvolute changes of cell type abundance from individual gene expression changes. Among these were <i>PHOSPHO1</i>, with a known role in erythropoiesis, and <i>MARC1</i> with a role in endogenic NO metabolism. We find that platelet and erythrocyte counts uniquely respond to altitude exercise, while changes in neutrophils represent a more generic marker of intense exercise. Publicly available data from both single cell atlases and exercise-related blood profiling dramatically increases the value of whole blood RNA-seq for the dynamic evaluation of physiological changes in an athlete’s body. |
| first_indexed | 2024-03-09T10:36:26Z |
| format | Article |
| id | doaj.art-9d70caceb8534eb6a468dcc9a4edbcec |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T10:36:26Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-9d70caceb8534eb6a468dcc9a4edbcec2023-12-01T20:56:24ZengMDPI AGGenes2073-44252022-03-0113457410.3390/genes13040574RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed SkatersAndrey S. Glotov0Irina E. Zelenkova1Elena S. Vashukova2Anna R. Shuvalova3Alexandra D. Zolotareva4Dmitrii E. Polev5Yury A. Barbitoff6Oleg S. Glotov7Andrey M. Sarana8Sergey G. Shcherbak9Mariya A. Rozina10Victoria L. Gogotova11Alexander V. Predeus12Laboratory of Biobanking and Genome Medicine, Institute of Translational Biomedicine, Saint Petersburg State University, Universitetskaya nab., 7-9-11, 199034 St. Petersburg, RussiaGENUD Research Group, FIMS Collaborating Center of Sports Medicine, Department of Physiatry and Nursing, University of Zaragoza, 50009 Zaragoza, SpainDivision of Genome Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya 3, 199034 St. Petersburg, RussiaDivision of Genome Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya 3, 199034 St. Petersburg, RussiaDivision of Genome Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya 3, 199034 St. Petersburg, RussiaDivision of Genome Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya 3, 199034 St. Petersburg, RussiaDivision of Genome Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya 3, 199034 St. Petersburg, RussiaDivision of Genome Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, Mendeleevskaya 3, 199034 St. Petersburg, RussiaCity Hospital #40, Sestroretsk, Borisov Str., 9, 197706 St. Petersburg, RussiaCity Hospital #40, Sestroretsk, Borisov Str., 9, 197706 St. Petersburg, RussiaRussian Skating Union, Luzhnetskaya nab., 8, Office 230, 119991 Moscow, RussiaRussian Skating Union, Luzhnetskaya nab., 8, Office 230, 119991 Moscow, RussiaBioinformatics Institute, Kantemirovskaya St., 2A, 194100 St. Petersburg, RussiaAlthough high altitude training has been increasingly popular among endurance athletes, the molecular and cellular bases of this adaptation remain poorly understood. We aimed to define the underlying physiological changes and screen for potential biomarkers of adaptation using transcriptional profiling of whole blood. Seven elite female speed skaters were profiled on the 18th day of high-altitude adaptation. Whole blood RNA-seq before and after an intense 1 h skating bout was used to measure gene expression changes associated with exercise. In order to identify the genes specifically regulated at high altitudes, we have leveraged the data from eight previously published microarray datasets studying blood expression changes after exercise at sea level. Using cell type-specific signatures, we were able to deconvolute changes of cell type abundance from individual gene expression changes. Among these were <i>PHOSPHO1</i>, with a known role in erythropoiesis, and <i>MARC1</i> with a role in endogenic NO metabolism. We find that platelet and erythrocyte counts uniquely respond to altitude exercise, while changes in neutrophils represent a more generic marker of intense exercise. Publicly available data from both single cell atlases and exercise-related blood profiling dramatically increases the value of whole blood RNA-seq for the dynamic evaluation of physiological changes in an athlete’s body.https://www.mdpi.com/2073-4425/13/4/574RNA-seqRNA sequencingexerciseexpression profilingwhole bloodelite athletes |
| spellingShingle | Andrey S. Glotov Irina E. Zelenkova Elena S. Vashukova Anna R. Shuvalova Alexandra D. Zolotareva Dmitrii E. Polev Yury A. Barbitoff Oleg S. Glotov Andrey M. Sarana Sergey G. Shcherbak Mariya A. Rozina Victoria L. Gogotova Alexander V. Predeus RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters Genes RNA-seq RNA sequencing exercise expression profiling whole blood elite athletes |
| title | RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters |
| title_full | RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters |
| title_fullStr | RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters |
| title_full_unstemmed | RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters |
| title_short | RNA Sequencing of Whole Blood Defines the Signature of High Intensity Exercise at Altitude in Elite Speed Skaters |
| title_sort | rna sequencing of whole blood defines the signature of high intensity exercise at altitude in elite speed skaters |
| topic | RNA-seq RNA sequencing exercise expression profiling whole blood elite athletes |
| url | https://www.mdpi.com/2073-4425/13/4/574 |
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