Discovery pipeline for epigenetically deregulated miRNAs in cancer: integration of primary miRNA transcription
<p>Abstract</p> <p>Background</p> <p>Cancer is commonly associated with widespread disruption of DNA methylation, chromatin modification and miRNA expression. In this study, we established a robust discovery pipeline to identify epigenetically deregulated miRNAs in canc...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-01-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/12/54 |
| _version_ | 1811299495963525120 |
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| author | Statham Aaron L Strbenac Dario Bert Saul Wiklund Erik D Sibbritt Tennille Hulf Toby Robinson Mark D Clark Susan J |
| author_facet | Statham Aaron L Strbenac Dario Bert Saul Wiklund Erik D Sibbritt Tennille Hulf Toby Robinson Mark D Clark Susan J |
| author_sort | Statham Aaron L |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Cancer is commonly associated with widespread disruption of DNA methylation, chromatin modification and miRNA expression. In this study, we established a robust discovery pipeline to identify epigenetically deregulated miRNAs in cancer.</p> <p>Results</p> <p>Using an integrative approach that combines primary transcription, genome-wide DNA methylation and H3K9Ac marks with microRNA (miRNA) expression, we identified miRNA genes that were epigenetically modified in cancer. We find miR-205, miR-21, and miR-196b to be epigenetically repressed, and miR-615 epigenetically activated in prostate cancer cells.</p> <p>Conclusions</p> <p>We show that detecting changes in primary miRNA transcription levels is a valuable method for detection of local epigenetic modifications that are associated with changes in mature miRNA expression.</p> |
| first_indexed | 2024-04-13T06:35:46Z |
| format | Article |
| id | doaj.art-9d71836ca6c44063b12530d6fe23b1f8 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-13T06:35:46Z |
| publishDate | 2011-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-9d71836ca6c44063b12530d6fe23b1f82022-12-22T02:57:55ZengBMCBMC Genomics1471-21642011-01-011215410.1186/1471-2164-12-54Discovery pipeline for epigenetically deregulated miRNAs in cancer: integration of primary miRNA transcriptionStatham Aaron LStrbenac DarioBert SaulWiklund Erik DSibbritt TennilleHulf TobyRobinson Mark DClark Susan J<p>Abstract</p> <p>Background</p> <p>Cancer is commonly associated with widespread disruption of DNA methylation, chromatin modification and miRNA expression. In this study, we established a robust discovery pipeline to identify epigenetically deregulated miRNAs in cancer.</p> <p>Results</p> <p>Using an integrative approach that combines primary transcription, genome-wide DNA methylation and H3K9Ac marks with microRNA (miRNA) expression, we identified miRNA genes that were epigenetically modified in cancer. We find miR-205, miR-21, and miR-196b to be epigenetically repressed, and miR-615 epigenetically activated in prostate cancer cells.</p> <p>Conclusions</p> <p>We show that detecting changes in primary miRNA transcription levels is a valuable method for detection of local epigenetic modifications that are associated with changes in mature miRNA expression.</p>http://www.biomedcentral.com/1471-2164/12/54 |
| spellingShingle | Statham Aaron L Strbenac Dario Bert Saul Wiklund Erik D Sibbritt Tennille Hulf Toby Robinson Mark D Clark Susan J Discovery pipeline for epigenetically deregulated miRNAs in cancer: integration of primary miRNA transcription BMC Genomics |
| title | Discovery pipeline for epigenetically deregulated miRNAs in cancer: integration of primary miRNA transcription |
| title_full | Discovery pipeline for epigenetically deregulated miRNAs in cancer: integration of primary miRNA transcription |
| title_fullStr | Discovery pipeline for epigenetically deregulated miRNAs in cancer: integration of primary miRNA transcription |
| title_full_unstemmed | Discovery pipeline for epigenetically deregulated miRNAs in cancer: integration of primary miRNA transcription |
| title_short | Discovery pipeline for epigenetically deregulated miRNAs in cancer: integration of primary miRNA transcription |
| title_sort | discovery pipeline for epigenetically deregulated mirnas in cancer integration of primary mirna transcription |
| url | http://www.biomedcentral.com/1471-2164/12/54 |
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