Assessing MHC-B diversity in Silkie chickens
The major histocompatibility complex (MHC) is a highly polymorphic region on chromosome 16, which contains numerous immune response genes, and is known to influence disease susceptibility and resistance in chickens. Variability of MHC-B haplotypes in various well-known and commercially utilized bree...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-05-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579120300572 |
| _version_ | 1818348446520705024 |
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| author | Katy J. Tarrant Rodrigo Lopez Meghan Loper Janet E. Fulton |
| author_facet | Katy J. Tarrant Rodrigo Lopez Meghan Loper Janet E. Fulton |
| author_sort | Katy J. Tarrant |
| collection | DOAJ |
| description | The major histocompatibility complex (MHC) is a highly polymorphic region on chromosome 16, which contains numerous immune response genes, and is known to influence disease susceptibility and resistance in chickens. Variability of MHC-B haplotypes in various well-known and commercially utilized breeds has previously been identified. This study aims to understand MHC-B diversity in the Silkie breed using a high-density SNP panel that encompasses the chicken MHC-B region. DNA was obtained from 74 females and 27 males from a commercial Silkie breeder colony that is maintained through minimal genetic selection practices. A previously described panel of 90 SNPs, all located within the MHC-B region, was used to evaluate MHC-B variability in the commercial Silkie breeder colony. MHC-B haplotypes identified from the individual SNP information in the Silkie colony were compared to published haplotypes from the same region. Of the 27 haplotypes identified in the Silkie population, 8 have been previously described. Nineteen haplotypes are unique to the Silkie population and include one novel recombinant and 2 additional possible novel recombinants. Six haplotypes were found at a frequency greater than 5% of the population, of which 4 are novel. Finally, Hardy Weinberg Equilibrium (HWE) was calculated for the observed haplotypes, which were found to be in HWE. This study shows considerable MHC-B diversity in the Silkie breed and adds further information on variability of the MHC-B region in the chicken. |
| first_indexed | 2024-12-13T17:50:11Z |
| format | Article |
| id | doaj.art-9d72085c2e1f425099adf356e29028ae |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-13T17:50:11Z |
| publishDate | 2020-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-9d72085c2e1f425099adf356e29028ae2022-12-21T23:36:31ZengElsevierPoultry Science0032-57912020-05-0199523372341Assessing MHC-B diversity in Silkie chickensKaty J. Tarrant0Rodrigo Lopez1Meghan Loper2Janet E. Fulton3Department of Animal Sciences and Agricultural Education, California State University Fresno, Fresno 93740, USA; Corresponding author:Department of Animal Sciences and Agricultural Education, California State University Fresno, Fresno 93740, USAPitman Family Farms, Sanger, CA 93657, USAPitman Family Farms, Sanger, CA 93657, USA; Hy-Line International, Dallas Center, IA 50063, USAThe major histocompatibility complex (MHC) is a highly polymorphic region on chromosome 16, which contains numerous immune response genes, and is known to influence disease susceptibility and resistance in chickens. Variability of MHC-B haplotypes in various well-known and commercially utilized breeds has previously been identified. This study aims to understand MHC-B diversity in the Silkie breed using a high-density SNP panel that encompasses the chicken MHC-B region. DNA was obtained from 74 females and 27 males from a commercial Silkie breeder colony that is maintained through minimal genetic selection practices. A previously described panel of 90 SNPs, all located within the MHC-B region, was used to evaluate MHC-B variability in the commercial Silkie breeder colony. MHC-B haplotypes identified from the individual SNP information in the Silkie colony were compared to published haplotypes from the same region. Of the 27 haplotypes identified in the Silkie population, 8 have been previously described. Nineteen haplotypes are unique to the Silkie population and include one novel recombinant and 2 additional possible novel recombinants. Six haplotypes were found at a frequency greater than 5% of the population, of which 4 are novel. Finally, Hardy Weinberg Equilibrium (HWE) was calculated for the observed haplotypes, which were found to be in HWE. This study shows considerable MHC-B diversity in the Silkie breed and adds further information on variability of the MHC-B region in the chicken.http://www.sciencedirect.com/science/article/pii/S0032579120300572SilkieMHC-BhaplotypeSNP |
| spellingShingle | Katy J. Tarrant Rodrigo Lopez Meghan Loper Janet E. Fulton Assessing MHC-B diversity in Silkie chickens Poultry Science Silkie MHC-B haplotype SNP |
| title | Assessing MHC-B diversity in Silkie chickens |
| title_full | Assessing MHC-B diversity in Silkie chickens |
| title_fullStr | Assessing MHC-B diversity in Silkie chickens |
| title_full_unstemmed | Assessing MHC-B diversity in Silkie chickens |
| title_short | Assessing MHC-B diversity in Silkie chickens |
| title_sort | assessing mhc b diversity in silkie chickens |
| topic | Silkie MHC-B haplotype SNP |
| url | http://www.sciencedirect.com/science/article/pii/S0032579120300572 |
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