Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector
Bemisia tabaci (Hemiptera: Aleyrodidae) is a highly efficient vector in the spread of chilli leaf curl virus (ChiLCV, Begomovirus) which is a major constraint in the production of chilli in South Asia. Transcriptome analysis of B. tabaci post-6 h acquisition of ChiLCV showed differential expression...
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.890807/full |
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| author | Aarthi Nekkanti Aarthi Nekkanti Prosenjit Chakraborty Amalendu Ghosh Mir Asif Iquebal Sarika Jaiswal Virendra Kumar Baranwal |
| author_facet | Aarthi Nekkanti Aarthi Nekkanti Prosenjit Chakraborty Amalendu Ghosh Mir Asif Iquebal Sarika Jaiswal Virendra Kumar Baranwal |
| author_sort | Aarthi Nekkanti |
| collection | DOAJ |
| description | Bemisia tabaci (Hemiptera: Aleyrodidae) is a highly efficient vector in the spread of chilli leaf curl virus (ChiLCV, Begomovirus) which is a major constraint in the production of chilli in South Asia. Transcriptome analysis of B. tabaci post-6 h acquisition of ChiLCV showed differential expression of 80 (29 upregulated and 51 downregulated) genes. The maximum number of DEGs are categorized under the biological processes category followed by cellular components and molecular functions. KEGG analysis of DEGs showed that the genes are involved in the functions like metabolism, signaling pathways, cellular processes, and organismal systems. The expression of highly expressed 20 genes post-ChiLCV acquisition was validated in RT-qPCR. DEGs such as cytosolic carboxypeptidase 3, dual-specificity protein phosphatase 10, 15, dynein axonemal heavy chain 17, fasciclin 2, inhibin beta chain, replication factor A protein 1, and Tob1 were found enriched and favored the virus infection and circulation in B. tabaci. The present study provides an improved understanding of the networks of molecular interactions between B. tabaci and ChiLCV. The candidate genes of B. tabaci involved in ChiLCV transmission would be novel targets for the management of the B. tabaci-begomovirus complex. |
| first_indexed | 2024-12-10T09:26:36Z |
| format | Article |
| id | doaj.art-9d72f8179d704c2cb320cfcb34b0cf11 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T09:26:36Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9d72f8179d704c2cb320cfcb34b0cf112022-12-22T01:54:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-04-011310.3389/fmicb.2022.890807890807Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its VectorAarthi Nekkanti0Aarthi Nekkanti1Prosenjit Chakraborty2Amalendu Ghosh3Mir Asif Iquebal4Sarika Jaiswal5Virendra Kumar Baranwal6Advanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, IndiaDivision of Entomology, Indian Agricultural Research Institute, New Delhi, IndiaAdvanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, IndiaAdvanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, IndiaCentre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute, New Delhi, IndiaCentre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute, New Delhi, IndiaAdvanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, IndiaBemisia tabaci (Hemiptera: Aleyrodidae) is a highly efficient vector in the spread of chilli leaf curl virus (ChiLCV, Begomovirus) which is a major constraint in the production of chilli in South Asia. Transcriptome analysis of B. tabaci post-6 h acquisition of ChiLCV showed differential expression of 80 (29 upregulated and 51 downregulated) genes. The maximum number of DEGs are categorized under the biological processes category followed by cellular components and molecular functions. KEGG analysis of DEGs showed that the genes are involved in the functions like metabolism, signaling pathways, cellular processes, and organismal systems. The expression of highly expressed 20 genes post-ChiLCV acquisition was validated in RT-qPCR. DEGs such as cytosolic carboxypeptidase 3, dual-specificity protein phosphatase 10, 15, dynein axonemal heavy chain 17, fasciclin 2, inhibin beta chain, replication factor A protein 1, and Tob1 were found enriched and favored the virus infection and circulation in B. tabaci. The present study provides an improved understanding of the networks of molecular interactions between B. tabaci and ChiLCV. The candidate genes of B. tabaci involved in ChiLCV transmission would be novel targets for the management of the B. tabaci-begomovirus complex.https://www.frontiersin.org/articles/10.3389/fmicb.2022.890807/fullChiLCVsilverleaf whiteflyRNA-SeqtranscriptomeRT-qPCRvirus-vector relationship |
| spellingShingle | Aarthi Nekkanti Aarthi Nekkanti Prosenjit Chakraborty Amalendu Ghosh Mir Asif Iquebal Sarika Jaiswal Virendra Kumar Baranwal Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector Frontiers in Microbiology ChiLCV silverleaf whitefly RNA-Seq transcriptome RT-qPCR virus-vector relationship |
| title | Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector |
| title_full | Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector |
| title_fullStr | Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector |
| title_full_unstemmed | Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector |
| title_short | Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector |
| title_sort | transcriptomic changes of bemisia tabaci asia ii 1 induced by chilli leaf curl virus trigger infection and circulation in its vector |
| topic | ChiLCV silverleaf whitefly RNA-Seq transcriptome RT-qPCR virus-vector relationship |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.890807/full |
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