The Influence of Different Irradiation Regimens on Inflammation and Vascularization in a Random-Pattern Flap Model
Background: Irradiation plays an important role in the oncological treatment of various tumor entities. The aim of the study was to investigate the influence of different irradiation regimens on random-pattern flaps at the molecular and histopathological levels. Methods: Twenty-five rats underwent h...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
|
| Series: | Journal of Personalized Medicine |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4426/13/10/1514 |
| _version_ | 1797573314917433344 |
|---|---|
| author | Wibke Müller-Seubert Patrick Ostermaier Raymund E. Horch Luitpold Distel Benjamin Frey Ramona Erber Andreas Arkudas |
| author_facet | Wibke Müller-Seubert Patrick Ostermaier Raymund E. Horch Luitpold Distel Benjamin Frey Ramona Erber Andreas Arkudas |
| author_sort | Wibke Müller-Seubert |
| collection | DOAJ |
| description | Background: Irradiation plays an important role in the oncological treatment of various tumor entities. The aim of the study was to investigate the influence of different irradiation regimens on random-pattern flaps at the molecular and histopathological levels. Methods: Twenty-five rats underwent harvesting of bilateral random-pattern fasciocutaneous flaps. The right flaps received irradiation, while the left flaps served as non-irradiated intraindividual controls. Five rats served as a non-irradiated control group. Four different irradiation regimens with give rats each were tested: 20 Gy postoperatively, 3 × 12 Gy postoperatively, 20 Gy preoperatively, and 3 × 12 Gy preoperatively. Two weeks after surgery, HE staining and immunohistochemical staining for CD68 and ERG, as well as PCR analysis to detect Interleukin 6, HIF-1α, and VEGF, were performed. Results: A postoperative cumulative higher dose of irradiation appeared to result in an increase in necrosis, especially in the superficial layers of the flap compared to preoperative or single-stage irradiation. In addition, we observed increased expression of VEGF and HIF-1α in all irradiation groups. Conclusion: Even though no statistically significant differences were found between the different groups, there was a tendency for fractional postoperative irradiation with a higher total dose to have a more harmful effect compared to preoperative or single-dose irradiation. |
| first_indexed | 2024-03-10T21:08:05Z |
| format | Article |
| id | doaj.art-9d744b87e83b41a59dfdbc7f5da40902 |
| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-10T21:08:05Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Personalized Medicine |
| spelling | doaj.art-9d744b87e83b41a59dfdbc7f5da409022023-11-19T17:02:42ZengMDPI AGJournal of Personalized Medicine2075-44262023-10-011310151410.3390/jpm13101514The Influence of Different Irradiation Regimens on Inflammation and Vascularization in a Random-Pattern Flap ModelWibke Müller-Seubert0Patrick Ostermaier1Raymund E. Horch2Luitpold Distel3Benjamin Frey4Ramona Erber5Andreas Arkudas6Laboratory for Tissue Engineering and Regenerative Medicine, Department of Plastic and Hand Surgery, University Hospital Erlangen, Friedrich Alexander University Erlangen-Nuernberg (FAU), 91054 Erlangen, GermanyLaboratory for Tissue Engineering and Regenerative Medicine, Department of Plastic and Hand Surgery, University Hospital Erlangen, Friedrich Alexander University Erlangen-Nuernberg (FAU), 91054 Erlangen, GermanyLaboratory for Tissue Engineering and Regenerative Medicine, Department of Plastic and Hand Surgery, University Hospital Erlangen, Friedrich Alexander University Erlangen-Nuernberg (FAU), 91054 Erlangen, GermanyDepartment of Radiation Oncology, University Hospital Erlangen, Friedrich Alexander University Erlangen-Nuernberg (FAU), 91054 Erlangen, GermanyTranslational Radiobiology, Department of Radiation Oncology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nuernberg (FAU), 91054 Erlangen, GermanyInstitute of Pathology, University Hospital Erlangen, Friedrich Alexander University Erlangen-Nuernberg (FAU), Comprehensive Cancer Center Erlangen-EMN, 91054 Erlangen, GermanyLaboratory for Tissue Engineering and Regenerative Medicine, Department of Plastic and Hand Surgery, University Hospital Erlangen, Friedrich Alexander University Erlangen-Nuernberg (FAU), 91054 Erlangen, GermanyBackground: Irradiation plays an important role in the oncological treatment of various tumor entities. The aim of the study was to investigate the influence of different irradiation regimens on random-pattern flaps at the molecular and histopathological levels. Methods: Twenty-five rats underwent harvesting of bilateral random-pattern fasciocutaneous flaps. The right flaps received irradiation, while the left flaps served as non-irradiated intraindividual controls. Five rats served as a non-irradiated control group. Four different irradiation regimens with give rats each were tested: 20 Gy postoperatively, 3 × 12 Gy postoperatively, 20 Gy preoperatively, and 3 × 12 Gy preoperatively. Two weeks after surgery, HE staining and immunohistochemical staining for CD68 and ERG, as well as PCR analysis to detect Interleukin 6, HIF-1α, and VEGF, were performed. Results: A postoperative cumulative higher dose of irradiation appeared to result in an increase in necrosis, especially in the superficial layers of the flap compared to preoperative or single-stage irradiation. In addition, we observed increased expression of VEGF and HIF-1α in all irradiation groups. Conclusion: Even though no statistically significant differences were found between the different groups, there was a tendency for fractional postoperative irradiation with a higher total dose to have a more harmful effect compared to preoperative or single-dose irradiation.https://www.mdpi.com/2075-4426/13/10/1514irradiationrandom-pattern flapsvascularization |
| spellingShingle | Wibke Müller-Seubert Patrick Ostermaier Raymund E. Horch Luitpold Distel Benjamin Frey Ramona Erber Andreas Arkudas The Influence of Different Irradiation Regimens on Inflammation and Vascularization in a Random-Pattern Flap Model Journal of Personalized Medicine irradiation random-pattern flaps vascularization |
| title | The Influence of Different Irradiation Regimens on Inflammation and Vascularization in a Random-Pattern Flap Model |
| title_full | The Influence of Different Irradiation Regimens on Inflammation and Vascularization in a Random-Pattern Flap Model |
| title_fullStr | The Influence of Different Irradiation Regimens on Inflammation and Vascularization in a Random-Pattern Flap Model |
| title_full_unstemmed | The Influence of Different Irradiation Regimens on Inflammation and Vascularization in a Random-Pattern Flap Model |
| title_short | The Influence of Different Irradiation Regimens on Inflammation and Vascularization in a Random-Pattern Flap Model |
| title_sort | influence of different irradiation regimens on inflammation and vascularization in a random pattern flap model |
| topic | irradiation random-pattern flaps vascularization |
| url | https://www.mdpi.com/2075-4426/13/10/1514 |
| work_keys_str_mv | AT wibkemullerseubert theinfluenceofdifferentirradiationregimensoninflammationandvascularizationinarandompatternflapmodel AT patrickostermaier theinfluenceofdifferentirradiationregimensoninflammationandvascularizationinarandompatternflapmodel AT raymundehorch theinfluenceofdifferentirradiationregimensoninflammationandvascularizationinarandompatternflapmodel AT luitpolddistel theinfluenceofdifferentirradiationregimensoninflammationandvascularizationinarandompatternflapmodel AT benjaminfrey theinfluenceofdifferentirradiationregimensoninflammationandvascularizationinarandompatternflapmodel AT ramonaerber theinfluenceofdifferentirradiationregimensoninflammationandvascularizationinarandompatternflapmodel AT andreasarkudas theinfluenceofdifferentirradiationregimensoninflammationandvascularizationinarandompatternflapmodel AT wibkemullerseubert influenceofdifferentirradiationregimensoninflammationandvascularizationinarandompatternflapmodel AT patrickostermaier influenceofdifferentirradiationregimensoninflammationandvascularizationinarandompatternflapmodel AT raymundehorch influenceofdifferentirradiationregimensoninflammationandvascularizationinarandompatternflapmodel AT luitpolddistel influenceofdifferentirradiationregimensoninflammationandvascularizationinarandompatternflapmodel AT benjaminfrey influenceofdifferentirradiationregimensoninflammationandvascularizationinarandompatternflapmodel AT ramonaerber influenceofdifferentirradiationregimensoninflammationandvascularizationinarandompatternflapmodel AT andreasarkudas influenceofdifferentirradiationregimensoninflammationandvascularizationinarandompatternflapmodel |