DIA proteomics identified the potential targets associated with angiogenesis in the mammary glands of dairy cows with hemorrhagic mastitis
Hemorrhagic mastitis (HM) in dairy cows caused great economic losses in the dairy industry due to decreased milk production and increased costs associated with cattle management and treatment. However, the pathological and molecular mechanisms of HM are not well-understood. The present study aimed t...
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.980963/full |
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| author | Quanwei Zhang Quanwei Zhang Quanwei Zhang Xu Bai Xu Bai Jun Shi Jun Shi Xueying Wang Xueying Wang Bohao Zhang Bohao Zhang Lijun Dai Lijun Dai Ting Lin Ting Lin Yuan Gao Yuan Gao Yong Zhang Yong Zhang Yong Zhang Xingxu Zhao Xingxu Zhao Xingxu Zhao |
| author_facet | Quanwei Zhang Quanwei Zhang Quanwei Zhang Xu Bai Xu Bai Jun Shi Jun Shi Xueying Wang Xueying Wang Bohao Zhang Bohao Zhang Lijun Dai Lijun Dai Ting Lin Ting Lin Yuan Gao Yuan Gao Yong Zhang Yong Zhang Yong Zhang Xingxu Zhao Xingxu Zhao Xingxu Zhao |
| author_sort | Quanwei Zhang |
| collection | DOAJ |
| description | Hemorrhagic mastitis (HM) in dairy cows caused great economic losses in the dairy industry due to decreased milk production and increased costs associated with cattle management and treatment. However, the pathological and molecular mechanisms of HM are not well-understood. The present study aimed to investigate differentially expressed proteins (DEPs) associated with HM according to data-independent acquisition (DIA) proteomics. Compared to the mammary glands of healthylactating Holstein cows (Control, C group), the pathology of the HM group displayed massive alveolar infiltration of hemocytes and neutrophils, and the blood vessels, including arteriole, venules and capillaries were incomplete and damaged, with a loss of endothelial cells. DIA proteomics results showed that a total of 3,739 DEPs and 819 biological process terms were screened in the HM group. We focused on the blood, permeability of blood vessel, vascular and angiogenesis of mammary glands, and a total of 99 candidate DEPs, including 60 up- and 39 down-regulated DEPs, were obtained from the Gene Ontology (GO) and Pathway enrichment analyses. Phenotype prediction and function analysis of the DEPs revealed that three DEPs, particularly Caveolin-1(CAV1), were participated in the regulation of angiogenesis. Immunohistochemical and immunofluorescence staining showed that the CAV1 protein was present mainly in the mammary epithelial cells, vascular endothelial cells and vascular smooth muscle cells. The expression level of CAV1 mRNA and protein in the HM group was significantly down-regulated. The results will be helpful to the further understanding of the pathological and molecular mechanisms of HM in dairy cows. |
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| issn | 2297-1769 |
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| last_indexed | 2024-04-13T11:14:33Z |
| publishDate | 2022-08-01 |
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| spelling | doaj.art-9d78b3da87b04eaf8e81953fdb7a331c2022-12-22T02:49:02ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-08-01910.3389/fvets.2022.980963980963DIA proteomics identified the potential targets associated with angiogenesis in the mammary glands of dairy cows with hemorrhagic mastitisQuanwei Zhang0Quanwei Zhang1Quanwei Zhang2Xu Bai3Xu Bai4Jun Shi5Jun Shi6Xueying Wang7Xueying Wang8Bohao Zhang9Bohao Zhang10Lijun Dai11Lijun Dai12Ting Lin13Ting Lin14Yuan Gao15Yuan Gao16Yong Zhang17Yong Zhang18Yong Zhang19Xingxu Zhao20Xingxu Zhao21Xingxu Zhao22College of Veterinary Medicine, Gansu Agriculture University, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agriculture University, Lanzhou, ChinaGansu Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agriculture University, Lanzhou, ChinaGansu Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agriculture University, Lanzhou, ChinaGansu Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agriculture University, Lanzhou, ChinaGansu Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agriculture University, Lanzhou, ChinaGansu Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agriculture University, Lanzhou, ChinaGansu Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agriculture University, Lanzhou, ChinaGansu Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agriculture University, Lanzhou, ChinaGansu Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agriculture University, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agriculture University, Lanzhou, ChinaGansu Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agriculture University, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agriculture University, Lanzhou, ChinaGansu Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation, Lanzhou, ChinaHemorrhagic mastitis (HM) in dairy cows caused great economic losses in the dairy industry due to decreased milk production and increased costs associated with cattle management and treatment. However, the pathological and molecular mechanisms of HM are not well-understood. The present study aimed to investigate differentially expressed proteins (DEPs) associated with HM according to data-independent acquisition (DIA) proteomics. Compared to the mammary glands of healthylactating Holstein cows (Control, C group), the pathology of the HM group displayed massive alveolar infiltration of hemocytes and neutrophils, and the blood vessels, including arteriole, venules and capillaries were incomplete and damaged, with a loss of endothelial cells. DIA proteomics results showed that a total of 3,739 DEPs and 819 biological process terms were screened in the HM group. We focused on the blood, permeability of blood vessel, vascular and angiogenesis of mammary glands, and a total of 99 candidate DEPs, including 60 up- and 39 down-regulated DEPs, were obtained from the Gene Ontology (GO) and Pathway enrichment analyses. Phenotype prediction and function analysis of the DEPs revealed that three DEPs, particularly Caveolin-1(CAV1), were participated in the regulation of angiogenesis. Immunohistochemical and immunofluorescence staining showed that the CAV1 protein was present mainly in the mammary epithelial cells, vascular endothelial cells and vascular smooth muscle cells. The expression level of CAV1 mRNA and protein in the HM group was significantly down-regulated. The results will be helpful to the further understanding of the pathological and molecular mechanisms of HM in dairy cows.https://www.frontiersin.org/articles/10.3389/fvets.2022.980963/fullangiogenesisCAV1hemorrhagic mastitisblood and cowDIA proteomics |
| spellingShingle | Quanwei Zhang Quanwei Zhang Quanwei Zhang Xu Bai Xu Bai Jun Shi Jun Shi Xueying Wang Xueying Wang Bohao Zhang Bohao Zhang Lijun Dai Lijun Dai Ting Lin Ting Lin Yuan Gao Yuan Gao Yong Zhang Yong Zhang Yong Zhang Xingxu Zhao Xingxu Zhao Xingxu Zhao DIA proteomics identified the potential targets associated with angiogenesis in the mammary glands of dairy cows with hemorrhagic mastitis Frontiers in Veterinary Science angiogenesis CAV1 hemorrhagic mastitis blood and cow DIA proteomics |
| title | DIA proteomics identified the potential targets associated with angiogenesis in the mammary glands of dairy cows with hemorrhagic mastitis |
| title_full | DIA proteomics identified the potential targets associated with angiogenesis in the mammary glands of dairy cows with hemorrhagic mastitis |
| title_fullStr | DIA proteomics identified the potential targets associated with angiogenesis in the mammary glands of dairy cows with hemorrhagic mastitis |
| title_full_unstemmed | DIA proteomics identified the potential targets associated with angiogenesis in the mammary glands of dairy cows with hemorrhagic mastitis |
| title_short | DIA proteomics identified the potential targets associated with angiogenesis in the mammary glands of dairy cows with hemorrhagic mastitis |
| title_sort | dia proteomics identified the potential targets associated with angiogenesis in the mammary glands of dairy cows with hemorrhagic mastitis |
| topic | angiogenesis CAV1 hemorrhagic mastitis blood and cow DIA proteomics |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.980963/full |
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