MiR-199a-3p affects the multi-chemoresistance of osteosarcoma through targeting AK4
Abstract Background MicroRNAs (miRNAs) play vital roles in regulating various biological processes. The dysregulations of miRNAs may result in severe human diseases, including cancer. Methods We performed the qRT-PCR, western blot and the luciferase reporter assays to test whether Adenylate Kinase 4...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12885-018-4460-0 |
| _version_ | 1818199647942868992 |
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| author | Wang Lei Chen Yan Jiang Ya Dai Yong Bian Yujun Liu Kai |
| author_facet | Wang Lei Chen Yan Jiang Ya Dai Yong Bian Yujun Liu Kai |
| author_sort | Wang Lei |
| collection | DOAJ |
| description | Abstract Background MicroRNAs (miRNAs) play vital roles in regulating various biological processes. The dysregulations of miRNAs may result in severe human diseases, including cancer. Methods We performed the qRT-PCR, western blot and the luciferase reporter assays to test whether Adenylate Kinase 4 (AK4) is the target of miR-199a-3p. Up- or down-regulation of miR-199a-3p and/or the AK4 gene was done to detect their roles in OS multi-drug resistance using drug resistance profiling assays. We further predicted the putative signal pathway involved in the miR-199a-3p-mediated OS drug-resistance. Results The AK4 gene is one of the targets of miR-199a-3p and negatively correlates with the effect of miR-199a-3p on OS drug-resistance. In addition, the activity of the NF-кB signaling pathway was drastically altered by the forced changes of the miR-199a-3p level in OS cells. Conclusions Our data revealed that both miR-199a-3p and its target gene AK4 are reversely correlated with the OS drug resistance. |
| first_indexed | 2024-12-12T02:25:06Z |
| format | Article |
| id | doaj.art-9d79001d16294b239e78220b6223513e |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-12T02:25:06Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-9d79001d16294b239e78220b6223513e2022-12-22T00:41:35ZengBMCBMC Cancer1471-24072018-06-011811910.1186/s12885-018-4460-0MiR-199a-3p affects the multi-chemoresistance of osteosarcoma through targeting AK4Wang Lei0Chen Yan1Jiang Ya2Dai Yong3Bian Yujun4Liu Kai5Department of orthopaedic surgery, the third people’s hospital of HefeiDepartment of orthopaedic surgery, the third people’s hospital of HefeiDepartment of orthopaedic surgery, the third people’s hospital of HefeiDepartment of orthopaedic surgery, the third people’s hospital of HefeiDepartment of orthopaedic surgery, the third people’s hospital of HefeiDepartment of orthopaedic surgery, the third people’s hospital of HefeiAbstract Background MicroRNAs (miRNAs) play vital roles in regulating various biological processes. The dysregulations of miRNAs may result in severe human diseases, including cancer. Methods We performed the qRT-PCR, western blot and the luciferase reporter assays to test whether Adenylate Kinase 4 (AK4) is the target of miR-199a-3p. Up- or down-regulation of miR-199a-3p and/or the AK4 gene was done to detect their roles in OS multi-drug resistance using drug resistance profiling assays. We further predicted the putative signal pathway involved in the miR-199a-3p-mediated OS drug-resistance. Results The AK4 gene is one of the targets of miR-199a-3p and negatively correlates with the effect of miR-199a-3p on OS drug-resistance. In addition, the activity of the NF-кB signaling pathway was drastically altered by the forced changes of the miR-199a-3p level in OS cells. Conclusions Our data revealed that both miR-199a-3p and its target gene AK4 are reversely correlated with the OS drug resistance.http://link.springer.com/article/10.1186/s12885-018-4460-0OsteosarcomaMulti-chemoresistancemiR-199a-3pAK4 |
| spellingShingle | Wang Lei Chen Yan Jiang Ya Dai Yong Bian Yujun Liu Kai MiR-199a-3p affects the multi-chemoresistance of osteosarcoma through targeting AK4 BMC Cancer Osteosarcoma Multi-chemoresistance miR-199a-3p AK4 |
| title | MiR-199a-3p affects the multi-chemoresistance of osteosarcoma through targeting AK4 |
| title_full | MiR-199a-3p affects the multi-chemoresistance of osteosarcoma through targeting AK4 |
| title_fullStr | MiR-199a-3p affects the multi-chemoresistance of osteosarcoma through targeting AK4 |
| title_full_unstemmed | MiR-199a-3p affects the multi-chemoresistance of osteosarcoma through targeting AK4 |
| title_short | MiR-199a-3p affects the multi-chemoresistance of osteosarcoma through targeting AK4 |
| title_sort | mir 199a 3p affects the multi chemoresistance of osteosarcoma through targeting ak4 |
| topic | Osteosarcoma Multi-chemoresistance miR-199a-3p AK4 |
| url | http://link.springer.com/article/10.1186/s12885-018-4460-0 |
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