Summary: | <i>Amorphophallus</i> is widely distributed in Southeast Asia, Africa, and other places, with more than 170 species. <i>Amorphophallus</i> has high medicinal value and is commonly used in medicine. However, the current classification based on morphology is challenging in with regard to <i>Amorphophallus</i> and closely related species. This study used six barcodes, namely ITS2, <i>matK</i>, <i>rcbL</i>, <i>nad1</i>, <i>trnH-psbA,</i> and <i>trnL-trnF</i>, to evaluate their identification ability for three important <i>Amorphophallus</i> species, including <i>A. konjac</i>, <i>A. albus</i>, and <i>A. muelleri</i>. We recommend that <i>trnH-psbA</i> can be applied to the <i>Amorphophallus</i> trade, quickly identify the purity of <i>A. konjac</i> and <i>A. albus</i> and distinguish <i>A. muelleri</i> from its related species for <i>A. konjac</i> and <i>A. albus</i> genetic improvement.
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