Screening of the Candidate DNA Barcodes for Three Important <i>Amorphophallus</i> Species Identification
<i>Amorphophallus</i> is widely distributed in Southeast Asia, Africa, and other places, with more than 170 species. <i>Amorphophallus</i> has high medicinal value and is commonly used in medicine. However, the current classification based on morphology is challenging in with...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/9/1366 |
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| author | Chufeng Zhao Xuan She Erxi Liu Nunung Harijati Teng Cheng Zhongli Hu Surong Jin Ying Diao |
| author_facet | Chufeng Zhao Xuan She Erxi Liu Nunung Harijati Teng Cheng Zhongli Hu Surong Jin Ying Diao |
| author_sort | Chufeng Zhao |
| collection | DOAJ |
| description | <i>Amorphophallus</i> is widely distributed in Southeast Asia, Africa, and other places, with more than 170 species. <i>Amorphophallus</i> has high medicinal value and is commonly used in medicine. However, the current classification based on morphology is challenging in with regard to <i>Amorphophallus</i> and closely related species. This study used six barcodes, namely ITS2, <i>matK</i>, <i>rcbL</i>, <i>nad1</i>, <i>trnH-psbA,</i> and <i>trnL-trnF</i>, to evaluate their identification ability for three important <i>Amorphophallus</i> species, including <i>A. konjac</i>, <i>A. albus</i>, and <i>A. muelleri</i>. We recommend that <i>trnH-psbA</i> can be applied to the <i>Amorphophallus</i> trade, quickly identify the purity of <i>A. konjac</i> and <i>A. albus</i> and distinguish <i>A. muelleri</i> from its related species for <i>A. konjac</i> and <i>A. albus</i> genetic improvement. |
| first_indexed | 2024-03-10T16:24:38Z |
| format | Article |
| id | doaj.art-9d79ea8cb589484ea3adf22ef5501c2c |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T16:24:38Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-9d79ea8cb589484ea3adf22ef5501c2c2023-11-20T13:19:58ZengMDPI AGAgronomy2073-43952020-09-01109136610.3390/agronomy10091366Screening of the Candidate DNA Barcodes for Three Important <i>Amorphophallus</i> Species IdentificationChufeng Zhao0Xuan She1Erxi Liu2Nunung Harijati3Teng Cheng4Zhongli Hu5Surong Jin6Ying Diao7State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl.Veteran Malang 65145, IndonesiaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaSchool of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China<i>Amorphophallus</i> is widely distributed in Southeast Asia, Africa, and other places, with more than 170 species. <i>Amorphophallus</i> has high medicinal value and is commonly used in medicine. However, the current classification based on morphology is challenging in with regard to <i>Amorphophallus</i> and closely related species. This study used six barcodes, namely ITS2, <i>matK</i>, <i>rcbL</i>, <i>nad1</i>, <i>trnH-psbA,</i> and <i>trnL-trnF</i>, to evaluate their identification ability for three important <i>Amorphophallus</i> species, including <i>A. konjac</i>, <i>A. albus</i>, and <i>A. muelleri</i>. We recommend that <i>trnH-psbA</i> can be applied to the <i>Amorphophallus</i> trade, quickly identify the purity of <i>A. konjac</i> and <i>A. albus</i> and distinguish <i>A. muelleri</i> from its related species for <i>A. konjac</i> and <i>A. albus</i> genetic improvement.https://www.mdpi.com/2073-4395/10/9/1366<i>Amorphophallus</i>authenticationDNA barcodes<i>trnH-psbA</i> |
| spellingShingle | Chufeng Zhao Xuan She Erxi Liu Nunung Harijati Teng Cheng Zhongli Hu Surong Jin Ying Diao Screening of the Candidate DNA Barcodes for Three Important <i>Amorphophallus</i> Species Identification Agronomy <i>Amorphophallus</i> authentication DNA barcodes <i>trnH-psbA</i> |
| title | Screening of the Candidate DNA Barcodes for Three Important <i>Amorphophallus</i> Species Identification |
| title_full | Screening of the Candidate DNA Barcodes for Three Important <i>Amorphophallus</i> Species Identification |
| title_fullStr | Screening of the Candidate DNA Barcodes for Three Important <i>Amorphophallus</i> Species Identification |
| title_full_unstemmed | Screening of the Candidate DNA Barcodes for Three Important <i>Amorphophallus</i> Species Identification |
| title_short | Screening of the Candidate DNA Barcodes for Three Important <i>Amorphophallus</i> Species Identification |
| title_sort | screening of the candidate dna barcodes for three important i amorphophallus i species identification |
| topic | <i>Amorphophallus</i> authentication DNA barcodes <i>trnH-psbA</i> |
| url | https://www.mdpi.com/2073-4395/10/9/1366 |
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