Evaluation of gene expression of transcription factors involved in salinity tolerance in Barley (Hordeum vulgare L.) advanced genotypes
ObjectiveSalinity stress is considered as one of the most important abiotic stresses that significantly decreased crop production in the world. The development of salt-tolerant varieties is one of the most strategies against decreasing crop performance in saline conditions. Hence, assessment of expr...
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Shahid Bahonar University of Kerman
2022-04-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
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| Online Access: | https://jab.uk.ac.ir/article_3214_76bfe9ae0b0983df4c217226166a9228.pdf |
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| author | Soraya Ghorbani Alireza Etminan Varahram Rashidi Alireza Pour-Aboughadareh Lia Shooshtari |
| author_facet | Soraya Ghorbani Alireza Etminan Varahram Rashidi Alireza Pour-Aboughadareh Lia Shooshtari |
| author_sort | Soraya Ghorbani |
| collection | DOAJ |
| description | ObjectiveSalinity stress is considered as one of the most important abiotic stresses that significantly decreased crop production in the world. The development of salt-tolerant varieties is one of the most strategies against decreasing crop performance in saline conditions. Hence, assessment of expression patterns of genes responsible for the biosynthesis pathways can be very effective in creating tolerant genotypes. The main objective of this study was to compression of expression patterns of HvSOS1, HvSOS3, HvNHX3, and HvHKT3 genes in some advanced barley genotypes under salinity stress conditions. Materials and methodsIn the present study, the effect of salt stress on relative changes expression of HvSOS1, HvSOS3, HvNHX3, and HvHKT3 genes in the six advanced barley genotypes along with Mehr cultivar (as a check) was investigated under the control (no NaCl) and salt (200 mM NaCl) treatments. All tested genotypes were planted under controlled greenhouse conditions using a hydroponic system. After salt treatment duration, all seedling plants were subjected to sampling and the relative expression of selected genes was estimated.ResultsBased on obtained results, there are significant differences between treatments as well as among tested genotypes. Salt treatment significantly increased expression of HvSOS1, HvSOS3, HvNHX3, and HvHKT3 genes by 14.76-, 4.65-, 4-, and 4-fold compared to the control treatment, respectively. Comparison of the expression patterns of studied genes showed that two genotypes G3 and G6 had the highest transcription rate of mentioned genes and it seems that they have an acceptable tolerance to the high levels of salinity stress. A positive association between HvHKT3 and HvSOS genes was another obtained result so that two identified genotypes indicated the highest expression of these genes under salt treatment. ConclusionsIt is inferred that increasing expression of studied genes is an important part of the salt tolerance mechanism because each of them has a key role in the signaling pathway and translocation of Na ions in the plant cells. Two identified genotypes, G3 and G6, with having this potential can be appreciated candidates for future research and use as salt-tolerant cultivars. |
| first_indexed | 2024-03-08T22:49:50Z |
| format | Article |
| id | doaj.art-9d7d32f477554e3ba81a8111254f3905 |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-03-08T22:49:50Z |
| publishDate | 2022-04-01 |
| publisher | Shahid Bahonar University of Kerman |
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| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-9d7d32f477554e3ba81a8111254f39052023-12-16T17:41:42ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002022-04-01141678410.22103/jab.2022.18546.13553214Evaluation of gene expression of transcription factors involved in salinity tolerance in Barley (Hordeum vulgare L.) advanced genotypesSoraya Ghorbani0Alireza Etminan1Varahram Rashidi2Alireza Pour-Aboughadareh3Lia Shooshtari4Department of Agronomy and plant breeding, Tabriz Branch, Islamic Azad University, Tabriz, Iran.Department of Plant breeding and Biotechnology, Kermanshah Branch, Islamic Azad University, Kermanshah, IranDepartment of Agronomy and plant breeding, Tabriz Branch, Islamic Azad University, Tabriz, Iran.Cereal Research, Seed and Plant Improvement Institute, Karaj, IranDepartment of Plant Breeding, Kermanshah Brunch, Islamic Azad University, Kermanshah, IranObjectiveSalinity stress is considered as one of the most important abiotic stresses that significantly decreased crop production in the world. The development of salt-tolerant varieties is one of the most strategies against decreasing crop performance in saline conditions. Hence, assessment of expression patterns of genes responsible for the biosynthesis pathways can be very effective in creating tolerant genotypes. The main objective of this study was to compression of expression patterns of HvSOS1, HvSOS3, HvNHX3, and HvHKT3 genes in some advanced barley genotypes under salinity stress conditions. Materials and methodsIn the present study, the effect of salt stress on relative changes expression of HvSOS1, HvSOS3, HvNHX3, and HvHKT3 genes in the six advanced barley genotypes along with Mehr cultivar (as a check) was investigated under the control (no NaCl) and salt (200 mM NaCl) treatments. All tested genotypes were planted under controlled greenhouse conditions using a hydroponic system. After salt treatment duration, all seedling plants were subjected to sampling and the relative expression of selected genes was estimated.ResultsBased on obtained results, there are significant differences between treatments as well as among tested genotypes. Salt treatment significantly increased expression of HvSOS1, HvSOS3, HvNHX3, and HvHKT3 genes by 14.76-, 4.65-, 4-, and 4-fold compared to the control treatment, respectively. Comparison of the expression patterns of studied genes showed that two genotypes G3 and G6 had the highest transcription rate of mentioned genes and it seems that they have an acceptable tolerance to the high levels of salinity stress. A positive association between HvHKT3 and HvSOS genes was another obtained result so that two identified genotypes indicated the highest expression of these genes under salt treatment. ConclusionsIt is inferred that increasing expression of studied genes is an important part of the salt tolerance mechanism because each of them has a key role in the signaling pathway and translocation of Na ions in the plant cells. Two identified genotypes, G3 and G6, with having this potential can be appreciated candidates for future research and use as salt-tolerant cultivars.https://jab.uk.ac.ir/article_3214_76bfe9ae0b0983df4c217226166a9228.pdfhydroponic systemtranscriptometranscription factorstolerant genes |
| spellingShingle | Soraya Ghorbani Alireza Etminan Varahram Rashidi Alireza Pour-Aboughadareh Lia Shooshtari Evaluation of gene expression of transcription factors involved in salinity tolerance in Barley (Hordeum vulgare L.) advanced genotypes مجله بیوتکنولوژی کشاورزی hydroponic system transcriptome transcription factors tolerant genes |
| title | Evaluation of gene expression of transcription factors involved in salinity tolerance in Barley (Hordeum vulgare L.) advanced genotypes |
| title_full | Evaluation of gene expression of transcription factors involved in salinity tolerance in Barley (Hordeum vulgare L.) advanced genotypes |
| title_fullStr | Evaluation of gene expression of transcription factors involved in salinity tolerance in Barley (Hordeum vulgare L.) advanced genotypes |
| title_full_unstemmed | Evaluation of gene expression of transcription factors involved in salinity tolerance in Barley (Hordeum vulgare L.) advanced genotypes |
| title_short | Evaluation of gene expression of transcription factors involved in salinity tolerance in Barley (Hordeum vulgare L.) advanced genotypes |
| title_sort | evaluation of gene expression of transcription factors involved in salinity tolerance in barley hordeum vulgare l advanced genotypes |
| topic | hydroponic system transcriptome transcription factors tolerant genes |
| url | https://jab.uk.ac.ir/article_3214_76bfe9ae0b0983df4c217226166a9228.pdf |
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