Axonal Guidance Using Biofunctionalized Straining Flow Spinning Regenerated Silk Fibroin Fibers as Scaffold
After an injury, the limited regenerative capacity of the central nervous system makes the reconnection and functional recovery of the affected nervous tissue almost impossible. To address this problem, biomaterials appear as a promising option for the design of scaffolds that promote and guide this...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Biomimetics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2313-7673/8/1/65 |
| _version_ | 1797613287614971904 |
|---|---|
| author | Cristina Castro-Domínguez Paloma Lozano-Picazo Aroa Álvarez-López Javier Garrote-Junco Fivos Panetsos Gustavo V. Guinea Manuel Elices Francisco Javier Rojo Daniel González-Nieto Luis Colchero Milagros Ramos José Pérez-Rigueiro |
| author_facet | Cristina Castro-Domínguez Paloma Lozano-Picazo Aroa Álvarez-López Javier Garrote-Junco Fivos Panetsos Gustavo V. Guinea Manuel Elices Francisco Javier Rojo Daniel González-Nieto Luis Colchero Milagros Ramos José Pérez-Rigueiro |
| author_sort | Cristina Castro-Domínguez |
| collection | DOAJ |
| description | After an injury, the limited regenerative capacity of the central nervous system makes the reconnection and functional recovery of the affected nervous tissue almost impossible. To address this problem, biomaterials appear as a promising option for the design of scaffolds that promote and guide this regenerative process. Based on previous seminal works on the ability of regenerated silk fibroin fibers spun through the straining flow spinning (SFS) technique, this study is intended to show that the usage of functionalized SFS fibers allows an enhancement of the guidance ability of the material when compared with the control (nonfunctionalized) fibers. It is shown that the axons of the neurons not only tend to follow the path marked by the fibers, in contrast to the isotropic growth observed on conventional culture plates, but also that this guidance can be further modulated through the biofunctionalization of the material with adhesion peptides. Establishing the guidance ability of these fibers opens the possibility of their use as implants for spinal cord injuries, so that they may represent the core of a therapy that would allow the reconnection of the injured ends of the spinal cord. |
| first_indexed | 2024-03-11T06:52:46Z |
| format | Article |
| id | doaj.art-9d7f0b7f34e74c59ab48ae3df58e0c40 |
| institution | Directory Open Access Journal |
| issn | 2313-7673 |
| language | English |
| last_indexed | 2024-03-11T06:52:46Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomimetics |
| spelling | doaj.art-9d7f0b7f34e74c59ab48ae3df58e0c402023-11-17T09:49:47ZengMDPI AGBiomimetics2313-76732023-02-01816510.3390/biomimetics8010065Axonal Guidance Using Biofunctionalized Straining Flow Spinning Regenerated Silk Fibroin Fibers as ScaffoldCristina Castro-Domínguez0Paloma Lozano-Picazo1Aroa Álvarez-López2Javier Garrote-Junco3Fivos Panetsos4Gustavo V. Guinea5Manuel Elices6Francisco Javier Rojo7Daniel González-Nieto8Luis Colchero9Milagros Ramos10José Pérez-Rigueiro11Neurocomputing and Neurorobotics Research Group, Faculty of Biology and Faculty of Optics, Universidad Complutense de Madrid, 28040 Madrid, SpainCenter for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223 Madrid, SpainCenter for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223 Madrid, SpainCenter for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223 Madrid, SpainNeurocomputing and Neurorobotics Research Group, Faculty of Biology and Faculty of Optics, Universidad Complutense de Madrid, 28040 Madrid, SpainCenter for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223 Madrid, SpainCenter for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223 Madrid, SpainCenter for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223 Madrid, SpainCenter for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223 Madrid, SpainCenter for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223 Madrid, SpainCenter for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223 Madrid, SpainCenter for Biomedical Technology, Universidad Politécnica de Madrid, Pozuelo de Alarcón, 28223 Madrid, SpainAfter an injury, the limited regenerative capacity of the central nervous system makes the reconnection and functional recovery of the affected nervous tissue almost impossible. To address this problem, biomaterials appear as a promising option for the design of scaffolds that promote and guide this regenerative process. Based on previous seminal works on the ability of regenerated silk fibroin fibers spun through the straining flow spinning (SFS) technique, this study is intended to show that the usage of functionalized SFS fibers allows an enhancement of the guidance ability of the material when compared with the control (nonfunctionalized) fibers. It is shown that the axons of the neurons not only tend to follow the path marked by the fibers, in contrast to the isotropic growth observed on conventional culture plates, but also that this guidance can be further modulated through the biofunctionalization of the material with adhesion peptides. Establishing the guidance ability of these fibers opens the possibility of their use as implants for spinal cord injuries, so that they may represent the core of a therapy that would allow the reconnection of the injured ends of the spinal cord.https://www.mdpi.com/2313-7673/8/1/65spinal cord injurysilk fibroinsilk biomaterialsstraining flow spinningaxonal guidanceregeneration |
| spellingShingle | Cristina Castro-Domínguez Paloma Lozano-Picazo Aroa Álvarez-López Javier Garrote-Junco Fivos Panetsos Gustavo V. Guinea Manuel Elices Francisco Javier Rojo Daniel González-Nieto Luis Colchero Milagros Ramos José Pérez-Rigueiro Axonal Guidance Using Biofunctionalized Straining Flow Spinning Regenerated Silk Fibroin Fibers as Scaffold Biomimetics spinal cord injury silk fibroin silk biomaterials straining flow spinning axonal guidance regeneration |
| title | Axonal Guidance Using Biofunctionalized Straining Flow Spinning Regenerated Silk Fibroin Fibers as Scaffold |
| title_full | Axonal Guidance Using Biofunctionalized Straining Flow Spinning Regenerated Silk Fibroin Fibers as Scaffold |
| title_fullStr | Axonal Guidance Using Biofunctionalized Straining Flow Spinning Regenerated Silk Fibroin Fibers as Scaffold |
| title_full_unstemmed | Axonal Guidance Using Biofunctionalized Straining Flow Spinning Regenerated Silk Fibroin Fibers as Scaffold |
| title_short | Axonal Guidance Using Biofunctionalized Straining Flow Spinning Regenerated Silk Fibroin Fibers as Scaffold |
| title_sort | axonal guidance using biofunctionalized straining flow spinning regenerated silk fibroin fibers as scaffold |
| topic | spinal cord injury silk fibroin silk biomaterials straining flow spinning axonal guidance regeneration |
| url | https://www.mdpi.com/2313-7673/8/1/65 |
| work_keys_str_mv | AT cristinacastrodominguez axonalguidanceusingbiofunctionalizedstrainingflowspinningregeneratedsilkfibroinfibersasscaffold AT palomalozanopicazo axonalguidanceusingbiofunctionalizedstrainingflowspinningregeneratedsilkfibroinfibersasscaffold AT aroaalvarezlopez axonalguidanceusingbiofunctionalizedstrainingflowspinningregeneratedsilkfibroinfibersasscaffold AT javiergarrotejunco axonalguidanceusingbiofunctionalizedstrainingflowspinningregeneratedsilkfibroinfibersasscaffold AT fivospanetsos axonalguidanceusingbiofunctionalizedstrainingflowspinningregeneratedsilkfibroinfibersasscaffold AT gustavovguinea axonalguidanceusingbiofunctionalizedstrainingflowspinningregeneratedsilkfibroinfibersasscaffold AT manuelelices axonalguidanceusingbiofunctionalizedstrainingflowspinningregeneratedsilkfibroinfibersasscaffold AT franciscojavierrojo axonalguidanceusingbiofunctionalizedstrainingflowspinningregeneratedsilkfibroinfibersasscaffold AT danielgonzaleznieto axonalguidanceusingbiofunctionalizedstrainingflowspinningregeneratedsilkfibroinfibersasscaffold AT luiscolchero axonalguidanceusingbiofunctionalizedstrainingflowspinningregeneratedsilkfibroinfibersasscaffold AT milagrosramos axonalguidanceusingbiofunctionalizedstrainingflowspinningregeneratedsilkfibroinfibersasscaffold AT joseperezrigueiro axonalguidanceusingbiofunctionalizedstrainingflowspinningregeneratedsilkfibroinfibersasscaffold |