Comprehensive Analysis of FASN in Tumor Immune Infiltration and Prognostic Value for Immunotherapy and Promoter DNA Methylation
Fatty acid synthase (FASN) promotes tumor progression in multiple cancers. In this study, we comprehensively examined the expression, prognostic significance, and promoter methylation of FASN, and its correlation with immune cell infiltration in pan-cancer. Our results demonstrated that elevated FAS...
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/24/15603 |
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| author | Mingyang Zhang Lei Yu Yannan Sun Li Hao Jing Bai Xinyu Yuan Rihan Wu Mei Hong Pengxia Liu Xiaojun Duan Changshan Wang |
| author_facet | Mingyang Zhang Lei Yu Yannan Sun Li Hao Jing Bai Xinyu Yuan Rihan Wu Mei Hong Pengxia Liu Xiaojun Duan Changshan Wang |
| author_sort | Mingyang Zhang |
| collection | DOAJ |
| description | Fatty acid synthase (FASN) promotes tumor progression in multiple cancers. In this study, we comprehensively examined the expression, prognostic significance, and promoter methylation of FASN, and its correlation with immune cell infiltration in pan-cancer. Our results demonstrated that elevated FASN expression was significantly associated with an unfavorable prognosis in many cancer types. Furthermore, FASN promoter DNA methylation can be used as a tumor prognosis marker. Importantly, high levels of FASN were significantly negatively correlated with tumor immune infiltration in 35 different cancers. Additionally, FASN was significantly associated with tumor mutational burden (TMB) and microsatellite instability (MSI) in multiple malignancies, suggesting that it may be essential for tumor immunity. We also investigated the effects of FASN expression on immunotherapy efficacy and prognosis. In up to 15 tumors, it was significantly negatively correlated with immunotherapy-related genes, such as PD-1, PD-L1, and CTLA-4. Moreover, we found that tumors with high FASN expression may be more sensitive to immunotherapy and have a good prognosis with PD-L1 treatment. Finally, we confirmed the tumor-suppressive effect of mir-195-5p through FASN. Altogether, our results suggested that FASN may serve as a novel prognostic indicator and immunotherapeutic target in various malignancies. |
| first_indexed | 2024-03-09T16:19:55Z |
| format | Article |
| id | doaj.art-9d809ad92341432a9a9c5be2f65226b7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:19:55Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9d809ad92341432a9a9c5be2f65226b72023-11-24T15:24:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123241560310.3390/ijms232415603Comprehensive Analysis of FASN in Tumor Immune Infiltration and Prognostic Value for Immunotherapy and Promoter DNA MethylationMingyang Zhang0Lei Yu1Yannan Sun2Li Hao3Jing Bai4Xinyu Yuan5Rihan Wu6Mei Hong7Pengxia Liu8Xiaojun Duan9Changshan Wang10School of Life Science, Inner Mongolia University, Hohhot 010020, ChinaSchool of Life Science, Inner Mongolia University, Hohhot 010020, ChinaSchool of Life Science, Inner Mongolia University, Hohhot 010020, ChinaSchool of Life Science, Inner Mongolia University, Hohhot 010020, ChinaSchool of Life Science, Inner Mongolia University, Hohhot 010020, ChinaSchool of Life Science, Inner Mongolia University, Hohhot 010020, ChinaSchool of Life Science, Inner Mongolia University, Hohhot 010020, ChinaSchool of Life Science, Inner Mongolia University, Hohhot 010020, ChinaSchool of Life Science, Inner Mongolia University, Hohhot 010020, ChinaSchool of Life Science, Inner Mongolia University, Hohhot 010020, ChinaSchool of Life Science, Inner Mongolia University, Hohhot 010020, ChinaFatty acid synthase (FASN) promotes tumor progression in multiple cancers. In this study, we comprehensively examined the expression, prognostic significance, and promoter methylation of FASN, and its correlation with immune cell infiltration in pan-cancer. Our results demonstrated that elevated FASN expression was significantly associated with an unfavorable prognosis in many cancer types. Furthermore, FASN promoter DNA methylation can be used as a tumor prognosis marker. Importantly, high levels of FASN were significantly negatively correlated with tumor immune infiltration in 35 different cancers. Additionally, FASN was significantly associated with tumor mutational burden (TMB) and microsatellite instability (MSI) in multiple malignancies, suggesting that it may be essential for tumor immunity. We also investigated the effects of FASN expression on immunotherapy efficacy and prognosis. In up to 15 tumors, it was significantly negatively correlated with immunotherapy-related genes, such as PD-1, PD-L1, and CTLA-4. Moreover, we found that tumors with high FASN expression may be more sensitive to immunotherapy and have a good prognosis with PD-L1 treatment. Finally, we confirmed the tumor-suppressive effect of mir-195-5p through FASN. Altogether, our results suggested that FASN may serve as a novel prognostic indicator and immunotherapeutic target in various malignancies.https://www.mdpi.com/1422-0067/23/24/15603FASNimmune infiltrationimmunotherapyDNA methylationmiRNApan-cancer |
| spellingShingle | Mingyang Zhang Lei Yu Yannan Sun Li Hao Jing Bai Xinyu Yuan Rihan Wu Mei Hong Pengxia Liu Xiaojun Duan Changshan Wang Comprehensive Analysis of FASN in Tumor Immune Infiltration and Prognostic Value for Immunotherapy and Promoter DNA Methylation International Journal of Molecular Sciences FASN immune infiltration immunotherapy DNA methylation miRNA pan-cancer |
| title | Comprehensive Analysis of FASN in Tumor Immune Infiltration and Prognostic Value for Immunotherapy and Promoter DNA Methylation |
| title_full | Comprehensive Analysis of FASN in Tumor Immune Infiltration and Prognostic Value for Immunotherapy and Promoter DNA Methylation |
| title_fullStr | Comprehensive Analysis of FASN in Tumor Immune Infiltration and Prognostic Value for Immunotherapy and Promoter DNA Methylation |
| title_full_unstemmed | Comprehensive Analysis of FASN in Tumor Immune Infiltration and Prognostic Value for Immunotherapy and Promoter DNA Methylation |
| title_short | Comprehensive Analysis of FASN in Tumor Immune Infiltration and Prognostic Value for Immunotherapy and Promoter DNA Methylation |
| title_sort | comprehensive analysis of fasn in tumor immune infiltration and prognostic value for immunotherapy and promoter dna methylation |
| topic | FASN immune infiltration immunotherapy DNA methylation miRNA pan-cancer |
| url | https://www.mdpi.com/1422-0067/23/24/15603 |
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