Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018
ABSTRACTHuman coronavirus NL63 (HCoV-NL63) is primarily associated with common cold in children, elderly and immunocompromised individuals. Outbreaks caused by HCoV-NL63 are rare. Here we report a cluster of HCoV-NL63 cases with severe lower respiratory tract infection that arose in Guangzhou, China...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1717999 |
| _version_ | 1797265883985346560 |
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| author | Yanqun Wang Xin Li Wenkuan Liu Mian Gan Lu Zhang Jin Wang Zhaoyong Zhang Airu Zhu Fang Li Jing Sun Guoxian Zhang Zhen Zhuang Jiaying Luo Dehui Chen Shuyan Qiu Li Zhang Duo Xu Chris Ka Pun Mok Fuchun Zhang Jingxian Zhao Rong Zhou Jincun Zhao |
| author_facet | Yanqun Wang Xin Li Wenkuan Liu Mian Gan Lu Zhang Jin Wang Zhaoyong Zhang Airu Zhu Fang Li Jing Sun Guoxian Zhang Zhen Zhuang Jiaying Luo Dehui Chen Shuyan Qiu Li Zhang Duo Xu Chris Ka Pun Mok Fuchun Zhang Jingxian Zhao Rong Zhou Jincun Zhao |
| author_sort | Yanqun Wang |
| collection | DOAJ |
| description | ABSTRACTHuman coronavirus NL63 (HCoV-NL63) is primarily associated with common cold in children, elderly and immunocompromised individuals. Outbreaks caused by HCoV-NL63 are rare. Here we report a cluster of HCoV-NL63 cases with severe lower respiratory tract infection that arose in Guangzhou, China, in 2018. Twenty-three hospitalized children were confirmed to be HCoV-NL63 positive, and most of whom were hospitalized with severe pneumonia or acute bronchitis. Whole genomes of HCoV-NL63 were obtained using next-generation sequencing. Phylogenetic and single amino acid polymorphism analyses showed that this outbreak was associated with two subgenotypes (C3 and B) of HCoV-NL63. Half of patients were identified to be related to a new subgenotype C3. One unique amino acid mutation at I507 L in spike protein receptor binding domain (RBD) was detected, which segregated this subgenotype C3 from other known subgenotypes. Pseudotyped virus bearing the I507 L mutation in RBD showed enhanced entry into host cells as compared to the prototype virus. This study proved that HCoV-NL63 was undergoing continuous mutation and has the potential to cause severe lower respiratory disease in humans. |
| first_indexed | 2024-03-07T17:24:13Z |
| format | Article |
| id | doaj.art-9d852b9b012d4a8a87cd04d0e572a8db |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-04-25T00:51:53Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-9d852b9b012d4a8a87cd04d0e572a8db2024-03-11T16:04:24ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-019124625510.1080/22221751.2020.1717999Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018Yanqun Wang0Xin Li1Wenkuan Liu2Mian Gan3Lu Zhang4Jin Wang5Zhaoyong Zhang6Airu Zhu7Fang Li8Jing Sun9Guoxian Zhang10Zhen Zhuang11Jiaying Luo12Dehui Chen13Shuyan Qiu14Li Zhang15Duo Xu16Chris Ka Pun Mok17Fuchun Zhang18Jingxian Zhao19Rong Zhou20Jincun Zhao21State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaInstitute of Infectious disease, Guangzhou Eighth People's Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaInstitute of Infectious disease, Guangzhou Eighth People's Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaABSTRACTHuman coronavirus NL63 (HCoV-NL63) is primarily associated with common cold in children, elderly and immunocompromised individuals. Outbreaks caused by HCoV-NL63 are rare. Here we report a cluster of HCoV-NL63 cases with severe lower respiratory tract infection that arose in Guangzhou, China, in 2018. Twenty-three hospitalized children were confirmed to be HCoV-NL63 positive, and most of whom were hospitalized with severe pneumonia or acute bronchitis. Whole genomes of HCoV-NL63 were obtained using next-generation sequencing. Phylogenetic and single amino acid polymorphism analyses showed that this outbreak was associated with two subgenotypes (C3 and B) of HCoV-NL63. Half of patients were identified to be related to a new subgenotype C3. One unique amino acid mutation at I507 L in spike protein receptor binding domain (RBD) was detected, which segregated this subgenotype C3 from other known subgenotypes. Pseudotyped virus bearing the I507 L mutation in RBD showed enhanced entry into host cells as compared to the prototype virus. This study proved that HCoV-NL63 was undergoing continuous mutation and has the potential to cause severe lower respiratory disease in humans.https://www.tandfonline.com/doi/10.1080/22221751.2020.1717999human coronavirus NL63new subgenotypepneumoniawhole-genome sequencingphylogenetic analysisviral entry |
| spellingShingle | Yanqun Wang Xin Li Wenkuan Liu Mian Gan Lu Zhang Jin Wang Zhaoyong Zhang Airu Zhu Fang Li Jing Sun Guoxian Zhang Zhen Zhuang Jiaying Luo Dehui Chen Shuyan Qiu Li Zhang Duo Xu Chris Ka Pun Mok Fuchun Zhang Jingxian Zhao Rong Zhou Jincun Zhao Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018 Emerging Microbes and Infections human coronavirus NL63 new subgenotype pneumonia whole-genome sequencing phylogenetic analysis viral entry |
| title | Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018 |
| title_full | Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018 |
| title_fullStr | Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018 |
| title_full_unstemmed | Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018 |
| title_short | Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018 |
| title_sort | discovery of a subgenotype of human coronavirus nl63 associated with severe lower respiratory tract infection in china 2018 |
| topic | human coronavirus NL63 new subgenotype pneumonia whole-genome sequencing phylogenetic analysis viral entry |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1717999 |
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