Opposing effects of trans‐ and cis‐cinnamic acid during rice coleoptile elongation
Abstract The phenylpropanoid cinnamic acid (CA) is a plant metabolite that can occur under a trans‐ or cis‐form. In contrast to the proven bioactivity of the cis‐form (c‐CA), the activity of trans‐CA (t‐CA) is still a matter of debate. We tested both compounds using a submerged rice coleoptile assay...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-12-01
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| Series: | Plant Direct |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pld3.465 |
| _version_ | 1828086218382901248 |
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| author | Lena Vlaminck Brix De Rouck Sandrien Desmet Thijs Van Gerrewey Geert Goeminne Lien De Smet Veronique Storme Tina Kyndt Kristof Demeestere Godelieve Gheysen Dirk Inzé Bartel Vanholme Stephen Depuydt |
| author_facet | Lena Vlaminck Brix De Rouck Sandrien Desmet Thijs Van Gerrewey Geert Goeminne Lien De Smet Veronique Storme Tina Kyndt Kristof Demeestere Godelieve Gheysen Dirk Inzé Bartel Vanholme Stephen Depuydt |
| author_sort | Lena Vlaminck |
| collection | DOAJ |
| description | Abstract The phenylpropanoid cinnamic acid (CA) is a plant metabolite that can occur under a trans‐ or cis‐form. In contrast to the proven bioactivity of the cis‐form (c‐CA), the activity of trans‐CA (t‐CA) is still a matter of debate. We tested both compounds using a submerged rice coleoptile assay and demonstrated that they have opposite effects on cell elongation. Notably, in the tip of rice coleoptile t‐CA showed an inhibiting and c‐CA a stimulating activity. By combining transcriptomics and (untargeted) metabolomics with activity assays and genetic and pharmacological experiments, we aimed to explain the underlying mechanistic processes. We propose a model in which c‐CA treatment activates proton pumps and stimulates acidification of the apoplast, which in turn leads to the loosening of the cell wall, necessary for elongation. We hypothesize that c‐CA also inactivates auxin efflux transporters, which might cause a local auxin accumulation in the tip of the coleoptile. For t‐CA, the phenotype can partially be explained by a stimulation of cell wall polysaccharide feruloylation, leading to a more rigid cell wall. Metabolite profiling also demonstrated that salicylic acid (SA) derivatives are increased upon t‐CA treatment. As SA is a known antagonist of auxin, the shift in SA homeostasis provides an additional explanation of the observed t‐CA‐mediated restriction on cell growth. |
| first_indexed | 2024-04-11T04:52:25Z |
| format | Article |
| id | doaj.art-9d87d8396a7447ea820c8014c8d6d92b |
| institution | Directory Open Access Journal |
| issn | 2475-4455 |
| language | English |
| last_indexed | 2024-04-11T04:52:25Z |
| publishDate | 2022-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj.art-9d87d8396a7447ea820c8014c8d6d92b2022-12-27T02:44:34ZengWileyPlant Direct2475-44552022-12-01612n/an/a10.1002/pld3.465Opposing effects of trans‐ and cis‐cinnamic acid during rice coleoptile elongationLena Vlaminck0Brix De Rouck1Sandrien Desmet2Thijs Van Gerrewey3Geert Goeminne4Lien De Smet5Veronique Storme6Tina Kyndt7Kristof Demeestere8Godelieve Gheysen9Dirk Inzé10Bartel Vanholme11Stephen Depuydt12Department of Plant Biotechnology and Bioinformatics Ghent University Ghent BelgiumLaboratory of Plant Growth Analysis Ghent University Global Campus Incheon South KoreaVIB Metabolomics Core Ghent Ghent BelgiumLaboratory of Plant Growth Analysis Ghent University Global Campus Incheon South KoreaVIB Metabolomics Core Ghent Ghent BelgiumDepartment of Biotechnology Ghent University Ghent BelgiumDepartment of Plant Biotechnology and Bioinformatics Ghent University Ghent BelgiumDepartment of Biotechnology Ghent University Ghent BelgiumDepartment of Green Chemistry and Technology Ghent University Ghent BelgiumDepartment of Biotechnology Ghent University Ghent BelgiumDepartment of Plant Biotechnology and Bioinformatics Ghent University Ghent BelgiumDepartment of Plant Biotechnology and Bioinformatics Ghent University Ghent BelgiumDepartment of Plant Biotechnology and Bioinformatics Ghent University Ghent BelgiumAbstract The phenylpropanoid cinnamic acid (CA) is a plant metabolite that can occur under a trans‐ or cis‐form. In contrast to the proven bioactivity of the cis‐form (c‐CA), the activity of trans‐CA (t‐CA) is still a matter of debate. We tested both compounds using a submerged rice coleoptile assay and demonstrated that they have opposite effects on cell elongation. Notably, in the tip of rice coleoptile t‐CA showed an inhibiting and c‐CA a stimulating activity. By combining transcriptomics and (untargeted) metabolomics with activity assays and genetic and pharmacological experiments, we aimed to explain the underlying mechanistic processes. We propose a model in which c‐CA treatment activates proton pumps and stimulates acidification of the apoplast, which in turn leads to the loosening of the cell wall, necessary for elongation. We hypothesize that c‐CA also inactivates auxin efflux transporters, which might cause a local auxin accumulation in the tip of the coleoptile. For t‐CA, the phenotype can partially be explained by a stimulation of cell wall polysaccharide feruloylation, leading to a more rigid cell wall. Metabolite profiling also demonstrated that salicylic acid (SA) derivatives are increased upon t‐CA treatment. As SA is a known antagonist of auxin, the shift in SA homeostasis provides an additional explanation of the observed t‐CA‐mediated restriction on cell growth.https://doi.org/10.1002/pld3.465bioactivitycinnamic acidcoleoptile elongationOryza sativa |
| spellingShingle | Lena Vlaminck Brix De Rouck Sandrien Desmet Thijs Van Gerrewey Geert Goeminne Lien De Smet Veronique Storme Tina Kyndt Kristof Demeestere Godelieve Gheysen Dirk Inzé Bartel Vanholme Stephen Depuydt Opposing effects of trans‐ and cis‐cinnamic acid during rice coleoptile elongation Plant Direct bioactivity cinnamic acid coleoptile elongation Oryza sativa |
| title | Opposing effects of trans‐ and cis‐cinnamic acid during rice coleoptile elongation |
| title_full | Opposing effects of trans‐ and cis‐cinnamic acid during rice coleoptile elongation |
| title_fullStr | Opposing effects of trans‐ and cis‐cinnamic acid during rice coleoptile elongation |
| title_full_unstemmed | Opposing effects of trans‐ and cis‐cinnamic acid during rice coleoptile elongation |
| title_short | Opposing effects of trans‐ and cis‐cinnamic acid during rice coleoptile elongation |
| title_sort | opposing effects of trans and cis cinnamic acid during rice coleoptile elongation |
| topic | bioactivity cinnamic acid coleoptile elongation Oryza sativa |
| url | https://doi.org/10.1002/pld3.465 |
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