Expression of cellular FLICE-like inhibitory protein in granulosa cells in In Vitro fertilization patients with advanced endometriosis
Background: This study evaluated the expression of cellular FLICE-like inhibitory protein (cFLIP) in granulosa cells (GCs) obtained from in vitro fertilization-embryo transfer patients with advanced endometriosis. Methods: A total of 267 patients with advanced endometriosis were enrolled in this stu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Health/LWW
2017-01-01
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| Series: | Reproductive and Developmental Medicine |
| Subjects: | |
| Online Access: | http://www.repdevmed.org/article.asp?issn=2096-2924;year=2017;volume=1;issue=2;spage=63;epage=68;aulast=Zhang |
| _version_ | 1811326785182236672 |
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| author | Jian-Ping Zhang Mei-Qin Yan Yao-Qin Wang Fang Yang Juan Liu Xue-Qing Wu |
| author_facet | Jian-Ping Zhang Mei-Qin Yan Yao-Qin Wang Fang Yang Juan Liu Xue-Qing Wu |
| author_sort | Jian-Ping Zhang |
| collection | DOAJ |
| description | Background: This study evaluated the expression of cellular FLICE-like inhibitory protein (cFLIP) in granulosa cells (GCs) obtained from in vitro fertilization-embryo transfer patients with advanced endometriosis.
Methods: A total of 267 patients with advanced endometriosis were enrolled in this study. They were divided into clinical pregnancy group (n = 114) and nonpregnancy group (n = 153). The expressions of cFLIP in mRNA and protein level were measured by real-time polymerase chain reaction (RT-PCR) and Western blotting. The related factors on the clinical pregnancy were analyzed using logistic regression analysis. Coefficients of correlation were calculated using the nonparametric rho-Spearman test.
Results: The number of oocytes retrieved, fertilization rate, and cleavage rate were significantly and independently related with clinical pregnancy (P < 0.05). RT-PCR and Western blotting analysis showed that the expressions of cFLP in mRNA and protein level were significantly higher in the clinical pregnancy group than in nonpregnancy group (P < 0.05). cFLIP had a significantly positive correlation with the number of oocytes retrieved (P < 0.05) and no correlation with fertilization rate and cleavage rate (P > 0.05).
Conclusion: Higher expression of cFLIP increased the pregnancy rate in women with advanced endometriosis. |
| first_indexed | 2024-04-13T14:55:32Z |
| format | Article |
| id | doaj.art-9d8cb036d03d4dcbad7f6b47353833ca |
| institution | Directory Open Access Journal |
| issn | 2096-2924 |
| language | English |
| last_indexed | 2024-04-13T14:55:32Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Health/LWW |
| record_format | Article |
| series | Reproductive and Developmental Medicine |
| spelling | doaj.art-9d8cb036d03d4dcbad7f6b47353833ca2022-12-22T02:42:27ZengWolters Kluwer Health/LWWReproductive and Developmental Medicine2096-29242017-01-0112636810.4103/2096-2924.216861Expression of cellular FLICE-like inhibitory protein in granulosa cells in In Vitro fertilization patients with advanced endometriosisJian-Ping ZhangMei-Qin YanYao-Qin WangFang YangJuan LiuXue-Qing WuBackground: This study evaluated the expression of cellular FLICE-like inhibitory protein (cFLIP) in granulosa cells (GCs) obtained from in vitro fertilization-embryo transfer patients with advanced endometriosis. Methods: A total of 267 patients with advanced endometriosis were enrolled in this study. They were divided into clinical pregnancy group (n = 114) and nonpregnancy group (n = 153). The expressions of cFLIP in mRNA and protein level were measured by real-time polymerase chain reaction (RT-PCR) and Western blotting. The related factors on the clinical pregnancy were analyzed using logistic regression analysis. Coefficients of correlation were calculated using the nonparametric rho-Spearman test. Results: The number of oocytes retrieved, fertilization rate, and cleavage rate were significantly and independently related with clinical pregnancy (P < 0.05). RT-PCR and Western blotting analysis showed that the expressions of cFLP in mRNA and protein level were significantly higher in the clinical pregnancy group than in nonpregnancy group (P < 0.05). cFLIP had a significantly positive correlation with the number of oocytes retrieved (P < 0.05) and no correlation with fertilization rate and cleavage rate (P > 0.05). Conclusion: Higher expression of cFLIP increased the pregnancy rate in women with advanced endometriosis.http://www.repdevmed.org/article.asp?issn=2096-2924;year=2017;volume=1;issue=2;spage=63;epage=68;aulast=ZhangAntral Follicle Count; Cellular FLICE-like Inhibitory Protein; Clinical Pregnancy; Endometriosis; In Vitro Fertilization-Embryo Transfer |
| spellingShingle | Jian-Ping Zhang Mei-Qin Yan Yao-Qin Wang Fang Yang Juan Liu Xue-Qing Wu Expression of cellular FLICE-like inhibitory protein in granulosa cells in In Vitro fertilization patients with advanced endometriosis Reproductive and Developmental Medicine Antral Follicle Count; Cellular FLICE-like Inhibitory Protein; Clinical Pregnancy; Endometriosis; In Vitro Fertilization-Embryo Transfer |
| title | Expression of cellular FLICE-like inhibitory protein in granulosa cells in In Vitro fertilization patients with advanced endometriosis |
| title_full | Expression of cellular FLICE-like inhibitory protein in granulosa cells in In Vitro fertilization patients with advanced endometriosis |
| title_fullStr | Expression of cellular FLICE-like inhibitory protein in granulosa cells in In Vitro fertilization patients with advanced endometriosis |
| title_full_unstemmed | Expression of cellular FLICE-like inhibitory protein in granulosa cells in In Vitro fertilization patients with advanced endometriosis |
| title_short | Expression of cellular FLICE-like inhibitory protein in granulosa cells in In Vitro fertilization patients with advanced endometriosis |
| title_sort | expression of cellular flice like inhibitory protein in granulosa cells in in vitro fertilization patients with advanced endometriosis |
| topic | Antral Follicle Count; Cellular FLICE-like Inhibitory Protein; Clinical Pregnancy; Endometriosis; In Vitro Fertilization-Embryo Transfer |
| url | http://www.repdevmed.org/article.asp?issn=2096-2924;year=2017;volume=1;issue=2;spage=63;epage=68;aulast=Zhang |
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