Oral SARS-CoV-2 Spike Protein Recombinant Yeast Candidate Prompts Specific Antibody and Gut Microbiota Reconstruction in Mice
A recent study showed that patients with coronavirus disease 2019 (COVID-19) have gastrointestinal symptoms and intestinal flora dysbiosis. Yeast probiotics shape the gut microbiome and improve immune homeostasis. In this study, an oral candidate of yeast-derived spike protein receptor-binding domai...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-04-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.792532/full |
| _version_ | 1811313183772639232 |
|---|---|
| author | Lilin Zhang Lan Yao Yanyu Guo Xiaoyang Li Li Ma Ruiqi Sun Xueqing Han Jing Liu Jinhai Huang |
| author_facet | Lilin Zhang Lan Yao Yanyu Guo Xiaoyang Li Li Ma Ruiqi Sun Xueqing Han Jing Liu Jinhai Huang |
| author_sort | Lilin Zhang |
| collection | DOAJ |
| description | A recent study showed that patients with coronavirus disease 2019 (COVID-19) have gastrointestinal symptoms and intestinal flora dysbiosis. Yeast probiotics shape the gut microbiome and improve immune homeostasis. In this study, an oral candidate of yeast-derived spike protein receptor-binding domain (RBD) and fusion peptide displayed on the surface of the yeast cell wall was generated. The toxicity and immune efficacy of oral administration were further performed in Institute of Cancer Research (ICR) mice. No significant difference in body weights, viscera index, and other side effects were detected in the oral-treated group. The detectable RBD-specific immunoglobulin G (IgG) and immunoglobulin A (IgA) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and more complex microbiota were detected from oral administration mice compared with those of the control group. Interestingly, the recombinant yeast was identified in female fetal of the high-dose group. These results revealed that the displaying yeast could fulfill the agent-driven immunoregulation and gut microbiome reconstitution. The findings will shed light on new dimensions against SARS-CoV-2 infection with the synergistic oral agents as promising non-invasive immunization and restoring gut flora. |
| first_indexed | 2024-04-13T10:49:23Z |
| format | Article |
| id | doaj.art-9d8e3bbe74874522a0409f77c4ba8405 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T10:49:23Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9d8e3bbe74874522a0409f77c4ba84052022-12-22T02:49:42ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-04-011310.3389/fmicb.2022.792532792532Oral SARS-CoV-2 Spike Protein Recombinant Yeast Candidate Prompts Specific Antibody and Gut Microbiota Reconstruction in MiceLilin Zhang0Lan Yao1Yanyu Guo2Xiaoyang Li3Li Ma4Ruiqi Sun5Xueqing Han6Jing Liu7Jinhai Huang8School of Life Sciences, Tianjin University, Tianjin, ChinaSchool of Life Sciences, Tianjin University, Tianjin, ChinaSchool of Life Sciences, Tianjin University, Tianjin, ChinaSchool of Life Sciences, Tianjin University, Tianjin, ChinaTianjin Institute of Pharmaceutical Research Co., Ltd., Tianjin, ChinaSchool of Life Sciences, Tianjin University, Tianjin, ChinaChinese Academy of Inspection and Quarantine, Beijing, ChinaTianjin Institute of Pharmaceutical Research Co., Ltd., Tianjin, ChinaSchool of Life Sciences, Tianjin University, Tianjin, ChinaA recent study showed that patients with coronavirus disease 2019 (COVID-19) have gastrointestinal symptoms and intestinal flora dysbiosis. Yeast probiotics shape the gut microbiome and improve immune homeostasis. In this study, an oral candidate of yeast-derived spike protein receptor-binding domain (RBD) and fusion peptide displayed on the surface of the yeast cell wall was generated. The toxicity and immune efficacy of oral administration were further performed in Institute of Cancer Research (ICR) mice. No significant difference in body weights, viscera index, and other side effects were detected in the oral-treated group. The detectable RBD-specific immunoglobulin G (IgG) and immunoglobulin A (IgA) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and more complex microbiota were detected from oral administration mice compared with those of the control group. Interestingly, the recombinant yeast was identified in female fetal of the high-dose group. These results revealed that the displaying yeast could fulfill the agent-driven immunoregulation and gut microbiome reconstitution. The findings will shed light on new dimensions against SARS-CoV-2 infection with the synergistic oral agents as promising non-invasive immunization and restoring gut flora.https://www.frontiersin.org/articles/10.3389/fmicb.2022.792532/fullSaccharomyces cerevisiaeSARS-CoV-2spike proteinoral administrationmicrobiome reconstruction |
| spellingShingle | Lilin Zhang Lan Yao Yanyu Guo Xiaoyang Li Li Ma Ruiqi Sun Xueqing Han Jing Liu Jinhai Huang Oral SARS-CoV-2 Spike Protein Recombinant Yeast Candidate Prompts Specific Antibody and Gut Microbiota Reconstruction in Mice Frontiers in Microbiology Saccharomyces cerevisiae SARS-CoV-2 spike protein oral administration microbiome reconstruction |
| title | Oral SARS-CoV-2 Spike Protein Recombinant Yeast Candidate Prompts Specific Antibody and Gut Microbiota Reconstruction in Mice |
| title_full | Oral SARS-CoV-2 Spike Protein Recombinant Yeast Candidate Prompts Specific Antibody and Gut Microbiota Reconstruction in Mice |
| title_fullStr | Oral SARS-CoV-2 Spike Protein Recombinant Yeast Candidate Prompts Specific Antibody and Gut Microbiota Reconstruction in Mice |
| title_full_unstemmed | Oral SARS-CoV-2 Spike Protein Recombinant Yeast Candidate Prompts Specific Antibody and Gut Microbiota Reconstruction in Mice |
| title_short | Oral SARS-CoV-2 Spike Protein Recombinant Yeast Candidate Prompts Specific Antibody and Gut Microbiota Reconstruction in Mice |
| title_sort | oral sars cov 2 spike protein recombinant yeast candidate prompts specific antibody and gut microbiota reconstruction in mice |
| topic | Saccharomyces cerevisiae SARS-CoV-2 spike protein oral administration microbiome reconstruction |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.792532/full |
| work_keys_str_mv | AT lilinzhang oralsarscov2spikeproteinrecombinantyeastcandidatepromptsspecificantibodyandgutmicrobiotareconstructioninmice AT lanyao oralsarscov2spikeproteinrecombinantyeastcandidatepromptsspecificantibodyandgutmicrobiotareconstructioninmice AT yanyuguo oralsarscov2spikeproteinrecombinantyeastcandidatepromptsspecificantibodyandgutmicrobiotareconstructioninmice AT xiaoyangli oralsarscov2spikeproteinrecombinantyeastcandidatepromptsspecificantibodyandgutmicrobiotareconstructioninmice AT lima oralsarscov2spikeproteinrecombinantyeastcandidatepromptsspecificantibodyandgutmicrobiotareconstructioninmice AT ruiqisun oralsarscov2spikeproteinrecombinantyeastcandidatepromptsspecificantibodyandgutmicrobiotareconstructioninmice AT xueqinghan oralsarscov2spikeproteinrecombinantyeastcandidatepromptsspecificantibodyandgutmicrobiotareconstructioninmice AT jingliu oralsarscov2spikeproteinrecombinantyeastcandidatepromptsspecificantibodyandgutmicrobiotareconstructioninmice AT jinhaihuang oralsarscov2spikeproteinrecombinantyeastcandidatepromptsspecificantibodyandgutmicrobiotareconstructioninmice |