Hepatitis E Virus Sequences in Swine Related to Sequences in Humans, the Netherlands
Hepatitis E virus (HEV), a major cause of viral hepatitis in much of the developing world, has recently been detected in swine in North America and Asia, raising concern about potential for zoonotic transmission. To investigate if HEV is commonly present in swine in the Netherlands, pooled stool sam...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2001-12-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/7/6/01-0608_article |
| _version_ | 1828373389211860992 |
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| author | Wim H.M. van der Poel Froukje Verschoor Reina van der Heide Maria-Inmaculada Herrera Amparo Vivo Marlou Kooreman Ana Maria de Roda Husman |
| author_facet | Wim H.M. van der Poel Froukje Verschoor Reina van der Heide Maria-Inmaculada Herrera Amparo Vivo Marlou Kooreman Ana Maria de Roda Husman |
| author_sort | Wim H.M. van der Poel |
| collection | DOAJ |
| description | Hepatitis E virus (HEV), a major cause of viral hepatitis in much of the developing world, has recently been detected in swine in North America and Asia, raising concern about potential for zoonotic transmission. To investigate if HEV is commonly present in swine in the Netherlands, pooled stool samples from 115 swine farms and nine individual pigs with diarrhea were assayed by reverse transcription-polymerase chain reaction (RT-PCR) amplification. HEV RNA was detected by RT-PCR and hybridization in 25 (22%) of the pooled specimens, but in none of the individual samples. RT-PCR amplification products of open reading frames 1 and 2 were sequenced, and the results were compared with published sequences of HEV genotypes from humans and swine. HEV strains from swine in the Netherlands were clustered in at least two groups, together with European and American isolates from swine and humans. Our data show that HEV in swine in the Netherlands are genetically closely related to HEV isolates from humans. Although zoonotic transmission has not been proven, these findings suggest that swine may be reservoir hosts of HEV. |
| first_indexed | 2024-04-14T07:18:08Z |
| format | Article |
| id | doaj.art-9d8f133e80754189903f10894424f96a |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-04-14T07:18:08Z |
| publishDate | 2001-12-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-9d8f133e80754189903f10894424f96a2022-12-22T02:06:15ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592001-12-017697097610.3201/eid0706.010608Hepatitis E Virus Sequences in Swine Related to Sequences in Humans, the NetherlandsWim H.M. van der PoelFroukje VerschoorReina van der HeideMaria-Inmaculada HerreraAmparo VivoMarlou KooremanAna Maria de Roda HusmanHepatitis E virus (HEV), a major cause of viral hepatitis in much of the developing world, has recently been detected in swine in North America and Asia, raising concern about potential for zoonotic transmission. To investigate if HEV is commonly present in swine in the Netherlands, pooled stool samples from 115 swine farms and nine individual pigs with diarrhea were assayed by reverse transcription-polymerase chain reaction (RT-PCR) amplification. HEV RNA was detected by RT-PCR and hybridization in 25 (22%) of the pooled specimens, but in none of the individual samples. RT-PCR amplification products of open reading frames 1 and 2 were sequenced, and the results were compared with published sequences of HEV genotypes from humans and swine. HEV strains from swine in the Netherlands were clustered in at least two groups, together with European and American isolates from swine and humans. Our data show that HEV in swine in the Netherlands are genetically closely related to HEV isolates from humans. Although zoonotic transmission has not been proven, these findings suggest that swine may be reservoir hosts of HEV.https://wwwnc.cdc.gov/eid/article/7/6/01-0608_articleHepatitus EHEVswinezoonosesthe Netherlands |
| spellingShingle | Wim H.M. van der Poel Froukje Verschoor Reina van der Heide Maria-Inmaculada Herrera Amparo Vivo Marlou Kooreman Ana Maria de Roda Husman Hepatitis E Virus Sequences in Swine Related to Sequences in Humans, the Netherlands Emerging Infectious Diseases Hepatitus E HEV swine zoonoses the Netherlands |
| title | Hepatitis E Virus Sequences in Swine Related to Sequences in Humans, the Netherlands |
| title_full | Hepatitis E Virus Sequences in Swine Related to Sequences in Humans, the Netherlands |
| title_fullStr | Hepatitis E Virus Sequences in Swine Related to Sequences in Humans, the Netherlands |
| title_full_unstemmed | Hepatitis E Virus Sequences in Swine Related to Sequences in Humans, the Netherlands |
| title_short | Hepatitis E Virus Sequences in Swine Related to Sequences in Humans, the Netherlands |
| title_sort | hepatitis e virus sequences in swine related to sequences in humans the netherlands |
| topic | Hepatitus E HEV swine zoonoses the Netherlands |
| url | https://wwwnc.cdc.gov/eid/article/7/6/01-0608_article |
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