Stacking of a low-lignin trait with an increased guaiacyl and 5-hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency in Arabidopsis thaliana
Abstract Background Lignocellulosic biomass, such as wood and straw, is an interesting feedstock for the production of fermentable sugars. However, mainly due to the presence of lignin, this type of biomass is recalcitrant to saccharification. In Arabidopsis, lignocellulosic biomass with a lower lig...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-018-1257-y |
| _version_ | 1818232796869558272 |
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| author | Lisanne de Vries Ruben Vanholme Rebecca Van Acker Barbara De Meester Lisa Sundin Wout Boerjan |
| author_facet | Lisanne de Vries Ruben Vanholme Rebecca Van Acker Barbara De Meester Lisa Sundin Wout Boerjan |
| author_sort | Lisanne de Vries |
| collection | DOAJ |
| description | Abstract Background Lignocellulosic biomass, such as wood and straw, is an interesting feedstock for the production of fermentable sugars. However, mainly due to the presence of lignin, this type of biomass is recalcitrant to saccharification. In Arabidopsis, lignocellulosic biomass with a lower lignin content or with lignin with an increased fraction of guaiacyl (G) and 5-hydroxyguaiacyl (5H) units shows an increased saccharification efficiency. Here, we stacked these two traits and studied the effect on the saccharification efficiency and biomass yield, by combining either transaldolase (tra2), cinnamate 4-hydroxylase (c4h-3), or 4-coumarate:CoA ligase (4cl1-1) with caffeic acid O-methyltransferase (comt-1 or comt-4) mutants. Results The three double mutants (tra2 comt-1, c4h-3 comt-4, and 4cl1-1 comt-4) had a decreased lignin amount and an increase in G and 5H units in the lignin polymer compared to wild-type (WT) plants. The tra2 comt-1 double mutant had a better saccharification efficiency compared to the parental lines when an acid or alkaline pretreatment was used. For the double mutants, c4h-3 comt-4 and 4cl1-1 comt-4, the saccharification efficiency was significantly higher compared to WT and its parental lines, independent of the pretreatment used. When no pretreatment was used, the saccharification efficiency increased even synergistically for these mutants. Conclusion Our results show that saccharification efficiency can be improved by combining two different mutant lignin traits, leading to plants with an even higher saccharification efficiency, without having a yield reduction of the primary inflorescence stem. This approach can help improve saccharification efficiency in bio-energy crops. |
| first_indexed | 2024-12-12T11:11:59Z |
| format | Article |
| id | doaj.art-9d8f5dc70a3642d1bae9f356e5439df8 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T11:11:59Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-9d8f5dc70a3642d1bae9f356e5439df82022-12-22T00:26:16ZengBMCBiotechnology for Biofuels1754-68342018-09-0111111410.1186/s13068-018-1257-yStacking of a low-lignin trait with an increased guaiacyl and 5-hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency in Arabidopsis thalianaLisanne de Vries0Ruben Vanholme1Rebecca Van Acker2Barbara De Meester3Lisa Sundin4Wout Boerjan5Department of Plant Biotechnology and Bioinformatics, Ghent UniversityDepartment of Plant Biotechnology and Bioinformatics, Ghent UniversityDepartment of Plant Biotechnology and Bioinformatics, Ghent UniversityDepartment of Plant Biotechnology and Bioinformatics, Ghent UniversityDepartment of Plant Biotechnology and Bioinformatics, Ghent UniversityDepartment of Plant Biotechnology and Bioinformatics, Ghent UniversityAbstract Background Lignocellulosic biomass, such as wood and straw, is an interesting feedstock for the production of fermentable sugars. However, mainly due to the presence of lignin, this type of biomass is recalcitrant to saccharification. In Arabidopsis, lignocellulosic biomass with a lower lignin content or with lignin with an increased fraction of guaiacyl (G) and 5-hydroxyguaiacyl (5H) units shows an increased saccharification efficiency. Here, we stacked these two traits and studied the effect on the saccharification efficiency and biomass yield, by combining either transaldolase (tra2), cinnamate 4-hydroxylase (c4h-3), or 4-coumarate:CoA ligase (4cl1-1) with caffeic acid O-methyltransferase (comt-1 or comt-4) mutants. Results The three double mutants (tra2 comt-1, c4h-3 comt-4, and 4cl1-1 comt-4) had a decreased lignin amount and an increase in G and 5H units in the lignin polymer compared to wild-type (WT) plants. The tra2 comt-1 double mutant had a better saccharification efficiency compared to the parental lines when an acid or alkaline pretreatment was used. For the double mutants, c4h-3 comt-4 and 4cl1-1 comt-4, the saccharification efficiency was significantly higher compared to WT and its parental lines, independent of the pretreatment used. When no pretreatment was used, the saccharification efficiency increased even synergistically for these mutants. Conclusion Our results show that saccharification efficiency can be improved by combining two different mutant lignin traits, leading to plants with an even higher saccharification efficiency, without having a yield reduction of the primary inflorescence stem. This approach can help improve saccharification efficiency in bio-energy crops.http://link.springer.com/article/10.1186/s13068-018-1257-ySaccharificationLigninGene stackingTransaldolase (tra)Cinnamate 4-hydroxylase (c4h)4-coumarate:CoA ligase (4cl) |
| spellingShingle | Lisanne de Vries Ruben Vanholme Rebecca Van Acker Barbara De Meester Lisa Sundin Wout Boerjan Stacking of a low-lignin trait with an increased guaiacyl and 5-hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency in Arabidopsis thaliana Biotechnology for Biofuels Saccharification Lignin Gene stacking Transaldolase (tra) Cinnamate 4-hydroxylase (c4h) 4-coumarate:CoA ligase (4cl) |
| title | Stacking of a low-lignin trait with an increased guaiacyl and 5-hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency in Arabidopsis thaliana |
| title_full | Stacking of a low-lignin trait with an increased guaiacyl and 5-hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency in Arabidopsis thaliana |
| title_fullStr | Stacking of a low-lignin trait with an increased guaiacyl and 5-hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency in Arabidopsis thaliana |
| title_full_unstemmed | Stacking of a low-lignin trait with an increased guaiacyl and 5-hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency in Arabidopsis thaliana |
| title_short | Stacking of a low-lignin trait with an increased guaiacyl and 5-hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency in Arabidopsis thaliana |
| title_sort | stacking of a low lignin trait with an increased guaiacyl and 5 hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency in arabidopsis thaliana |
| topic | Saccharification Lignin Gene stacking Transaldolase (tra) Cinnamate 4-hydroxylase (c4h) 4-coumarate:CoA ligase (4cl) |
| url | http://link.springer.com/article/10.1186/s13068-018-1257-y |
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