Effects of Kelulut Honey on Oestrus Cycle Regulation and Histomorphological Changes in Letrozole-Induced Polycystic Ovary Syndrome Rats: A Preliminary Study
Polycystic ovary syndrome (PCOS) is a complex reproductive, metabolic, and endocrine disorder that affects women of reproductive age. Kelulut honey is stingless bee honey that possesses anti-inflammatory, anti-cancer, anti-diabetic, and potent antioxidative activities in most conditions. However, it...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/12/6/890 |
| _version_ | 1827659172755275776 |
|---|---|
| author | Datu Agasi Mohd Kamal Siti Fatimah Ibrahim Azizah Ugusman Mohd Helmy Mokhtar |
| author_facet | Datu Agasi Mohd Kamal Siti Fatimah Ibrahim Azizah Ugusman Mohd Helmy Mokhtar |
| author_sort | Datu Agasi Mohd Kamal |
| collection | DOAJ |
| description | Polycystic ovary syndrome (PCOS) is a complex reproductive, metabolic, and endocrine disorder that affects women of reproductive age. Kelulut honey is stingless bee honey that possesses anti-inflammatory, anti-cancer, anti-diabetic, and potent antioxidative activities in most conditions. However, its value in improving PCOS remains to be elucidated. Thus, this preliminary study aimed to determine the effective dose of Kelulut honey in oestrus cycle regulation and ovarian histomorphological changes in letrozole-induced PCOS rats. PCOS was induced in all-female Sprague Dawley (SD) rats with 1 mg/kg/day of letrozole except for the control group for 21 days. Kelulut honey was then orally administered to the PCOS rats at the dose of 0.5, 1, or 2 g/kg/day, respectively, for 35 days. The oestrous cycle was determined through vaginal smears, while ovarian histomorphological changes were observed by haematoxylin and eosin (H&E) staining. The untreated PCOS rats were characterised by irregular oestrous cyclicity, hyperglycaemia, and aberrant ovarian histology. In this study, Kelulut honey (1 g/kg/day) increased the number of corpus luteum and antral follicles (<i>p</i> < 0.05), improved the cystic follicle, and normalised the oestrus cycle (<i>p</i> < 0.05). This preliminary study demonstrated that Kelulut honey, particularly at a dose of 1 g/kg/day, has the potential to alleviate oestrus cycle dysregulation and ovarian histomorphological changes occurring in PCOS. |
| first_indexed | 2024-03-09T23:15:47Z |
| format | Article |
| id | doaj.art-9d91080cf915472796bbaa28a167c8dc |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T23:15:47Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-9d91080cf915472796bbaa28a167c8dc2023-11-23T17:37:15ZengMDPI AGLife2075-17292022-06-0112689010.3390/life12060890Effects of Kelulut Honey on Oestrus Cycle Regulation and Histomorphological Changes in Letrozole-Induced Polycystic Ovary Syndrome Rats: A Preliminary StudyDatu Agasi Mohd Kamal0Siti Fatimah Ibrahim1Azizah Ugusman2Mohd Helmy Mokhtar3Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, MalaysiaDepartment of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, MalaysiaDepartment of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, MalaysiaDepartment of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, MalaysiaPolycystic ovary syndrome (PCOS) is a complex reproductive, metabolic, and endocrine disorder that affects women of reproductive age. Kelulut honey is stingless bee honey that possesses anti-inflammatory, anti-cancer, anti-diabetic, and potent antioxidative activities in most conditions. However, its value in improving PCOS remains to be elucidated. Thus, this preliminary study aimed to determine the effective dose of Kelulut honey in oestrus cycle regulation and ovarian histomorphological changes in letrozole-induced PCOS rats. PCOS was induced in all-female Sprague Dawley (SD) rats with 1 mg/kg/day of letrozole except for the control group for 21 days. Kelulut honey was then orally administered to the PCOS rats at the dose of 0.5, 1, or 2 g/kg/day, respectively, for 35 days. The oestrous cycle was determined through vaginal smears, while ovarian histomorphological changes were observed by haematoxylin and eosin (H&E) staining. The untreated PCOS rats were characterised by irregular oestrous cyclicity, hyperglycaemia, and aberrant ovarian histology. In this study, Kelulut honey (1 g/kg/day) increased the number of corpus luteum and antral follicles (<i>p</i> < 0.05), improved the cystic follicle, and normalised the oestrus cycle (<i>p</i> < 0.05). This preliminary study demonstrated that Kelulut honey, particularly at a dose of 1 g/kg/day, has the potential to alleviate oestrus cycle dysregulation and ovarian histomorphological changes occurring in PCOS.https://www.mdpi.com/2075-1729/12/6/890PCOSKelulut honeyovaryoestrous cycle |
| spellingShingle | Datu Agasi Mohd Kamal Siti Fatimah Ibrahim Azizah Ugusman Mohd Helmy Mokhtar Effects of Kelulut Honey on Oestrus Cycle Regulation and Histomorphological Changes in Letrozole-Induced Polycystic Ovary Syndrome Rats: A Preliminary Study Life PCOS Kelulut honey ovary oestrous cycle |
| title | Effects of Kelulut Honey on Oestrus Cycle Regulation and Histomorphological Changes in Letrozole-Induced Polycystic Ovary Syndrome Rats: A Preliminary Study |
| title_full | Effects of Kelulut Honey on Oestrus Cycle Regulation and Histomorphological Changes in Letrozole-Induced Polycystic Ovary Syndrome Rats: A Preliminary Study |
| title_fullStr | Effects of Kelulut Honey on Oestrus Cycle Regulation and Histomorphological Changes in Letrozole-Induced Polycystic Ovary Syndrome Rats: A Preliminary Study |
| title_full_unstemmed | Effects of Kelulut Honey on Oestrus Cycle Regulation and Histomorphological Changes in Letrozole-Induced Polycystic Ovary Syndrome Rats: A Preliminary Study |
| title_short | Effects of Kelulut Honey on Oestrus Cycle Regulation and Histomorphological Changes in Letrozole-Induced Polycystic Ovary Syndrome Rats: A Preliminary Study |
| title_sort | effects of kelulut honey on oestrus cycle regulation and histomorphological changes in letrozole induced polycystic ovary syndrome rats a preliminary study |
| topic | PCOS Kelulut honey ovary oestrous cycle |
| url | https://www.mdpi.com/2075-1729/12/6/890 |
| work_keys_str_mv | AT datuagasimohdkamal effectsofkeluluthoneyonoestruscycleregulationandhistomorphologicalchangesinletrozoleinducedpolycysticovarysyndromeratsapreliminarystudy AT sitifatimahibrahim effectsofkeluluthoneyonoestruscycleregulationandhistomorphologicalchangesinletrozoleinducedpolycysticovarysyndromeratsapreliminarystudy AT azizahugusman effectsofkeluluthoneyonoestruscycleregulationandhistomorphologicalchangesinletrozoleinducedpolycysticovarysyndromeratsapreliminarystudy AT mohdhelmymokhtar effectsofkeluluthoneyonoestruscycleregulationandhistomorphologicalchangesinletrozoleinducedpolycysticovarysyndromeratsapreliminarystudy |